The study on vertical transmision of Nam Dinh Virus and Coltivirus group B from Culex quinque fasciatus in Can Tho Province

Background:\r\n', u'There are two virus known as Nam Dinh Virus, and Colti group B be found in Viet Nam. These viruses have appeared in the South, the Middle and the Highland. They haven\u2019t been reported in the Southern provinces and Can Thoas well. \r\n', u'Objectives: \r\n', u'To identify the circulation of Nam Dinh virus strain, and coltivirus group B strain in Can Tho, Southern Viet Nam, and their existence in nature.\r\n', u'Subjects and method: \r\n', u'Thirty-four mosquito samples (7, 453 individual mosquitoes) from Culex quinque faciatus and Culex pseudovishnui were collected in Can Tho provice, southern Vietnam 2005.\r\n', u'Isolatingviruses on Aedes albopictuc clone C6/36, Vero cells, and using PT- PCR and ELISA Sandwich for identification. \r\n', u'Results:\r\n', u'2 Nam Dinh virus strains, 2 coltivirus group B strains and 1 flavivirus strain (insect flavivirus) were isolated from Culex quinque faciatus, and no virus was isolated from Culex pseudovishnui.\r\n', u'Conclusion: \r\n', u'The identification of the transmission of Nam dinh Virus, and coltivirus group B in Can Tho province by isolating virus from Culex quinque faciatus has shown the evidence for natural vertical transmission of these viruses.\r\n', u'

Applying mac-elisa for etiological surveillance of Japanese encephalitis, West Nile and Nam Dinh viruses cause acute encephalitis syndrome, 2003-2004

During 2003 - 2004, in total 976 sera and cerebrospinal fluid samples from patients with acute encephalitis syndrome (AES) were collected from Pediatric Hospital, several Centers of Preventive Medicine and National Institute of Hygiene and Epidemiology, Tay Nguyen for screening viral pathogens cause AES using MAC-ELISA with Japanese encephalitis (JE), West Nile and Nam Dinh virus antigens. Results: Out of 976 sera and cerebrospinal fluid samples of AES patients were screened using JE virus antigen, 46.1% (454/976) cases were confirmed to be JE virus infection. Out of 239 cerebrospinal fluid samples of AES patients were screened by Nam Dinh virus antigen, 13.4% (32/239) cases were confirmed to be Nam Dinh virus infection. Out of 103 sera and cerebrospinal fluid samples of AES patients were screened by West Nile virus antigen, there were no positive result was confirmed with this antigen. To date, JE virus is leading cause of AES in Vietnam, with over 80% cases in children under 15 years old. It should be intensive JE vaccination for all children under 15 years old in order to control JE soon.