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Silica based nanoparticles are used in various fields of medical sciences to diagnose, control disease, for genetic disorders, owing to their size, surface area, biocompatibility and low toxicity. In dentistry, silica nanoparticles have been used as dental filler, teeth whitening agent but limited evidence is there regarding antimicrobial activity against oral pathogens. Therefore, the current study was conducted to assess the anti-bacterial activity of mouthwash incorporated with silica nanoparticles against oral pathogens. Tetraethoxysilane, ammonium hydroxide, absolute ethanol were used and centrifuged to obtain the silica nanoparticle pellet. XRD analysis was done to confirm the characterization of the thus obtained silica nanoparticle. The mouthwash was prepared with the synthesized silica nanoparticle as the main constituent. Agar well diffusion method was used to assess the antimicrobial activity against S. mutans, S. aureus and E. faecalis. The XRD analysis confirmed the amorphous nature of the synthesized silica nanoparticles.The zone of inhibition was found to increase as the concentrations increased mainly for S. aureus and E. faecalis. The synthesized nanoparticles incorporated mouthwash showed good potential as antimicrobial agents against strains of gram positive bacteria. Further animal studies/in vivo research should be conducted to validate the above findings
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Background: Nanotechnology can be defined as the science involved in the design, synthesis, characterization, and application of materials and devices whose smallest functional organization in at least one dimension is on the nanometer scale. Aim: The aim of this cross-sectional study was to assess the knowledge and attitude of dental students and intern practitioners about the nanotechnology in dentistry at KSA. Materials and methods: An online questionnaire was distributed through the link shared to dental students and intern practitioners via social networking websites. A simple random sample comprised of 306 participants almost distributed across the five geographical regions of Saudi Arabia. The questionnaire was designed to obtain information about the basic knowledge and attitude about nanotechnology being used worldwide for better dental treatment. It consisted of two parts: Part A: Biographic and demographic data of the participants. Part B: Knowledge and Attitude towards nanotechnology in dentistry was focused. Statistical analysis of the data was performed. Frequency distribution and Chi-square analysis were used to determine if there were any relationships and statistically significant differences between knowledge and attitude of nanotechnology in dentistry with biographic and demographic factors. Ola M. Sakr and Alhablain, E.A. Assessment of the Knowledge and Attitude of Dental Students and Intern Practitioners about the Nanotechnology in Dentistry at KSA. IAIM, 2018; 5(10): 87-94. Page 88 Results: Out of the 306 participants' response, 213 (69.3%) had heard about the nanotechnology and the main source of information about nanotechnology in dentistry was Internet (32.1%). Only (26.4%) of participates use nanomaterial in their university and (73.6 %) didn't use it. (52.1%) of them strongly disagree with nanotechnology will have a wide application in future. Conclusion: This study revealed that there is lack of awareness on Nanotechnology application in dentistry among the dental students and intern practitioners at Saudi Arabia.
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Objetivo: El objetivo de esta investigación fue valorar la eficacia del activo Nbelyax del esterilizante en frío Éviter™ con presencia de nanotecnología en tres diferentes cepas bacterianas que son de importancia en el sector odontológico: E. faecalis, S. aureus y Neisseria sp. Materiales y métodos: se realizó un estudio experimental, observacional, cualitativo empleando las tres cepas. Se preparó el producto esterilizante Éviter a una dilución de 1:20. En un matraz Erlenmeyer de 250 mL se colocaron 99 mL del producto preparado, donde se inoculó cada cepa bacteriana, se dejó en contacto a dos tiempos diferentes: 5 y 30 minutos. Posteriormente se hizo el vaciado en placa de alícuota de 0,1 mL en 18 mL de soya tripticasa por cada caja de Petri, se incubó 48 horas a 37 °C, y se montó el experimento por duplicado. Para el análisis de resultados se utilizó el programa estadístico SPSS versión 20.0 aplicando la prueba estadística McNemar. Resultados: mostraron que después de 5 minutos de contacto con el esterilizante en frío Éviter, las cepas E. faecalis y S. aureus presentaron desarrollo bacteriano; sin embargo, Neisseria sp. no mostró desarrollo. Las tres cepas bacterianas después de 30 minutos de contacto no presentaron desarrollo colonial. Al aplicarse la prueba estadística McNemar no hubo diferencias estadísticamente significativas (p?#8805;0,05). Con la muestra y metodología usada, se observó que el esterilizante quirúrgico en frío Éviter fue eficaz para eliminar los microorganismos de prueba en 30 minutos.
Purpose: The goal of this research was to evaluate the effectiveness of the active Nbelyax cold sterilant (Éviter™) with nanotechnology in three different bacterial strains of great importance in dentistry: E. faecalis, S. aureus and Neisseria sp. Materials and Method: An experimental, observational and qualitative study using three different bacterial strains was performed. The sterilant product was prepared at 1: 20 dilution. Then, in a 250 mL Erlenmeyer flask were poured 99 mL of the sterilant prepared, each bacterial strain was inoculated in same flask, allowing two different contact times: 5 and 30 minutes. Subsequently, an aliquot of 0.1 mL in 18 mL of trypticase soy per petri plaque was done using pour plate technique and incubated 48 hours at 37 °C. The experiment was done by duplicate. The SPSS statistical program version 20.0 was used, applying Mcnemar statistical test to analyze the results. Results: This research showed that after 5 minutes of contact with the cold sterilant Éviter, E. faecalis and S. aureus strains had bacterial growth, however Neisseria sp. did not have bacterial colonies growth. The three bacterial strains after 30 minutes of contact did not show colonies growth. McNemar test did not show significant statistical differences (p?#8805;0.05). Conclusions: With the sample and ramimethodology used, we observed that the recommended contact time of the cold sterilant Éviter product with nanotechnology to eliminate the microorganism tasted was 30 minutes.
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As with all emerging technologies, a successful future for nanotechnology will only be achieved through open sharing of ideas and research findings, through testing and frank discussion. Time, advances, resources, and needs will determine which prospects becomes reality. Application of nanotechnology to all branches of dentistry will soon be a reality. So get ready for smart nanotech dentistry.
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The future trend in dentistry is nanotechnology, aptly named as, Nanodentistry. Nanotechnology is a technology which manipulates matter at a nanometer level, and is increasingly finding its application in treatment of cancer, ranging from early detection, accurate staging at a microtumour level and therapy. This technology offers a shift from non specific, highly toxic and expensive drugs to highly specific, less toxic and inexpensive drugs. Nanomedicine breaches the walls of conventional medicine, by being more soluble, site specific and with a longer duration of action. Thus, this review aims to summarize in a nutshell the future of nanotechnology in dentistry with key emphasis on its role as an anticancer modality
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Actualmente la nano-odontología pareciera una situación de fi cción, sin embargo los avancescientífi cos en la nanotecnología para la mejor comprensión de la relación que existe entre las nanoestructuras de las piezas dentarias y los microorganismos colonizadores, abre una inmensavariedad de oportunidades y posibilidades que podrían revolucionar el mundo de la odontología,como es el que se podría mantener una salud oral integral, mediante el empleo de biomateriales,incorporando a la ingeniería tisular y nanorobots. Cuando los nanorobots dentales puedan ser manufacturados en 5 a 10 años, estos instrumentos permitirán el control exacto de la anestesia oral, el reemplazo dental y la odontología reconstructiva de precisión a escala nanométrica. Asimismonuevos prospectos terapéuticos serían factibles utilizando los dentrifi corobots monitorizados. En este artículo se hace una prospectiva y revisión de las aplicaciones de la nanotecnología en laodontología, y cómo la nanotecnología esta permitiendo un gran aporte al desarrollo de nuevos mecanismos y materiales en la medicina oral.
Currently, nano-dentistry would seem to be a fi ctional topic, but scientifi c advances in nanotechnology in order to better understand the relationship between tooth nanostructures and colonizing microorganisms provides a vast array of opportunities and possibilities that could revolutionize the world of dentistry. Complete oral health through the use of biomaterials, tissue engineering and nanorobots could be possible. When dental nanorobots can be manufactured in 5 to 10 years, these instruments will allow specifi cally controlled oral analgesia, dental replacement and nanometerscaleprecision restorative dentistry. New therapeutic prospects will be feasible utilizing motorized dentirobots. This article is a prospective review of the applications of molecular engineering techniques in dentistry and how these new tools can provide valuable support to the developmentof new dental biomaterials.
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Humanos , Nanotecnología , Medicina OralRESUMEN
El desarrollo científico sin precedentes alcanzado en los últimos años por la nanotecnología ha generado una nueva dimensión en el desarrollo tecnológico, médico y económico que ha marcado un impacto global. Los avances en estrategias de diagnóstico, tratamiento, y prevención de las enfermedades preveen un importante rol de la nanotecnología en el emergente campo aplicado a la odontología denominado nanoodontología. Entre sus principales desafíos destaca el alcanzar una mejor comprensión de las bases fisiopatológicas de las enfermedades, nuevas estrategias de diagnóstico, y el desarrollo de terapias altamente efectivas. La nanotecnología indica ser el camino de la evolución, y el desarrollo de la odontología del siglo 21. Este artículo, presenta las tendencias, y desafíos de nanotecnología aplicada a la odontología, provee de una visión global del desarrollo de la nanoodontología y su impacto futuro.
The unprecedented scientific development achieved in recent years by the nanotechnology has created a new dimension in the technological, medical and economic development set a global impact. Advances in strategies for diagnosis, treatment, and prevention of diseases foresee an important role of the nanotechnology in the emerging field applied to dentistry called nanodentistry. Among its highlights the main challenges to achieve a better understanding of the pathophysiological bases of diseases, new diagnostic strategies, and development of highly effective therapies. Nanotechnology shows to be the path of evolution, and development of dentistry in the 21st century. This article presents the trends and challenges of nanotechnology applied to dentistry, provides an overview of nanodentistry development and its future impact.