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Objective To study the effects of rAAV2/1-Acrp30 on sICAM-1 and sVCAM-1 level as well as NF-?B expression in GK rats with diabetic arteriosclerosis so as to explore the effect of adiponectin on diabetic macroangiopathy. Methods A total number of 30 atherosclerotic GK rat models were randomly divided into three groups:① Model group one (M1):hind limb intramuscular injection of normal saline; ② Model group two (M2):hind limb intramuscular injection of vacuity virus rAAV2/1; ③ Treatment group (T):hind limb intramuscular injection of rAAV2/1-Acrp30 at a dose of 1?1012mg/L. After 8 weeks' treatment,the rats were killed,and serum sVCAM-1 and sICAM-1 level as well as aortic NF-?Bp65mRNA expression were measured in each group. Aortic NF-?Bp65mRNA expression was measured by RT-PCR.Results Compared with those in control model groups (M1 and M2),sVCAM-1 and sICAM-1 levels were decreased significantly in the treatment (T) group (P
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Objective To explore the expression of NF-?B in C-7-T-5 spinal ganglia and possible mechanism of treating asthma with dexamethsone. Methods The alterations of NF-?B activity and its mRNA expression were investigated by means of immunohistochemistry and in situ hybridization histochemistry combined with the micro-image analysis in C-7-T-5 spinal ganglia in the asthmatic and dexamethsone-treated guinea pigs. Results The NF-?B immunoreactivity was found mainly in cytoplasm and weakly in nucleus,its mRNA was expressed weakly in C-7-T-5 spinal ganglia in the control groups.The NF-?B immunoreactivity was more in nucleus and less in cytoplasm,the expression of its mRNA increased significantly in C-7-T-5 spinal ganglia in the asthmatic group compared with the control groups(P0.05).Conclusion The present results indicate that NF-?B in C-7-T-5 spinal ganglia might be involved in the pathogenesis of the bronchial asthma,and inhibiting the expression and activity of NF-?B in C-7-T-5 spinal ganglia might be one of the mechanisms of treating the bronchial asthma with dexamethsone.