RESUMEN
OBJECTIVE: To establish an HPLC method for simultaneous determination of 12 constituents in Nüjin pills. METHODS: The analysis was carried out on Agilent Eclipse plus C18 column(4.6 mm×250 mm,5 μm), using a mobile phase of acetonitrile (A)-0.2%formic acid solution (B) by a gradient elution program (0-25 min, 10%-35% A; 25-37 min, 35%-59% A; 37-40 min, 59%-75% A) at a flow rate of 1 mL•min-1 at 30 ℃. The detection wavelength was set at 230 nm in the first 20 min, and then changed to 270 nm between 20 and 40 min. RESULTS: The linear ranges of paeoniflorin, liquiritin, ferulicacid, narirutin, aurantiamarin, baicalin, wogonoside, cinnam aldehyde, baicalein, paeonol, ammonium glycyrrhetate and wogonin fell within the ranges of 0.011-1.062 μg, 0.002-0.237 μg, 0.003-0.259 μg, 0.006-0.641 μg, 0.029-2.925 μg, 0.033-3.339 μg, 0.008-0.834 μg, 0.003-0.258 μg, 0.008-0.772 μg, 0.008-0.824 μg, 0.007-0.724 μg and 0.005-0.539 μg, respectively. The recoveries were 99.3%, 98.4%, 99.1%, 99.4%, 99.5%, 99.6%, 99.4%, 99.2%, 99.0%, 99.6%, 99.1%, and 99.4%, respectively. The relative standard deviations were 0.35%, 1.02%, 0.33%, 0.38%, 0.18%, 0.27%, 0.40%, 0.74%, 0.53%, 0.16%, 0.08% and 0.21%, respectively (n=6). CONCLUSION: This method is simple, accurate and convenient for the quality control of Nüjin pills.