RESUMEN
Background: The global prevalence of developmental delay in children is reported as 1-3% according to WHO. Early diagnosis and intervention improve the quality of life in disabled children. There is need for screening tool. So, study was started to evaluate the ability of the online screening tool in detecting the developmental delay in the Indian children.Methods: After IRB approval, around 30 children after parental consent of either gender aged 4 months to 5 years attending the immunization outpatient department were involved in the study After a detailed history, children were screened with online screening tool 'Track and Act'. Due to the logistic reasons among them 30 were assessed in detail using Developmental Assessment Scale for Indian Infants (DASII) which is used as gold standard in this study.Results: Study showed high sensitivity and specificity for all the four domains of the tool. It showed sensitivity and specificity in physical of 83.3% and 91.6%, in language 66.6% and 87.5%, in cognitive 66.6% and 91.6% and in socio-emotional domain 83.3% and 91.6 % respectively.Conclusions: Track and Act screening tool has good test characteristics for detecting developmental delay among Indian children and it can be used for office practice for screening children.
RESUMEN
Objective: To establish a method for rapid screening and identifying antioxidants in natural products by ultra high performance liquid chromatography-(1,1-dipheny l-2-trinitrophenylhydrazine)-photo diode array-electrospray-time-of-flight mass spectrometry (UPLC-DPPH-PDA-ESI-TOF/MS) and apply it to the rapid discovery of antioxidants from Salviamiltiorrhiza extracts. Methods: The Waters ACQUITY UPLC system and a Waters ACQUITY UPLC HSS T3 column (100 mm×3.0mm,1.8 μm; Waters Technologies, USA) were used to elute the sample at a low flow rate of 0.35 mL/min, which could achieve the rapid separation of S. miltiorrhiza extracts. The elution was joined with DPPH solution (0.3 mL/min) and reacted in the PEEK coil, and then flew into the detector. The anti-oxidation components in S. miltiorrhiza extracts were quickly screened by comparing the chromatograms at 517 nm and 254 nm. Results: The UPLC technology was adopted to realize the efficient separation of samples at low volume flow rate, which avoided the baseline noise caused by high volume flow rate, significantly improved the resolution and sensitivity of the online screening method, and reduced the solvent consumption.The results showed that 31 antioxidant active compounds were screened from S. miltiorrhiza extracts, and 11 of them were identified by PAD-ESI-TOF/MS. Activity verification experiments showed that caffeic acid, salvianolic acid B, salvianolic acid A and danshensu had better antioxidant activity. The IC50 value ofcaffeic acid was 29.00 μmol/L, which was significantly better than the positive control (vitamin C). Conclusion: The method is rapid and efficient, which provides methods and technical support for the rapid discovery of antioxidant components from complex natural products.