Mannheimia haemolytica growth and leukotoxin production for vaccine manufacturing: a bioprocess review

Mannheimia haemolytica leukotoxin (LKT) is a known cause of bovine respiratory disease (BRD) which results in severe economic losses in the cattle industry (up to USD 1 billion per year in the USA). Vaccines based on LKT offer the most promising measure to contain BRD outbreaks and are already commercially available. However, insufficient LKT yields, predominantly reflecting a lack of knowledge about the LKT expression process, remain a significant engineering problem and further bioprocess optimization is required to increase process efficiency. Most previous investigations have focused on LKT activity and cell growth, but neither of these parameters defines reliable criteria for the improvement of LKT yields. In this article, we review the most important process conditions and operational parameters (temperature, pH, substrate concentration, dissolved oxygen level, medium composition and the presence of metabolites) from a bioprocess engineering perspective, in order to maximize LKT yields.

Experiências brasileiras com wetlands construídos aplicados ao tratamento de águas residuárias: parâmetros de projeto para sistemas horizontais / Brazilian experiences with constructed wetlands applied to wastewater treatment: design parameters for horizontal systems

A utilização no Brasil de sistemas tipo wetlands construídos no tratamento de águas residuárias iniciou no ano de 1980, intensificando-se a partir do ano 2000. O objetivo deste trabalho é apresentar um estado da arte da aplicação no Brasil dos wetlands construídos de escoamento subsuperficial de fluxo horizontal (WCFH), identificando parâmetros de projeto e dados operacionais. Aborda-se, inicialmente, uma classificação para os wetlands construídos, seguido dos parâmetros de projeto e operação dos WCFH, destacando os diferentes critérios de dimensionamento, tais como os modelos oriundos da cinética de primeira ordem aplicável aos reatores tipo pistão, a relação de área per capita, o carregamento orgânico superficial e a taxa hidráulica, bem como a composição das unidades de tratamento e as macrófitas empregadas.

The implementation of constructed wetlands for wastewater treatment in Brazil has started in the eighties, being intensified since the year 2000. The aim of this study is to present the state of art of implementation of horizontal sub-superficial flow constructed wetlands (HFCW) in Brazil, focusing on the identification of design parameters and operational data. Initially a classification for constructed wetlands is given, followed by design parameters and operational conditions for HFCW, highlighting different project criteria, e.g. models from first order kinetics for tubular piston reactors, the relation of superficial area per person, the organic superficial loading and the hydraulic loading, as well as the composition of units and macrophytes utilised.