RESUMEN
Objective: To study the effect of micronutrient fertilizer ratio on the growth and yield of aconite daughter root (the processed daughter root of Aconitum carmichaelii). Methods: Taking A. carmichaelii (Ranunculaceae) as test material, to study the growth and yield of aconite daughter root under the various ratio of Zn, B, Fe, and Mn by the uniform design. Results: To promote the growth and improve the yield of aconite daughter root, the optimum ratio of fertilizer Zn-B-Fe-Mn was 4:1:1:2. Under this ratio, the size of the tuber, the number of the fibrous roots, the length of fibrous roots, the volume of the sub-root, and the dry weight of the tuber, the fibrous roots, and the mother root were significantly or very significantly higher than those under the other fertilizer ratio, while the plant height and the dry weight of leaf were lower than those in the control treatment, with the weight of fibrous roots and the mother roots increased by 24.5% and 25.9% compared with those in the control treatment. The results also showed that there existed negative correlation between the plant height and the yield of per plant, while the dry weight of the fibrous roots and the mother roots showed significantly positive correlation with the yield of per plant. Conclusion: The above mentioned ratio of Zn-B-Fe-Mn as 4:1:1:2 is the best, since it could promote the growth and improve the yield of aconite daughter root more than the other treatment.
RESUMEN
Determinou-se a razão ótima de espermatozóides por ovócitos da piabanha Brycon insignis, utilizando-se dois machos e duas fêmeas da espécie, submetidos ao procedimento de desova induzida. Os gametas foram extrusados manualmente 200 horas-grau após a aplicação do extrato bruto de hipófise. Os ovócitos foram misturados e deste pool retiraram-se amostras com 2g de ovócitos (701 ovócitos/g). O sêmen do pool foi diluído em solução de NaCL 1,2 por cento de tal forma que, após a adição de 1ml do sêmen diluído aos ovócitos, fossem obtidas as seguintes razões espermatozóides por ovócitos em cada tratamento: T1=86.662, T2=173.324, T3=259.986, T4=346.648 e T5=433.310. A taxa de fertilização do sêmen não diluído usado como controle foi de 65,3 por cento. Após ativação espermática com NaHCO3 1 por cento e fecundação, os ovos foram transferidos para as incubadoras e nelas foram observadas as seguintes percentagens de fertilização em relação à do grupo-controle: T1=35,7 por cento, T2=53,1 por cento, T3=79,1 por cento, T4=93,4 por cento e T5=87,8 por cento. A percentagem de fertilização (em relação ao controle) aumentou de forma linear, segundo a equação de regressão: Y=15,55+0,0002297X (P<0,01; R²=0,98), até a proporção de 314.481 espermatozóides por ovócitos, após o que a taxa de fertilização se estabilizou no limite de 88 por cento
The optimum spermatozoa:oocyte ratio of piabanha Brycon insignis was studied. Two males and two females were induced to spawn, and the gametes were stripped after 200 hourgrades starting from the application of carp pituitary gland. Oocytes from two females were mixed, and samples of 2g (701 oocytes/g) were collected from the obtained pool and placed into plastic cups. The semen from the two males, after mixed to compose a pool, was diluted in NaCl solution (1.2 percent) so that, after the addition of 1ml of diluted sperm into the oocytes, the following spermatozoa:oocyte ratios (tri-replicated) were obtained: T1=86,662, T2=173,324, T3=259,986, T4=346,648 and T5=433,310. After the activation using 1 percent NaHCO3 and fertilization, the eggs were transferred to incubators, and observed the percentages of fertilization relative to the control. T1=35.7 percent, T2=53.1 percent, T3=79.1 percent, T4=93.4 percent and T5=87.8 percent. Undiluted semen was used as "control", which showed 65 percent of fertilization. The percentage of fertilization (relative to the control) increased linearly according to the regression Y= 15.55 + 0.0002297X (P<0.01; R²=0.98), until the proportion of 314,481 spermatozoa per oocyte, and, from this point, the fertilization rate maintained at 88 percent