RESUMEN
Objective: To study the chemical constituents of flavonoids from Glycyrrhizae Radix et Rhizoma. Methods: The compounds were isolated and purified by column chromatography over HP-20 macroporous resin, silica gel, Sephadex LH-20, and preparative RP-HPLC. Their structures were elucidated by physicochemical properties and spectral analyses. Results: Ten flavonoids were isolated and identified as 4’,6,7-trihydroxy-2’-methoxyl-chalcone (1), 3’,4’,5,7-tetrahydroxy-8-(3-hydroxy-3- methylbutyl)-isoflavone (2), isoliquiritigenin (3), isoliquiritin (4), echinatin (5), orobol (6), ononin (7), 2(S)-3’,5’,7-trihydroxy- flavanone (8), 2(S)-naringenin-4’-O-β-D-glucopyranoside (9), and 4’,7-dihydroxyflavone (10). Conclusion: Compounds 1 and 2 are new compounds named isolicochalcone B and licoisoflavone G, while compound 9 is isolated from the genus for the first time.
RESUMEN
Objective To isolate and identify 3 flavonoids (taxifolin, orobol and quercetin) from Cudrania tricuspidata, and develop a method for determining 3 flavonoid constituents in Cudrania tricuspidata. Methods Three flavonoids was isolated from ethanol extract of Cudrania tricuspidata by chromatography, and its structure was identified by nuclear magnetic resonance. The analysis was conducted on an Aglient C18 column (4.6 mm ×250 mm, 5 μm) eluted with 1％ acetic acid and methanol as mobile phases in gradient mode. The flow rate was 1 ml/min and the detection wavelength was set at 310 nm. The column temperature was 25 ℃. Results Taxifolin, orobol and quercetin were isolated from ethanol extract of Cudrania tricuspidata by chromatography. The content of taxifolin, orobol and quercetin were 0.850 mg/g, 0.518 mg/g, 0.103 mg/g. Conclusion The method can be used for the quality control of Cudrania tricuspidata as a reference.