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[ ABSTRACT] AIM:To detect the differentially expressed genes associated with ovarian serous cystadenocarcino -ma ( OV) by microarray and to analyze the participated signaling pathway .METHODS:We analyzed 16 datasets of Affy-metrix GeneChip Human Exon 1.0 ST Arrays from The Cancer Genome Atlas ( TCGA) , including 8 OV and 8 normal ovary samples.The function of differential genes was determined by pathway and gene ontology ( GO) analysis.The probable functions of the key genes were predicted according to intergenic signal transduction network .RESULTS:The 1 144 genes were identified as distinctively expressed in OV (P<0.05), 747 of which were up-regulated and 397 were down-regula-ted.The GO analysis results showed that the altered genes were involved in 362 up-regulated and 160 down-regulated sig-nificant functions (P<0.05) related to cell cycle, DNA replication, cell proliferation, cell apoptosis, cell adhesion, etc. The pathways of the different genes were involved in the 59 enrichment-related pathways (P<0.05), 45 of which were up-regulated and 14 were down-regulated.Among the 59 pathways, cell cycle, P53 signaling pathway, DNA replication, path-ways in cancer, PI3K-Akt signaling pathway, ECM-receptor signaling pathway, cell adhesion molecules and cell apoptosis were related to tumor genesis , development and metastasis .As a result, 229 genes with significant functions and pathways in GO and pathway analysis were selected to construct signal transduction network ( Signal-Net), 4 of which, CDK1, PLK1, MCM3 and PGK1, were found to play key roles in OV signal regulation network .CONCLUSION: The OV shows abundant differentially expressed genes that play key roles in cancer-related signal pathways .
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So-called sclerosing hemangioma of the lung is a rare benign neoplasm which usually presents with a coin lesion detected through routine chest X-ray. We report two cases showing characteristic cytologic appearances which have been rarely reported. Both cases were young females with coin lesions in the lung. Fine needle aspiration of each case revealed unique but some different cytologic features. Case 1 showed bland-looking polygonal epithelial cells resembling bronchioloalveolar cells having pre- dominantly papillary configurations with loosely arranged solid sheets or isolated cells. Cytoplasms were plump, cyanophilic, and finely granular, with eccentric nuclei, The nuclei were usually monotonous, round-to-ovoid, and vesicular with a small but conspicuous nucleolus. In comparison to case 1, case 2 revealed largely loose pavement-like solid sheets or clusters rather than papillary patterns in the hemorrhagic background. The size of tumor cells were a little smaller than that of case 1. Bronchioloalveolar carcinoma and papillary adenocarcinoma of metastatic origin were considered to be one of the important differential diagnoses with these cytologic features. Histologically, both cases exhibited findings compatible with so-called sclerosing hemangioma of the lung.