Efficient validated method of UPLC-MS/MS to determine curcumin in rat plasma and ovarium

Curcumin, a lipophilic polyphenol derived from the roots of Curcuma longa. Recently, it has been widely investigatedas a therapeutic agent for cancer. Thus, there is a growing interest in measuring curcumin concentrations in theplasma and other target tissues in relevant animal models. We developed and validated a simple, fast, and reliablemethod for quantifying curcumin in biological matrices by Ultra Performance Liquid Chromatography (UPLC)-MassSpectrometry (MS)/MS. The liquid chromatography system is using rapid separation on Acquity UPLC®BEH C18with gradient mobile phase contained formic acid and acetonitrile. Prior to detection, curcumin and internal standard(IS) were ionized using electrospray ionization positive source and the ions were monitored at m/z 369 → 177 and 260→ 183 for curcumin and IS, respectively. The calibration curve was linear (r ≥ 0.99) over the concentration range of1–50 ng/ml and 1–30 ng/ml for rat plasma and for ovary homogenate, respectively. The lower limit of quantificationwas 1 ng/ml. The mean accuracy ranged from 98.9% to 103.2% and 98% to 108.9%, while the coefficient of variation(CV) values of precision in rat plasma were below 11.92% and 10.47% for within and between run, respectively. Inrat ovary homogenate, the mean concentration and CV of within run accuracy and precision were 95.53%–109.78%and 3.34%–9.14%, respectively. The developed method was used to quantify curcumin in rat plasma and ovary afteran oral gavage. In conclusion, the developed and validated method should be useful for quantification of curcuminaccurately and precisely in plasma and target organs from relevant animal models of human diseases.

N-acetylcysteine improves morphologic and functional aspects of ovarian grafts in rats

PURPOSE: To evaluate morphological and functional aspects of the ovarian graft in transplanted rats treated with NAC. METHODS: Female Wistar rats, virgin, 3 to 4 months old, weighing 200-250 grams were used in experiments. The rats have been kept in proper sanitary conditions, receiving food and water ad libitum. Five groups (n=10, each) were constituted: 4 groups treated subcutaneously with NAC, at doses of 150, 300, 600 and 1200 mg/kg (NAC150, NAC300, NAC600 and NAC1200, respectively), one hour of before the ovarian transplantation and control group (GTx) - treated with physiological solution and submitted to ovarian transplantation. The rats were anesthetized and submitted to autologous left ovarian transplantation, without anastomosis in retroperitoneum, and contralateral oophorectomy. During follow-up of 4 or 15 days, the estrous cycle was evaluated by vaginal smears to determine cycle regularity. At the end of 4th or 15th days, rats were re-anesthetized and blood and graft were obtained to estradiol analysis and morphological assessment. Data were analysed by One Way Analysis of Variance (ANOVA) or ANOVA on ranks complemented by Student-Newman-Keuls test. RESULTS: At 4th day, viable follicles in the graft did not altered by NAC treatments. The NAC300 and NAC600 groups showed increasing in follicle atresia (p=0.012) compared to GTx and NAC1200 group. At 15th day, 50% of GTx, NAC150, and NAC300 rats showed regular oestrous cycle; 83% of NAC600 and 100% of NAC1200 rats returned to regular cycle. NAC1200 group showed increasing in primordial follicle compared to GTx, NAC150 or NAC300 (p=0.011). NAC did not interfere in estradiol levels after 4 or 15 days of transplantation. CONCLUSION: In autologous ovarian transplantation, high dose of NAC promotes graft viability with recovery of estrous cycle. .

Migración de un dispositivo intrauterino al ovario / Migration of a intrauterine device to ovarium

Los dispositivos intrauterinos constituyen el método anticonceptivo más empleado en países en vías de desarrollo. No exentos de complicaciones y, al igual que cualquier otro cuerpo extraño implantado en el organismo, puede migrar. Se realiza la presentación de una paciente en la que se diagnostica la migración de un DIU al ovario, una de las localizaciones más infrecuentes, y su exitosa remoción por cirugía videoasistida. Se recomienda este método como proceder de elección para la extracción de estos dispositivos translocados a la cavidad abdominal en los casos no complicados

The intrauterine devices (IUD) are the more used contraceptive method in developing countries. Not exempt of complications and just like other foreign body inserted in the organism, may migrate. This the case of a patient diagnosed with migration DIU to ovarium, one of the more frequent locations and its successful removal by means of video-assisted surgery. Authors recommended this method as a choice procedure to removal of theses translocated devices to abdominal cavity in non-complicated cases

Observation effect for cancer factor exhausted during chemotherapy intermittent for drinking, food and oxygenic sport interference to patients suffered from ovarium cancer / 中国基层医药

Objective To explore cancer factor exhausted during chemotherapy intermittent for drinking, food and oxygenic sport interference to patients suffered from ovarium cancer. Method 92 patients suffered from ovarium cancer with drinking, food and oxygenic sport interference and taking self-comparison method. Simple tired Assessment Form, European cancer treatment and research origination questionnaire (EORTC-QLQ-C30) are as-sessed for the patients suffered from cancer tied and life quality situation before interference and after interference of 30 d so as to observe the tired relieving degree and life quality of the patients before and after interference. Results Using nursing interference and then the patients relive their tiredness greatly to have statistical significance (P <0.01). The comparison before and after interference and the life quality has statistic significance(P <0.01). Con-clusion The drinking, food and oxygenic sport interference can relieve the fired degree of the patients suffered from ovarium cancer effectively to improve the life quality of the patients.

Tummor of ovarium in children and pubescent girls

Research on 31 patients who lower than 18 years old with tumor of ovarium at The protect mother and newborn Hospital from 1987 to 1993. Result: Tumor of ovarium at the age is very rare. Rate of malignant tumor is high and most of the tumor has origin from seed cell of ovarium. Majority patients was found slowly when the tumor at the end of period. Clinical symptoms is poor. Need to cut the tumor and trying to maintain normal part of ovarium. For malignant tumor, treatment base on organization of tumor cell. When surgery, need to cold cut organ of ovarium to have better activity. After surgery, can combine with chemical or ray X therapies as well as immune therapy