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ObjectiveTo study androgen excess within ovarian granulosa cells of PCOS patients in vitro,and the partial improvement by using astragalus polysaccharides.MethodsOvarian granulosa cells from the PCOS patients during IVF / ICSI - ET were isolated and cultured in vitro,then it was treated by different concentrations of astragalus polysaccharides.The levels of estradiol,progesterone and testosterone were determined.ResultsAndrogen secretion within ovarian granulosa cells from PCOS patients was significantly increased [ ( 0.4716 ± 0.03 ) nmol/L,( 0.23 ± 0.0162 ) nmol/L,P ＜ 0.05 ].The levels of estradiol and progesterone were not significantly different( P ＞ 0.05 ).Different concentrations of astragalus polysaccharides may result from the alleviation of androgen secretion which was commonly found in PCOS treatment (concentration was 1000 μg/ml,100 μg/ml,50 μg/ml,P ＜ 0.01 ;concentration was 500 μg/ml,P =0.002 ; concentration was 200 μg/ml,P =0.003).ConclusionsAstragalus polysaccharides inhibit the secretion of testosterone in granulosa cells of PCOS patients during in vitro,and it may be an alternative treatment for PCOS.
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Objective To evaluate the clinical value of CHO-hTSHR cells in detecting thyroid stimulating antibody (TSAb). Methods The cAMP production and TSAb activity were measured and cal-culated by stimulating CHO-hTSHR cell line with IgGs of normal control group and Graves" disease ( GD )group. TSAb positive standard was set to more than the mean + 2SD of TSAb activities in control subjects.The positive percentage of TSAb activity in GD group was calculated. Results The cAMP production and TSAb activities of GD group were higher than those of normal group[( 353. 65±126. 34 ) pmol/L vs (237.21±77. 15)pmol/L, ( 149. 08±53. 26)% vs ( 100±32. 52)%, P <0. 05] . The value that higher than 165% was set to be positive for TSAb. The positive percentage of TSAb in GD group was 50% ( 14/28). Conclusion CHO-hTSHR cell line constructed by our group is suitable for detecting TSAb activity in the sera of patients with GD.