RESUMEN
Aim To study whether the genes S100A8 and S100A9 are related to the functional regulation of oviduct by estrogen, and to explore their possible effects on fallopian tubes. Methods The basic expression and distribution of S100A8 and S100A9 in ampullary oviduct tissues of healthy sheep in diestrum were verified by immunohistochemistry. The expressions of S100A8 and S100A9 (mRNA and protein) in oviduct epithelial cells were detected by q-PCR and immunofluorescence, the cells were treated by E2 at different time points and different concentrations. Results S100A8 and S100A9 were highly expressed in mucosal epithelium and glandular epithelium of sheep uterine tubes during the diestrum period, and in blood vessels as well. The expression of S100A8 and S100A9 in tubal epithelial cells changed dynamically at different time ponits under the action of high concentration of E2 in vitro, and reached the peak 6 hours after E2 treatment. At this time, different concentrations of E2 significantly induced the high expression of S100A8 and S100A9, but the expressions of S100A8 and S100A9 were the highest at the concentrations of 10-7 mol • L-1 and 10-8 mol • L-1 , and there was no significant difference between the two groups. Conclusions S100A8 and S100A9 in oviduct epithelial cells are regulated by estrogen. Under the regulation of high concentration estrogen, the high expression of SI00A8 and S100A9 may be related to the natural defense of reproductive tract during mating, and may be involved in the transport of eggs in fallopian tube.
RESUMEN
Calcium blockers verapamil ( VER ) and nifedipine ( NIF ) appa-rentely inhibited the spontaneous activity of isolated rat oviduct. The 5 min contractile curve aera determined by integrator,after eithet VER or NIF effected, was smaller than that before,with dose dependence.In rats pregnanant for 3d VER and NIF accelerated ovum transport through oviduct. The number of ovum recovered from uterus in test group was more than that in control group. Paired experiments showed VER and NIF having no anti-implantation action.
RESUMEN
The effects of DL-tetrahydropalmatine andsinomemine on the activity of oviduct smooth mus-cle and ovum transport in rabbits are reported inthis paper. The results indicate that the two a-gents apparently inhibit the contractile activity ofoviduct isthmic smooth muscle of rabbit in vitroand decrease the frequency, amplitude, tensionand curve areas of the contraction. The contractileffect induced by noradrenaline is also inhibited re-markbly by them. In addition, DL-THP and Sino.can delay ovum transport in rabbit oviduct after ivHCG 48 h. It is suggested that the inhibition ofactivity of oviduct smooth muscle may be one ofthe important causes of delaying egg transportcaused by the two agents.
RESUMEN
Progesterone receptors were determined in the cytosol from the ampulla, ampullaryisthmic junction and isthmus of rabbit fallopian tube and uterus of estrus and pregnant rabbits. The receptor levels when compared among its various anatomical segments, were the same in ampulla, isthums and uterus but maximum in ampullary-isthmic junction. Significant differences were observed in mated animals at 14, 24, 34, 48, 70 and 144 h after coitus. The receptor concentrations in portions of the fallopian tube showed no significant change between 14 and 24 h after coitus, except for a decrease in ampullary-isthmic junction at 24 h. At 34 h the concentration of receptor further decreased in all parts of the tube. At 48 and 70 h after coitus, receptor concentrations decreased gradually in ampulla and ampullary-isthmic junction, while isthmus showed a gradual increase. At 144 h, the receptor concentration showed no further change in ampulla and ampullary-isthmic junction; however, isthmus showed a decline. The uterine receptor concentration declined steadily from estrus till 70 h after coitus, however, it was increased at 144 h. The dissociation constant (Kd) of cytosol receptor in all the tissues at estrus and during early pregnancy was found similar. The implications of these changes in relation to the normal ovum transport have been correlated in this paper.