RESUMEN
OBJECTIVE: To investigate the chemical constituents from the leaves of Cyclocarya paliurus. METHODS: Chemical constituents were isolated and purified by column chromatography with silicagel, Sephadex LH - 20, and semi-preparative HPLC. RESULTS: Eleven compounds were isolated from the ethyl acetate extracts of the leaves of Cyclocarya paliurus. Their structures were elucidated as hirsutrin (1), contigoside B (2), kaempferol (3), sterubin (4), syringic acid (5), vanillic acid (6), 2-hydroxy-5-ethoxybenzoic acid (7), 2-methoxy-4-hydroxycinnamic acid (8), cis-p-hydroxycinnamic acid (9), p-hydroxybenzonic acid (10), and phydroxybenzaldehyde (11). CONCLUSION: All the compounds except 3 and 6 are obtained from Cyclocarya paliurus for the first time.
RESUMEN
Objective To qualitatively analyze the chemical constituents in rats after intragastric administration of estrogenically active ethanol extract of Cuscuta chinensis. Methods The high performance liquid chromatography quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF MS/MS) was used to identify the prototypes and metabolites in rat urine. Results Twelve chemical constituents were identified in the drug-containing urine, including six prototypes and six metabolites. The prototypes are kaemoferol-3-β-D-glucuronide, 6-O-(E)-p-coumaroyl-β-D-fructofuranosyl-(2→1)-α-D-glucopyranoside, aempferol-7-rhamnoside, chlorogenic acid, and apigenin. The metabolites are p-hydroxycinnamic acid, p-hydroxyphenylpropionic acid, isorhamnetin, kaempferol-3-O-glucoside, acetyl caffeic acid, and quercetin sulfate. Conclusion The method of HPLC-Q/TOF MS/MS is simple and rapid for the analysis of prototype components and metabolites in rats urine after oral administration of ethanol extract of C. chinensis, providing the basis for further clarification of the estrogen material basis of C. chinensis.
RESUMEN
OBJECTIVE: To study the chemical constituents of Whole Plant to the whole plant of Veronicastrum axillare(Sieb. et Zucc.)Yamazaki. METHODS: The compounds were isolated and purified by various chromatographic techniques, and their structures were identified on the basis of physicochemical properties and spectral analysis. RESULTS: Twenty-three compounds were identified as 5-hydroxy-7, 4'-dimethoxyflavone(1), acacetin(2), 5, 7, 4'-trihydroxy-8-methoxyflavone(3), 5, 7, 3'-trihydroxy-4'-methoxyflavone(4), luteolin(5), oleic acid(6), syringaresinol(7), glyceryl ferulate(8), cinnamic acid(9), p-hydroxycinnamic acid(10), caffic acid(11), ferulic acid(12), 3, 4-dimethoxycinnamic acid(13), p-hydroxybenzoic acid(14), protocatechuic acid(15), vanillic acid(16), isovanillic acid(17), protocatechuic aldehyde(18), hydroquinone(19), 1, 2, 4-benzenetriol(20), D-mannitol(21), sucrose(22), and β-sitosterol(23). CONCLUSION: All the compounds are isolated from this plant for the first time except for compounds 2, 5, 19, 21 and 23, and from the genus Veronicastrum for the first time except for compounds 2, 5, 6, 12, 13, 19, 21 and 23. The 13C-NMR data of compound 8 is reported for the first time.