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Aim To explore whether isopropyl3-(3, 4-dihydroxyphenyl) -2-hydroxypropanoate (IDHP) could inhibit fat accumulation in liver cells by improving mitochondrial function, and alleviate the symptom of excessive fat accumulation in patients with NAFLD. Methods Cell steatosis model was established by inducing hepatocyte fat accumulation using palmitic acid and oleic acid (PA: OA molar ratio =1
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Objective Here we intend to find out activity of EC-PAOA on the proliferation of normal endometrial and eutopic endometrial cells in vitro.Methods Normal human endometrium and eutopic human endometriosis were used,stromal and epihelisl cells were separated by selective enzymatic digestion method,Cells types and purities of cells were identified through immunohistochemistry result;The isolated cells were primarily cultured,while the other pat cultured with different concentrations of EC-PAOA(10,100,1000ug/ml ) for 24,48 and 72h,The effect of cultured cells from nomal endometrium and eutopic endometriosis were observed by MTT assay.Results EC-PAOA was observed no effect on normal endometrium, in the contrast, EC-PAOA was found to anti proliferation of eutopic endometrial cells and was most notable to anti proliferation of eutopic endometrial cells(P
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Objective:To investigate EB PAOA's effects on the level of cytokines produced by the mice transplanted with S 180 cells and induction actions for apoptosis of colon cancer CL 187 cells so as to probe into EB PAOA's antineoplastic active mechanism preliminarily Methods:MTT assay was used to detect EB PAOA's effects on the level of cytokines,including IL 2?IFN ??IL 1 and TNF ?,produced by the mice transplanted with S 180 cells Then, human colon cancer CL 187 cell strains were cultured in vitro and MTT assay was adopted to determine the inhibition rate to CL 187 cells Apoptotic cells were observed and confirmed with an electronmicrograph Apoptotic rate were then detected through a flow cytometer Results:The ability of the mice transplanted with S 180 cells to produce IL 2?IL 1 and TNF ? after treated with EB PAOA was strengthened EB PAOA can inhibit the proliferation of CL 187 cells Large number of apoptotic cells were found under the electronmicrograph There was an apoptotic peak appeared in DNA histogram of the flow cytometer The apoptotic rate was of time dependent.Conclusion:The antineoplastic mechanism of EB PAOA may be realized by stimulating the splenocytes of the mice transplanted with S 180 cells to secrete IL 2 and macrophages to secrete IL 1 and TNF ? to strengthen cellular immune functions and induce the apoptosis of tumor cells directly