When the savior becomes a demon: Silicon oil synechia-induced glaucoma

Background: Silicon oil is an important adjunct for achieving internal tamponade in the treatment of retinal detachment. Silicone oil tamponade often leads to narrowing of the angle and development of adhesions between the iris and anterior chamber angle structures, with consequential elevation of the intraocular pressure. The video showcases the management of these challenging scenarios. Purpose: To highlight the management of early synechial closures due to silicon oil tamponade. Synopsis: The video highlights the management of early synechial closure following silicon oil tamponade. Surgical pupilloplasty has been demonstrated to break the peripheral anterior synechias on intraoperative gonioscopy as well as on anterior segment optical coherence tomography (AS?OCT). Performing pupilloplasty in the early phase of development of peripheral anterior synechias (PAS) helps to break the existing synechias and prevent angle closure and sequential deterioration of vision. Highlights: Surgical pupilloplasty helps to relieve the post silicon oil?induced secondary angle closure glaucoma by breaking the peripheral anterior synechias and significantly opening the anterior chamber angles.

A Malignant Granular Cell Tumour with an Unusual Presentation: A Rare Case Report

A granular cell tumour is a tumour of neural origin and is mostly benign and rarely malignant and commonly found in the skin, oral cavity or digestive tract. This report describes a case of a 23 - year - old female with a palpable painless mass on the back near the left axilla with progressive growth. While her laboratory tests and radiological were within normal limits histopathological examination revealed round to polygonal PAS positive atypical cells with large vesicular nuclei and coarsely granular cytoplasm along with foci of spindling an d necrosis which on IHC were S - 100 and CD68 positive and negative for cytokeratin, desmin, EMA. Thus, rendering a diagnosis of a malignant granular tumour of primary origin. This case highlights the importance of accurate histopathological examination to r ecognise and further categorization of Granular cell tumours along with clinical and radiological correlation .

Simulação de esquizofrenia no Teste de Rorschach (R-PAS) / Schizophrenia simulation in the Rorschach Test (R-PAS) / Simulación de esquizofrenia en el Teste Rorschach (R-PAS)

Este trabalho teve como objetivo comparar o desempenho no Teste de Rorschach (R-PAS), de pessoas instruídas sobre os sintomas da esquizofrenia e solicitadas a responder ao Rorschach, tentando se passar por um paciente com esse diagnóstico (n = 40), com o de pacientes diagnosticados com esquizofrenia (n = 35). Os participantes responderam ao Teste de Rorschach (R-PAS), a Magical Ideation Scale (MIS) e ao Inventory of Problems-29 (IOP-29) tentando simular esquizofrenia. Os resultados das comparações entre os grupos evidenciaram diferenças estatisticamente significativas para as variáveis (Hd), An, FQo, FQu, FQ-, P, M, PEC, WSumCog, MAH, GHR e Complexity. Encontrou-se também associação positiva e de forte magnitude entre os escores obtidos no IOP-29 e os escores brutos da MIS (r = 0,73, p = 0,0001). Os achados evidenciaram que, mesmo diante das tentativas de distorção das respostas ao teste, simuladores não conseguem distanciar da própria precisão perceptiva. (AU)

This study compared the performance in the Rorschach Test (R-PAS) of people informed about the symptoms of schizophrenia and asked to respond to the Rorschach trying to impersonate a patient with this diagnosis (n = 40) with that of patients diagnosed with schizophrenia (n = 35). Participants responded to the Rorschach Test (R-PAS), the Magical Ideation Scale (MIS) and the Inventory of Problems-29 (IOP-29) trying to simulate schizophrenia. The results of the comparisons between the groups showed statistically significant differences for the variables (Hd), An, FQo, CFQ, CF-, P, M, PEC, WSumCog, MAH, GHR and Complexity. A positive and strong association was also found between the scores obtained in the IOP-29 and the gross scores of the MIS (r=.73, p=.0001). The findings show that even faced with attempts to distort the responses to the test, simulators cannot distance themselves from their perceptual accuracy. (AU)

Este estudio tuvo como objetivo comparar el rendimiento en el Test de Rorschach (R-PAS) de personas educadas sobre los síntomas de la esquizofrenia y solicitadas a responder al Rorschach, tratando de pasar por un paciente con este diagnóstico (n = 40) con el de los pacientes diagnosticados con esquizofrenia (n = 35). Los participantes respondieron al Test de Rorschach (R-PAS), a la Magical Ideation Scale (MIS) y al Inventory of Problems-29 (IOP-29) intentando simular la esquizofrenia. Los resultados de las comparaciones entre los grupos mostraron diferencias estadísticamente significativas para las variables (Hd), An, FQo, CFQ, CF-, P, M, PEC, WSumCog, MAH, GHR y Complexity. También se encontró una asociación de magnitud positiva y fuerte entre los scores obtenidos en la IOP-29 y los scores brutos del MIS (r = 0,73, p = 0,0001). Los hallazgos muestran que, incluso frente a los intentos de distorsionar las respuestas del test, los simuladores no son capaces de distanciarse de su precisión perceptiva. (AU)

Determination and Risk Assessment of Six Hepatotoxic Pyrrolizidine Alkaloids in Verbenae Herba / 中国实验方剂学杂志

Objective:To establish ultra-performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS） for simultaneous determination of six hepatotoxic pyrrolizidine alkaloids in Verbenae Herba， and to carry out preliminary risk assessment according to the research results. Method:An ACQUITY UPLC HSS T3 column （2.1 mm×100 mm， 1.8 μm） was used for analysis with 0.05% formic acid and 2.5 mmol·L^-1 ammonium formate in water （A）-0.05% formic acid and 2.5 mmol·L^-1 ammonium formate in acetonitrile （B） as mobile phase for gradient elution （0-12 min， 3%-8%B； 12-25 min， 8%-15%B； 25-26 min， 15%-3%B； 26-30 min， 3%B）， the flow rate was 0.3 mL·min^-1， the column temperature was 40 ℃， and the injection volume was 1 μL. MS system was operated by electrospray ionization （ESI） in the positive ion mode with multiple reaction monitoring mode. MS parameters of triple quadrupole and six analytes were optimized for qualitative and quantitative analysis. According to the determination results， the risk assessment was carried out by using margin of exposure （MOE） combined with transfer rate of hot water extraction. Result:Based on the instrument precision， linear range， repeatability， stability， recovery and other methodological validations， the results were in conformity with relevant standards of quantitative analysis. The linear ranges of intermedine， lycopsamine， intermedine <italic>N</italic>-oxide， lycopsamine<italic> N</italic>-oxide， echimidine<italic> N</italic>-oxide and echimidine were good （<italic>r</italic>≥0.999 0） between peak area and mass concentration in the ranges of 0.984-49.20， 0.994-49.70， 1.012-50.60， 1.032-51.60， 1.004-50.20， 1.016-50.80 µg·L^-1， respectively. The average recoveries of these six analytes were 87.2%-94.2% with relative standard deviation （RSD）<4.0%. Their MOE values were >10 000. Conclusion:The UPLC-MS/MS established in this study is stable and feasible， which can provide scientific basis for the quality control and safety evaluation of hepatotoxic pyrrolizidine alkaloids in Verbenae Herba.

Anatomía general, histología y morfometría del sistema digestivo del pez Pterophyllum scalare (Perciformes: Cichlidae)

Introducción: El pez escalar (Pterophyllum scalare) es una de las principales especies de peces ornamentales comercializados de Sur América. Objetivo: Describir histológica y morfométricamente el tracto digestivo del pez tropical P. scalare. Métodos: Se utilizaron 19 individuos adultos, y se calculó el coeficiente intestinal. Se realizó una descripción macroscópica e histológica del esófago, estómago e intestino (región anterior parte a, anterior parte b, media y posterior). Las muestras fueron procesadas en parafina, se obtuvieron cortes de 5 µm, que fueron teñidos con H&E y P.A.S. Se realizó conteo de células P.A.S +. Para la morfometría se digitalizaron los cortes y se midió el área y perímetro del lumen, y el área tisular para cada región. Resultados: P. scalare presenta un esófago corto, un estómago en forma de saco ciego poco muscular junto a un intestino con una longitud similar a su tamaño, coeficiente intestinal (1.66 +/- 0.52). Se identificaron cuatro capas constitutivas: mucosa, submucosa, muscular y en algunas regiones serosa. El esófago se destacó por ser la región con mayor número de células caliciformes P.A.S. + y mayor área tisular. En cuanto al área y perímetro luminar, la región anterior parte a del intestino fue la de mayor valor. Conclusiones: A partir del coeficiente intestinal (1.66 +/- 0.52), y las características histológicas y morfométricas de los órganos evaluados, se puede decir que P. scalare presenta un hábito alimenticio omnívoro con preferencia de presas blandas.

Introduction: Ornamental Angel fish (Pterophyllum scalare) is one of the main commercialized species from South America, with an important number of national and international dealers. Objective: To make a histological and morphometric description of P. scalare digestive tract. Methods: Nineteen adult individuals were used. Intestinal coefficient values were obtained, and a macroscopic and microscopic description of esophagus, stomach, and intestine (anterior, middle and posterior) was carried out. Samples were processed for paraffin embedding, and 5 µm sections were stained with H&E and P.A.S. Positive P.A.S. cells were counted. Lumen area and tissue area were measured in representative digitalized sections from each region. Results: P. scalare exhibited a short esophagus, followed by a blind bag shaped, scarcely muscular stomach, and a short intestine (intestinal coefficient 1.66 +/- 0.52). Four constitutive tissue layers were identified: mucosa, submucosa, muscular and in some areas also a serosa layer. Esophagus exhibit abundant globet P.A.S. + cells and shows the larger tisular area was observed. Luminal area and perimeter are larger in anterior intestinal region. Conclusions: The intestinal coefficient value (1.66 +/- 0.52), and the histological and morphometric characteristics of the evaluated organs, suggest that P. scalare is omnivorous with soft prey preferences.

Overexpression of bHLH domain of HIF-1 failed to inhibit the HIF-1 transcriptional activity in hypoxia

BACKGROUND: Hypoxia inducible factor-1 (HIF-1) is considered as the most activated transcriptional factor in response to low oxygen level or hypoxia. HIF-1 binds the hypoxia response element (HRE) sequence in the promoter of different genes, mainly through the bHLH domain and activates the transcription of genes, especially those involved in angiogenesis and EMT. Considering the critical role of bHLH in binding HIF-1 to the HRE sequence, we hypothesized that bHLH could be a promising candidate to be targeted in hypoxia condition. METHODS: We inserted an inhibitory bHLH (ibHLH) domain in a pIRES2-EGFP vector and transfected HEK293T cells with either the control vector or the designed construct. The ibHLH domain consisted of bHLH domains of both HIF-1a and Arnt, capable of competing with HIF-1 in binding to HRE sequences. The transfected cells were then treated with 200 µM of cobalt chloride (CoCl2) for 48 h to induce hypoxia. Real-time PCR and western blot were performed to evaluate the effect of ibHLH on the genes and proteins involved in angiogenesis and EMT. RESULTS: Hypoxia was successfully induced in the HEK293T cell line as the gene expression of VEGF, vimentin, and ß-catenin were significantly increased after treatment of untransfected HEK293T cells with 200 µM CoCl2. The gene expression of VEGF, vimentin, and ß-catenin and protein level of ß-catenin were significantly decreased in the cells transfected with either control or ibHLH vectors in hypoxia. However, ibHLH failed to be effective on these genes and the protein level of ß-catenin, when compared to the control vector. We also observed that overexpression of ibHLH had more inhibitory effect on gene and protein expression of N-cadherin compared to the control vector. However, it was not statistically significant. CONCLUSION: bHLH has been reported to be an important domain involved in the DNA binding activity of HIF. However, we found that targeting this domain is not sufficient to inhibit the endogenous HIF-1 transcriptional activity. Further studies about the function of critical domains of HIF-1 are necessary for developing a specific HIF-1 inhibitor.

Adaptación al español de cuatro instrumentos para evaluar la teoría de la mente en niños y adolescentes / Spanish adaptation of four instruments to asses Theory of Mind in Children and adolescents

Resumen Introducción: La teoría de la mente (ToM) es una de las dimensiones de la Cognición Social más relevantes y de gran interés para la investigación en psicología del desarrollo, psicología cognitiva, neuropsicología y neurociencias sociales, así como en la labor clínica. Esto ha conllevado a la generación de instrumentos validados para la evaluación de esta función; sin embargo, en español son pocos los que hay y aún menos los adaptados para población infantil y adolescente. Objetivo: En el presente trabajo se presenta la adaptación de la Batería de la ToM, el inventario de la ToM, el Test de Paso en Falso y el Test de la Mirada, cuatro instrumentos con tareas clásicas para evaluar la ToM. Método: El proceso de adaptación se hizo en dos fases, una de traducción al español y otra de adaptación cultural. Para verificar los resultados se hizo un pilotaje para todos los instrumentos con 20 participantes en edades entre 4 y 13 años y 20 participantes entre 6 y 17 años. Resultados: Se presentan los cuatro instrumentos adaptados en español con los principales ajustes en cada uno de ellos. Discusión: Se discute sobre la importancia de generar un protocolo de valoración de la ToM haciendo una validación estadística de estos instrumentos.

Abstract Introduction: Theory of Mind (ToM) is one of the most important dimensions of Social Cognition (SC); it is of great interest for research in Development Psychology, Cognitive Psychology, Neuropsychology, and Social Neuroscience as well as for clinical practice. It has stimulated the creation and validation of instruments to asses this dimension as a cognitive process, but there are few instruments like these in Spanish and less to evaluating children and adolescents. Objective: This paper reports the adaptation into Spanish of four instruments to asses ToM: The Theory of Mind Battery, The Theory of Mind Inventory-2, the Reading the Mind in the Eyes and the Faux Pas Test. Method: The adaptation process was made in two steps first, translation and, second, cultural adaptation. To verify the results a pilot testing was carried out in two groups, one with children between 4 and 13 years old and other with children and adolescents between 6 and 17 years old. Results: Instruments adapted into Spanish are presented as results highlighting the main adjustments made to each tool. Discussion: The importance of creating a protocol with statistical validation to asses ToM are discussed.

Study On Comparison Of Nebulization With Salbutamol Alone With Combination Of Salbutamol And Ipratropium Bromide In The Treatment Of Acute Exacerbation Of Bronchial Asthma

Introduction:Asthma is a chronic inflammatory condition of the lung airways resulting in episodic airflow obstruction. This chronic inflammation heightens the airway hyper responsiveness (AHR). Asthma is defined by the history of respiratory symptoms like wheeze, shortness of breath, chest tightness and cough that vary over time and in intensity together with variable expiratory airflow limitation.1 Aims & Objectives: To compare the effectiveness of a combination of inhaled anticholinergics (ipratropium bromide) and beta 2 agonists (salbutamol) compared with beta 2 agonists (salbutamol) alone for the treatment of children with acute exacerbation of asthma. Methods: This is a randomized comparative study conducted in the Department of pediatrics ASRAM Medical College during the period May 2018 to June2019. Total 66 children between the age group of 1-15 years with eligible diagnostic criteria were included in the study. Results: The percentage increase in predicted PEFR is better in Group II with a mean of 24.74 compared to Group I with a mean of 13.35. The p value is highly significant 0.001. The mean PAS at the start of the study is 10.5 in Group I and 10.7 in Group II. The mean PAS at the end of the study is 7.19 in Group I and 5.77 in Group II. The p value is 0.001 and is highly significant. The outcome is better in Group II when compared to Group I with a better decrease in PAS and better increase in percentage predicted PEFR. Conclusion: In the present study it has been proven the repeated doses of Ipratropium bromide combined to Salbutamol is beneficial and it reduces the broncomotor cholinergic tone.

Thrombosis Prediction of a Catheter Pump Based on Simulation Method / 医用生物力学

Objective To investigate a self-designed catheter pump by using computational fluid dynamics (CFD) method, so as to predict its hydraulic performance and risk of thrombosis formation. Methods The thrombosis prediction models proposed by Grigioni and Danny Bluestein were used. The shear stress and exposure time during platelet motion were calculated by CFD method, and parameters of platelet activation state (PAS) were obtained for prediction of thrombogenic performance. Results At the flow rate of 4 L/min and rotating speed of 10 000 r/min, the differential pressure of the pump reached 14.763 kPa and the hydraulic performance was proved to fit the requirement of left ventricular assist device. The PAS values of Grigioni model and Danny Bluestein model were 6.35×10-6 and 7.68×10-4,respectively, both at a very low level, indicating a low possibility of thrombus formation. Conclusions This study investigated the feasibility of thrombosis prediction based on simulation method and the predicted hydraulic performance and thrombosis will provide references for further design optimization.

Granular cell tumor of breast: clinicopathological and biological behavior / 中华内分泌外科杂志

Objective To investigate the clinicopathological and immunohistochemical features,diagnosis and prognosis of granular cell tumor of breast (GCT)and to improve the awareness of the disease.Methods Three cases of GCT were collected;Specimens were fully drawn,microscopic pathologic examinations and immunohistochemistry (SP method)were performed.Results Three cases were female patients aged from 39 to 56 years old (average 46 years).In clinical,a single indolent or indolent mass with a hard texture was located in the breast parenchyma.In pathological,the lump was mainly solid or hard tumor with clear boundary or infiltration,with a mean diameter of 2.1 (1.2-3.0)cm,and grey to yellow sections.Histologically,large cells were round or polygonal in shape.The cytoplasm was abundant and eosinophilic.The boundary of the tumor was clear in one case,and in the other two cases,the boundary was unclear.The nucleus was small and located in the center or ectopic.The cytoplasm was coarse-grained with s-100 staining positive microparticles and PAS reaction positive (anti-digestive enzymes).Immunohistochemistrically,the tumor cells were strongly positive for S-100,CD56,NSE and Vimentin,and negative for CK and SMA.None of the patients had present malignant transformation or metastasis.Conclusions GCT can occur in any part of the body,but is not common in breast.GCT is similar to breast cancer in clinical manifestations,imaging and macroscopic observation,etc.The correct diagnosis of this lesion depends on HE morphology,immunohistochemistry and special dyeing.The close postoperative follow-up should be performed.

Clinical features of three pulmonary alveolar proteinosis patients / 临床儿科杂志

Objective To investigate the diagnosis and treatment of pulmonary alveolar proteinosis (PAP). Methods Clinical data of 3 children with PAP admitted from 2010 to 2016 were retrospectively analyzed. Results Three children were female and aged 3 years and 1 month, 3 years and 9 months, and 6 years and 4 months, respectively. The main symptom of the three children was cough along with or without anhelation. Two of them had longer course of disease. The therapeutic effect of conventional treatment was poor, and their chest CT indicated significant lesions. Fiberoptic bronchoscopy showed positive reaction by PAS staining, and the treatment effect was improved after bronchoalveolar lavage. Conclusions The main clinical symptoms of PAP are non-specific cough and shortness of breath. PAS staining and bronchoalveolar lavage are of great value in the diagnosis and treatment of PAP.

A comparative study on the effect of fixation for guinea pigs eyeballs among three different fixation solution / 国际眼科杂志(Guoji Yanke Zazhi)

· AIM:To compare the effect of fixation and select the optimal fixation solution and condition for PAS staining of guinea pig eyes among three different fixation solution:4％ paraformaldehyde solution,4％ glutaraldehyde solution and Davidson solution.· METHODS:Totally 30 healthy guinea pigs were divided into 6 groups:Group Ⅰ-Ⅱ were fixed in 4％ paraformaldehyde solution and 4％ glutaraldehyde solution for 24h,respectively;Group Ⅲ-Ⅴ were fixed in Davidson solution for 3,6 and 24h,respectively;and Group Ⅵ were fixed in Davidson solution for 3h and then transferred into 10％ neutral formaldehyde solution for 48h.All groups were sectioned by routine section method and undergone PAS staining,and then observed by light microscope.· RESULTS:It was found that the group which was fixed by Davidson solution for 3h,remained the most complete structure for PAS staining (Group Ⅲ).While the effect of fixation for the group which was transferred into 10％ neutral formaldehyde solution for preserving for 48h after fixing in Davidson solution for 3h was also acceptable for PAS staining (Group Ⅵ).The retinal cells remained clear and in order for both groups which was mentioned above.· CONCLUSION:The best fixation condition for PAS staining for eyes of guinea pigs is fixation in Davidson solution for 3h among these fixation conditions,while it is also suitable to transfer the eyes into neutral formaldehyde solution after fixing in Davidson solution for 3h for preserving for long periods,which is not severely reduced the effect of fixation for PAS staining.

Clinical effect of 23 - gauge pas plana vitectomy for delay-onset endophthalmitis following cataract surgery / 国际眼科杂志(Guoji Yanke Zazhi)

AIM: To discuss the clinical effect of 23-gauge pas plana vitrectomy ( PPV) for delay-onset endophthalmitis following cataract surgery.? METHODS: This retrospective chart review study included patients with delay-onset endophthalmitis that was present 6wk or more after cataract surgery, who underwent 23-gauge PPV from January 2010 to January 2016 at Renmin Hospital of Wuhan University. The diagnosis was made according to clinical symptoms and signs. The postoperative follow - up results were recorded, including anterior segment examinations, fundus examinations, and best-corrected visual acuity.?RESULTS:Totally 15 patients (15 eyes) were included. The average time between cataract surgery and their eye symptoms of endophthalmitis was 5. 13 ± 2. 41mo. The culture isolates revealed that 9 eyes ( 60％) were cultured positive, anaerobes in 4 eyes (44％), fungal infections in 3 eyes ( 33％) , aerobes in 2 eyes ( 22％) . The mean follow-up period was 12mo. The final visual outcomes were as follows:11 eyes ( 73％) achieved a better vision with PPV; 3 eyes ( 20％) achieved a better vision with PPV, total capsulectomy ( TC ) and IOL removal; 1 eye ( 7％) with PPV, silicone oil tamponade, total capsulectomy ( TC ) and IOL removal was enucleated because of uncontrolled inflammation.?CONCLUSION: The 23-gauge PPV is safe and effective for the management of delay - onset endophthalmitis following cataract surgery. Recurrence rate might be decreased with total capsulectomy and IOL removal.

Avaliação dos aspectos da Personalidade em pessoas idosas na cidade de São Paulo por meio do Rorschach Performance System (R-PAS) / Evaluation of personality aspects in elderly people in Sao Paulo city using Rorschach Performance System (R-PAS) / Evaluación de los aspectos de la personalidad en las personas mayores en la ciudad de São Paulo a través del Rorschach Performance System (R-PAS)

A proposta deste estudo é identificar aspectos da personalidade que predominam em uma amostra de idosos. Foram avaliadas cinquenta pessoas com idades entre 60 e 85 anos residentes na cidade de São Paulo e sem história psiquiátrica. Comparado à amostra normativa internacional de adultos, foi possível verificar uma visão mais simplista e distorcida da realidade, embora sem confusão de pensamento, além de dificuldade cognitiva e necessidade maior de estimulação diante das demandas do ambiente.

The purpose of this study is to identify aspects of personality that predominate in a population sample of elderly individuals. Fifty people aged between 60 and 85 years old with no psychiatric history were evaluated. In comparison with the international normative sample of adults, it was possible to verify a more simplistic and distorted vision of reality, however with no thought disturbance, and some cognitive difficulty, as well as a greater need of stimulation as a result of the environment demands.

La propuesta de este estudio es identificar aspectos de la personalidad que predominan en una muestra de ancianos. Se evaluaron cincuenta personas con edades entre 60 y 85 años residentes en la ciudad de São Paulo y sin historia psiquiátrica. En comparación con la muestra normativa internacional de adultos, fue posible verificar una visión más simplista y distorsionada de la realidad, aunque sin confusión de pensamiento, además de dificultad cognitiva y necesidad mayor de estimulación ante las demandas del ambiente.

La femme mal barrée. Croyances en La femme, de l‘hystérie aux théories psychanalytiques / A mulher mal barrada. Crenças na mulher, da histeria às teorias psicanalíticas / The not wholly barred Woman. Beliefs in Woman, from hysteria to psychoanalytic theories / La mujer mal barrada. Creencias en La mujer, de la histeria a las teorías psicoanalíticas / Die schlecht gestrichene Frau. Ansichten zur Frau, von der Hysterie zu den psychoanalytischen Theorien

La question “qu’est-ce qu’une femme?” et la logique de l’exception sur laquelle se règle l’hystérique dans son rapport à la féminité témoignent de sa croyance selon laquelle la barre portée par Lacan sur La femme n’existe pas. La distinction entre cette logique et la logique féminine du pas-tout sera l’occasion de retracer les croyances sur la féminité dans la théorie psychanalytique et d’interroger les usages paradoxaux qui sont faits des positions dites féminine et masculine.

A pergunta “O que é uma mulher?” e a lógica da exceção pela qual a histérica se rege em sua relação com a feminilidade dão testemunho de sua crença de que não existe a barra colocada por Lacan sobre a Mulher. A distinção entre essa lógica e a lógica feminina do “não todo” será ocasião para situar novamente as crenças sobre a feminilidade no marco da teoria psicanalítica e para interrogar os usos paradoxais das posições “feminina” e “masculina”.

The question “What is a woman?” and the logic of exception on which a hysterical woman bases herself in her relationship with femininity reveal one of her beliefs: that the bar placed by Lacan on Woman does not exist. The distinction between this logic and the feminine logic of the “not-whole” is the opportunity to once again place beliefs on femininity within psychoanalytic theory and to question the paradoxical uses of “feminine” and “masculine” positions.

La pregunta “¿Qué es una mujer?” y la lógica de la excepción, que rige a la histérica en su relación con la femineidad, atestiguan sobre su creencia de que no existe la barra colocada por Lacan sobre la mujer. La distinción entre esta lógica y la lógica femenina del “no todo” será la ocasión para volver a situar las creencias sobre la femineidad en la teoría psicoanalítica y para interrogar los usos paradójicos de las posiciones “femenina” y “masculina”.

Die Frage „Was ist eine Frau?“ und die Ausnahmelogik wonach sich die Hysterikerin in ihrem Bezug auf die Weiblichkeit richtet, zeugen von ihrem Glauben daran, dass der Strich, mit dem Lacan Die Frau gestrichen hat, nicht existiert. Die Unterscheidung zwischen dieser Logik und der weiblichen Logik des „nicht-alles“ ist Anlass dazu, die übernommenen Vorstellungen zur Weiblichkeit innerhalb der psychoanalytischen Theorie neu zu positionieren, sowie die paradoxe Benutzung „weiblichen“ und „männlichen“ Positionen zu hinterfragen.

Mesenchymal stem cell isolation,identification and protection mechanism in kidney of diabetic rats / 中国免疫学杂志

Objective:To observe the bone marrow mesenchymal stem cell isolation,identification methods and protection mechanisms in the kidney of diabetic rats.Methods: Thirty-one rats were used to isolate bone marrow mesenchymal stem cells (BMSCs) by adherent culture method.Cell morphology was observed by phase contrast microscope.The surface molecular weight of cultured cells was detected by Real-time PCR.30 rats were induced by intraperitoneal injection of streptozotocin to establish diabetic rat model (n=15).And stem cell treatment group (n=15) according to the different treatment methods.Rats in the control group were treated with insulin combined with probucol.Stem cell treatment group was implanted with stem cells.Before and after treatment,fasting blood glucose,24 h urinary protein excretion,creatinine clearance rate and kidney weight were measured.PAS staining.And the renal tissue was detected by Western blot.Results: The primary bone marrow mesenchymal stem cells (MSCs) were cultured for 24 hours,and the cells were irregularly distributed in the culture flask.After 7 days of culture,the cells were regular,elongated,elliptical,and strong refraction,and the cells were fused with each other.The morphology of BMSCs was uniform and showed a long fusiform shape,CD44,CD31 and CD34 were observed by RT-PCR.The levels of fasting blood glucose,24 h urine protein excretion and creatinine clearance rate in the observation group were significantly higher than those in the control group (P0.05).Conclusion: It is suggested that BMSCs can be isolated from rat bone marrow mesenchymal stem cells by using adherent culture method and labeled with green fluorescent protein in vitro.Bone marrow mesenchymal stem cells can protect the kidney and inhibit the oxidative stress of diabetic kidney.

Faux Pas Test in schizophrenic patients / Teste de Faux Pas em pacientes com esquizofrenia

ABSTRACT Objective The aim of this study is to validate the adult version of “Faux Pas Recognition Test” created by Stone and colleagues (1998) as a reliable instrument assess and discriminate social cognition among schizophrenia patients and healthy controls. Methods This is a cross-sectional study with a total of 196 participants (mean age = 26.45; CI (95%) [25.10; 27.83]) 51% male. From those, 44 (22.4%) patients with schizophrenia and 152 (77.6%) healthy controls. The participants answered a short version of the Faux Pas Recognition Test, composed by 10 stories. Results Significant differences were found between both groups regarding their scores on Faux Pas Recognition Test (p = 0.003). Patients with schizophrenia had lower score, compared to healthy controls. Story 14 was the best to distinguish both groups, and Story 16, the worst. Among the questions of Faux Pas stories, the one related to intuition presented the most significant difference between the groups (p = 0.001), followed by the one related to understanding (p = 0.003). Conclusion The Brazilian version of the Faux Pas Recognition Test is a valid test to assess social cognition in schizophrenia and can be an important instrument to be used on the clinical practice.

RESUMO Objetivo O objetivo deste estudo é validar a versão brasileira da versão adulta do Teste de Reconhecimento de Faux pas criado por Stone e colaboradores (1998) como um instrumento confiável para avaliar e discriminar a cognição social entre pessoas com esquizofrenia e controles saudáveis. Métodos Trata-se de um estudo transversal com um total de 196 participantes (idade média = 26,45; IC (95%) [25,10; 27,83]), sendo 51% homens; destes, 44 (22,4%) eram pessoas com esquizofrenia e 152 (77,6%), controles saudáveis. Os participantes responderam a uma versão reduzida do Teste de Reconhecimento de Faux Pas, contendo 10 histórias. Resultados Foram encontradas diferenças significativas entre grupos no Teste de Reconhecimento de Faux Pas (p = 0,003). Pessoas com esquizofrenia obtiveram menor pontuação comparadas com o grupo controle. A história 14 foi a mais eficiente para distinguir os grupos, ao passo que a história 16 foi a pior. Quanto às questões das histórias Faux Pas, aquela referente à intuição foi a que apresentou maior diferença entre grupos (p = 0,001) seguida por compreensão (p = 0,003). Conclusão A versão brasileira do Teste de Reconhecimento de Faux Pas é um instrumento válido para avaliar a cognição social em esquizofrenia e pode ser um instrumento importante a ser usado na prática clínica.

MODELO INTEGRADO DE GESTIÓN DE ACTIVOS HOSPITALARIOS BASADO EN LA PAS 55 / INTEGRATED MANAGEMENT MODEL OF HOSPITAL ASSETS BASED ON PAS 55 / MODELO INTEGRADO DE GESTÃO DE ATIVOS HOSPITALARES COM BASE NA PAS 55

Este trabajo presenta un modelo de gestión de activos hospitalarios basado en la PAS 55, que se desarrolló a partir de la homologación de los requerimientos legales, de acreditación nacional e internacional y las recomendaciones de la Organización Mundial de la Salud OMS, la Pan American Health Organization PAHO y el Ministerio de la Protección Social. Se busca con este proyecto involucrar el ciclo de vida de los activos hospitalarios, plantear herramientas para la toma de decisiones frente a la adquisición y reposición de activos hospitalarios, plantear de la mano con los criterios del sector salud metodologías de evaluación de criticidad de los activos físicos hospitalarios, y definir metodologías y herramientas que faciliten la adecuada gestión de las instituciones frente al cuidado de los activos físicos y los riesgos asociados a los mismos.

This paper presents a management model on hospital assets based on PAS 55, which evolved from the approval of the legal requirements, national and international accreditation, and the recommendations of the World Health Organization -WHO, the PAHO, Pan American Health Organization and the Ministry of Social Protection of Colombia. This project seeks to include lifecycle of hospital assets, introduce new tools for decision making to the acquisition and replacement of hospital assets, uniting the criteria of health methodologies sector in evaluation of critical hospital critical assets and defining methodologies and tools to facilitate the adequate management of institutions for physical assets care and associated risks.

Neste artigo se apresenta um modelo de gestão de ativos hospitalares baseado na PAS 55, que evoluiu a partir da aprovação dos requisitos legais, de acreditação nacional e internacional e as recomendações da Organização Mundial da Saúde OMS, a Pan American Health Organization PAHO e do Ministério da Proteção Social. Destina-se com este projeto involucrar o ciclo de vida de ativos hospitalários, planejar ferramentas para fazendo para a toma de decisões frente para a aquisição e substituição de ativos hospitalários, planejar da mão para os critérios de avaliação do sector saúde metodologias de avaliação nos ativos físicos hospitalários criticidade e definir metodologias e ferramentas que facilitem o adequada gestão das instituições frente o cuidado de ativos físicos e risos associados a eles.

Transcatheter arterial infusion chemotherapy increases expression level of miR‑142‑5p in stage III colorectal cancer.

OBJECTIVE: To investigate the expression level of miR-142-5p and its potential target gene endothelial PAS domain protein 1(EPAS1) in Stage III colorectal cancer during Transcatheter arterial infusion chemotherapy (TAI). MATERIALS AND METHODS: Illumina high-throughput sequencing was used to obtain miRNA expression profiles of paired tumor and adjacent normal tissues from one patient received TAI 1 week before the operation and another patient directly underwent an operation. The expression levels of miR-142-5p was measured with both high-throughput sequencing and quantitative real time-polymerase chain reaction. RESULTS: The expression levels of miR-142-5p, were significantly reduced in tumor tissues of stage III CRC, then significantly increased in tumor tissues receiving TAI and higher than tumor tissues without TAI. The apoptosis rate of HT-29 colon cancer cells was mildly increased after transfection with pre-miR-142. miR-142-5p could bind directly to the 3´untranslated region of endothelial PAS domain protein 1 and reduce its expression. CONCLUSIONS: miR-142-5p is a potential tumor suppressor in CRC and is upregulated in tumor tissues after TAI, suggesting its potential clinical values for testing the functionality of TAI and predicting the progress of CRC.

Evidências de validade do Rorschach Performance Assessment System no diagnóstico da esquizofrenia / Evidence of validity of Rorschach Performance Assessment System in the diagnosis of schizophrenia / La evidencia de la validez del Rorschach Performance Assessment System en el diagnóstico de la esquizofrenia

O Método de Rorschach tem-se mostrado um eficaz instrumento para avaliação da personalidade, bem como para identificação de padrões psicopatológicos, em especial, a esquizofrenia. O objetivo do presente estudo foi buscar evidências de validade para o Rorschach Performance Assessment System (R-PAS) para o contexto brasileiro. Foram administrados o R-PAS e a Magical Ideation Scale (MIS) em 70 setenta adultos, divididos em 35 pacientes com esquizofrenia e 35 não pacientes. Os resultados das comparações evidenciaram diferenças estatisticamente significativas para as variáveis (Hd), An, FQo, FQu, FQn, P, DV, DR, INC, CON, MAH, MAP, GHR, PHR, EII-3 e TP-Comp (p<0,05). Foram encontradas associações positivas e de forte magnitude para o escore obtido na MIS e para as variáveis do R-PAS, EII-3 e TP-Comp (r=0,84 e 0,85, respectivamente). Assim sendo, interpretam-se os resultados como evidências de validade para o R-PAS no contexto brasileiro...

The Rorschach method has proven an effective tool for personality assessment and identification of psychopathological patterns, in particular schizophrenia. The aim of this study was to seek validity evidences for the Rorschach Performance Assessment System (R-PAS) in a Brazilian context. Both R-PAS and the Magical Ideation Scale (MIS) were administered in 70 adults, 35 schizophrenic patients, and 35 non-patients. The results of the comparisons showed statistically significant differences for the variables (Hd), An, FQo, FQu, FQn, P, DV, DR, INC, CON, MAH, MAP, GHR, PHR, EII-3 and TP-Comp (p<0.05). There were positive and strong magnitude associations to the score obtained in MIS and the variables of the R-PAS, EII-3 and TP-Comp (r=0.84 and 0.85, respectively). Therefore the results can be interpreted as evidence of validity for the R-PAS in a Brazilian context...

El método de Rorschach ha demostrado ser una herramienta eficaz para la evaluación de la personalidad y la identificación de patrones psicopatológicos, en particular la esquizofrenia. El objetivo de este estudio fue buscar evidencia de validez para el Rorschach Performance Assessment System (R-PAS) en contexto brasileño. Se administraron el R-PAS y la Magical Ideation Scale (MIS) en 70 adultos divididos en 35 pacientes con esquizofrenia, y 35 no pacientes. Los resultados de las comparaciones mostraron diferencias estadísticamente significativas para las variables (Hd), An, FQo, FQu, FQn, P, DV, DR, INC, CON, MAH, MAP, GHR, PHR, EII-3 y TP-Comp (p<0,05). Se encontraron associaciones positivas y magnitud fuerte a la puntuación obtenida en las MIS y las variables del R-PAS, EII-3 y TP-Comp (r=0,84 y 0,85, respectivamente). Así, se interpretan los resultados como evidencia de validez para el R-PAS en el contexto brasileño...