RESUMEN
【Objective】 To investigate the influence of different packaging methods on the volume of low-dose(0.5 U) suspended leucocyte depleted red blood cells(SLD RBC) and provide reference for accurate labeling. 【Methods】 Bags of SLD RBC in 1.5 U and 2 U were randomly sampled to measure the weight and specific gravity of each bag, so as to estimate the blood volume.The relationship between the weight and volume of 0.5 U blood, split from different parent bags, was analyzed and the linear regression equation was put forward.The regression equation was used to calculate and analyze the difference in the volume of 0.5 U SLD RBC prepared by three different packaging methods (A: manual multi-bag average packing; B: instrument multi-bag average packing; C: manual single-bag packing) in actual work. 【Results】 The specific gravity of 1.5 U (38 bags) and 2 U SLD RBC (39 bags) were (1.090±0.011) g/mL and (1.097±0.013) g/mL, respectively, and the difference was statistically significant (P<0.05). After the 0.5 U subsidiary bags were split from the parent bags(1.5 U or 2 U), the regression equations for the volume (Y) of 0.5 U and gross weight (X) of the whole bag were respectively: Y1.5 U packing=0.902 7X-12.52 (P<0.05) and Y2 U packing=0.905 6X-13.15(P<0.05). In actual blood packaging, the average blood volume of 0.5 U subsidiary bags split from 1.5 U bag, using method A and B, were smaller than those split from 2 U bag [(62.12±5.38) mL and (62.50±6.77) mL vs (67.72±3.81) mL and(68.39±6.44)mL] (P<0.05), with the deviation of low-dose blood volume from 5.593 mL to 5.887 mL.The volume deviation by method B (10.84% and 9.42%) were greater than that by method A (8.67% and 5.63%). The average volume of 0.5 U subsidiary bags split from 1.5 U and 2 U by method C were (65.49±1.72) mL and (64.99±1.91) mL (P>0.05), with volume deviation at 2.63% and 2.94%, respectively.The mean volume value of overall 0.5 U blood (n=483) was (65.35±5.34) mL. 【Conclusion】 For packaging 0.5 U subsidiary bags, the instrument multi-bag packaging showed the largest volume deviation, followed by the manual multi-bag packaging and the single-bag packaging.The volume labeling of low-dose SLD RBC should be established, according to the specifications of parent bags and specific gravity.
RESUMEN
Objective: To explore the suitable packaging methods for Rhei Radix et Rhizoma (RRR) with the contents of active ingredients, quality loss before and after storage, shelf life, and economic benefit as indexes. Methods: Rheum palmatum was used in the experiment and the color of RRR was studied according to Chinese Pharmacopeia 2010. The extract was obtained by hot-dip method, and the contents of anthraquinone, gallic acid, and catechin were determined using HPLC. Results: The moisture capacity of RRR package had the significant effect on the active constituents, the highest content of extracting substances (31.36%) was obtained with the moisture content of 24%, and the lowest content was obtained with the moisture content of 14%. The highest anthraquinone content (1.893%) was obtained with the moisture content of 14%, and the highest in catechin and gallic acid contents were 1.492% and 0.400% with the moisture content of 20%. The contents of RRR extract and anthraquinone were the highest (33.47% and 1.891%) with vacuum packing, while those with common package were the lowest (28.30% and 1.539%). The highest contents of gallic acid and catechin (0.323% and 2.022%) were obtained with deoxidizer packaging. The mildew and infestation observation and the quality determination of one-year stored RRR showed that the vacuum packing had no mildew with the moisture content of less than 20%. Conclusion: The vacuum packing is an optimal atmosphere packaging method, which could increase the revenue of farmers and the moisture content of RRR during storage.