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Objective To investigate the etiology,clinical manifestations,imaging finding,pathology and treatment of primary bladder schwannoma.Methods A case of bladder schwannoma was reported and discussed in the literature.A 64-year-old male patient with painless gross hematuria for 4 months was admitted on October 5,2016.Enhanced computed tomography (CT) showed left anterior bladder wall lesions with mildly enhancement.Preoperative diagnosis was bladder cancer.The patient underwent transurethral resection of bladder tumor (TURBT).During surgery,a 3 cm × 3 cm polypoid soft tissue was found in the left side of bladder,which convex to the bladder with smooth surface and wide base.Results The bladder neoplasm was resected successfully.The intraoperative bleeding was about 100 ml.Postoperative pathology showed a large number of myloid spindle cells with immunohistochemical S-100 (+),considering source of mesenchymal tissue.No recurrence was noticed during the 3 months' follow-up.Retrieving domestic and foreign literature,we found 17 cases with bladder schwannoma from 1993 to 2016.Bladder schwannoma occurs in the age of 40-69 years old.There is no relationship with the agenda.It is often seen in the top or the side wall of the bladder with single growth and rare malignant.The clinical manifestations was mainly painless gross hematuria,CT and magnetic resonance imaging(MR) showed less specificity than other solid tumors of the bladder,which is difficult to identify.Partial cystectomy or TURBT is the main strategy.Postoperative pathology is the gold standard for diagnosis.The immunohistochemical stainings often showed S-100(+).Conclusions Bladder schwannoma is extremely rare in benign bladder tumor,and it could easily be misdiagnosed.The diagnosis should be performed by histopathological examination.Because it will become malignant,it is suggested that the positive treatment should be done.Treatment methods have not been clearly defined,and the effect of surgical resection is good.
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Objective:To investigate the rapid establishment of nude mice-C6 glioma model and its application feasibility in research experiments.Methods:C6 mice glioma cell-suspension was inolulated to the male and female nude mice subcutaneously.The period for tumor tissue to grow out was detected and the histological and immunohistochemical features of tumor were observed.Tumor tissue was used to do allograft transplant for the male and female nude subcutaneously.The sprouted time of subcutaneous transplant tumor in nude mice was detected.Pathological and immunohistochemical features of tumor were studied.Results:(1)Success rates of both two methods were 100%,tumor grew in good condition,and the histological and immunohistochemical examination results of tumor were consistent with glioma cell morphology;(2)The method of cell inoculation to establish model took a long time.Tumor growing speeds in male and female nude mice were different and the difference had statistical significance(P0.05) Conclusion:(1)Tumor tissue transplant can establish subcutaneous xenograft model quickly,and to provide the reliable animal model for studying of glioma、biological characteristics and gene therapy;(2)Tumor tissue allograft can shorten the establishment time of animal models saving the cost of scientific research.