Experimental study on the effect of surmin on penile squamous carcinoma cell line(CUPE-1) / 대한비뇨기과학회지

Experimental study was done to investigate the effect of suramin on the in vitro and in vivo proliferation and metastasis of penile squamous carcinoma cell line(CUPE-1), morphological changes of CUPE-1 cells induced by suramin and mechanism of action of suramin. Suramin inhibited in vitro proliferation of CUPE-1 significantly with 1C50 of 100 microgram/ml media. In vitro antiproliferative effect of suramin on CUPE-1 was reversible after stopping administration of the drug. Weekly intraperitoneal administration of 200 mg/kg of suramin to nude mouse inhibited the proliferation and metastasis of intraperitoneally implanted CUPE-1 cells significantly. but did not show significant effect on the proliferation of subcutaneously implanted CUPE-1 cells. Suramin induced senile changes on ultrastructure of CUPE-1 cells. Suramin of 300 microgram/ml inhibited the prolireration-stimulating effect of EGF significantly, whereas, suramin of 100 microgram/ml did not inhibit the effect of EGF significantly. Suramin did not show significant cytotoxicity on 3H-thymidine release assay. These results suggest that suramin is a promising drug for the treatment of advanced penile squamous cell carcinoma and blood level of suramin in clinical trial should be continuously maintained in about 300 microgram/ml, and that the main machanism of suramin against CUPE-1 is cytostatic. by antagonizing the action of EGF and inducing growth arrest and senile change.

New penile squamous carcinoma cell line(CUPE-1): Characterization, tumor marker(TA-4) and growth factor study / 대한비뇨기과학회지

Chung-Ang University Penile Squamous Carcinoma cell line (CUPE-1) was established from a lymph node metastasis of human penile squamous cell carcinoma (SCC). CUPE-1 grew as adherent monolayer with a defined doubling time of 24 hours. CUPE-1 showed epithelial characterization on inverted and light microscopy and showed well developed desmosomes and tonofilaments or electron microscopy. CUPE preserved cytokeratin on immunohistochemical staining. CUPE expressed the receptor of epidermal growth factor (EGF), which stimulated the proliferation of CUPE-1 CUPE-1 showed strong tumorigenecity and/or metastatic ability when subcutaneously and intraperitoneally implanted into the nude mouse. CUPE-1 produced tumor associated antigen-4 (TA-4), a tumor marker for SCC of uterine cervix, both in vitro and in vivo. In addition, the serum level-of TA-4 were specifically increased in patients with penile SCC. These results indicate that CUPE-1 retains the characteristics of human penile SCC and could provide an excellent model for the basic research and development of new therapeutic modalities of penile cancer, and that TA-4 may become a valuable tumor marker of penile SCC.