Research progress on periodontal regeneration of replanted teeth after complete avulsion / 口腔疾病防治

@#Complete avulsion of teeth are one of the most serious traumatic dental injuries. The preferred treatment is tooth replantation. When teeth usually cannot be treated in time, the periodontal membrane on the root surface becomes necrotic , and the success rate of replantation is greatly reduced. How to use seed cells, growth factors and scaffold materials to promote periodontal tissue regeneration, improve periodontal membrane healing of replanted teeth, and achieve successful replantation of completely dislocated teeth is an urgent problem. Periodontal ligament stem cells (PDLSCs) express stem cell markers, have the potential to undergo multicellular differentiation and have important application value in periodontal tissue regeneration and repair. Fibroblast growth factor-2 (FGF-2), basic fibroblast growth factor (bFGF), lysobisphosphatidic acids (LPA) and other related regulatory factors promote periodontal tissue regeneration. Seed cells combined with scaffold materials can promote periodontal tissue regeneration and repair periodontal tissue defects. At present, studies have shown that PDLSCs and other seed cells have the potential for periodontal tissue regeneration and have been widely studied in experimental animal models of periodontitis and bone defects. However, there are few reports on the application of replanted teeth after complete avulsion, and its mechanism is not clear. In this paper, the research progress of periodontal tissue regeneration after total dislocation replantation is reviewed. We expect to apply periodontal tissue regeneration research to the replantation of fully dislocated teeth to achieve periodontal membrane healing of fully dislocated replanted teeth to provide a theoretical basis for future clinical work.

SEM Evaluation of the effect of root conditioning with citric acid: An In-Vitro study

Background Root conditioning agents reveal the orifices of dentinal tubules and the dentin collagen matrix and provide a substrate which plays a role chemotaxis migration and proliferation of cells involved in periodontal healing and formation of new connective tissue attachment. Materials and methods A total of 15 extracted teeth which were periodontally involved were utilised for the study. All the teeth received 25% citric acid application. The samples were subjected to scanning electron microscope evaluation. The photomicrographs were analysed by image software analyser to assess its surface area. Results Group II showed better results for mean surface area. Conclusion: These results show that citric acid could be a better healing biomaterial in periodontal regeneration by increasing surface area.

Use of Fusidic Acid for Desquamative Gingivitis Treatment: 1-Year Follow-Up

This study evaluated retrospectively the effect of fusidic acid on the management of desquamative gingivitis (DG). The study population consisted of 15 patients with DG. Patients were requested to make topical application of 2% fusidic acid ointment 4 times a day for 6 weeks. Clinical assessments were recorded at baseline, at 6 weeks and 12 months after beginning the therapy. Patients' examination involved lesion size area, pain score and impact on daily activities. All patients presented lesions in maxilla; in 9 patients (60.0%) lesions were predominately at the anterior region and 6 (40.0%) at the posterior region of maxilla. Treatment significantly (p<0.05) reduced the pain intensity (from 5.4±1.12 to 1.16±0.97) and its periodicity (from 53.33% with pain>3 x/week to 13.33%), and the lesion size in 72.47% (±4.12) immediately after 6 weeks of treatment. Improvements were sustained for 12 months compared to baseline (p<0.001). It also reduced the impact of disease in daily activities (eating and oral hygiene performance), and improved the emotional condition of patients, who reported better social relationships and habits. Topical application of fusidic acid may be a possible alternative local palliative therapy for desquamative gingivitis treatment.

Este estudo avaliou retrospectivamente o efeito do ácido fusídico sobre o manejo da gengivite descamativa (DG). A população do estudo consistiu de 15 pacientes com DG. Os pacientes foram solicitados a fazer aplicação tópica de pomada de ácido fusídico 2% 4 vezes ao dia, durante 6 semanas. As avaliações clínicas foram registradas no início do estudo, em 6 semanas e 12 meses após o início da terapia. O exame dos pacientes envolveu tamanho da área da lesão, intensidade da dor e impacto nas atividades diárias. Todos os pacientes apresentaram lesões na maxila; em 9 pacientes (60,0%) as lesões foram predominantemente na região anterior e em 6 (40,0%) na região posterior da maxila. O tratamento significantemente (p<0,05) reduziu a intensidade da dor (de 5,4±1,12 para 1,16±0,97) e a sua periodicidade (de 53,33% com dor>3x/semana para 13,33%), e o tamanho da lesão em 72,47% (±4,12) imediatamente após 6 semanas de tratamento. As melhorias foram sustentadas por 12 meses, quando comparado aos valores iniciais (p<0,001). Também reduziu o impacto da doença nas atividades diárias (alimentação e desempenho de higiene oral), e melhorou a condição emocional dos pacientes, que relataram melhores relações e hábitos sociais. A aplicação tópica de ácido fusídico pode ser uma alternativa para terapia local paliativa no tratamento de gengivite descamativa.

Autotransplantation of immature third molars; Pulp healing, periodontal healing and root development

PURPOSES: The aim of this study was to evaluate pulp healing, periodontal healing, root development of autotransplantation of immature third molars and show its viability in treatment of early loss of tooth in young patients MATERIALS AND METHODS: In this article we performed a retrospective study with 41 transplanted teeth in 36 patients. The Mean age at the time of surgery was 17 years(range 13-24 years) and mean postoperative follow up period was 2.4 years(range 1-6 years) We evaluated the survival rate, pulp healing, periodontal healing, root development of the above teeth RESULTS: At the last examination 40 teeth among 41 transplants were still present so survival rate was 97.6%. The pulpal healing was found in 38 teeth of 41 transplants. The periodontal healing was found in 38 transplants. The continuous root development was seen in 38 transplants. CONCLUSIONS: From the above results, the autotransplantation of immature third molars was found to be a useful and reliable treatment method for early loss of teeth in adolescents and young adults.

Conocimiento actual sobre el virus papiloma humano (vph) y su relación en la cavidad bucal y en el periodonto

The purpose of this revision is to describe the actual knowledge about Human Papiloma Virus (HVP) and its relation with the oral cavity and periodontium, supported by a bibliographic revision. A descriptive and documental investigation was conducted mainly by national and international literature (journals, books) and interviews with specialists. The HPV has a frequency between 36% and 74% among the population, and every day a huge number on any existing group is been diagnosed, is been described around 80 types. The HPV lesion related with bucal cavity and the periodontum are: papilloma, condyloma, focal epithelial hyperplasia, carcinoma verrugoso, leukoplakia, lichen planus, also has close relationship with HIV positive patiens. Among the periodontal treatments applied on this lesions are: Gingivectomy. Also, on this study a clinic case is reported, a nine years old male patient, assisted to the Periodontics Postgrade at the University of Zulia, School of Dentistry, having a previous history the presence of a oral lesion related with the HPV. Conclusions: so having this point on mind is important to identify these conditions to determine the right treatment plan and establish the sequence of the treatment chosen to improve the oral and periodontal health of the patient. Is important that the general dentist, the periodontist and the other specialties identify this pathologies and the consequences that might bring along if prevention is not done time, and always having the biosecurity norms present at the treatment time.

El propósito de esta revisión es describir los conocimientos actuales sobre el Virus Papiloma Humano (VPH) y su relación en la cavidad bucal y en el periodonto, soportado en una revisión bibliografica. Para ello se realizó una investigación descriptiva y documental principalmente con literatura nacional e internacional (revistas, libros) y entrevistas a especialistas en la materia. El VPH tiene una frecuencia entre 36% y el 74% de la población, y cada día se diagnostica un gran número en cualquier grupo etario, se describen alrededor de 80 tipos. Las lesiones del VPH relacionadas con la cavidad bucal y en el periodonto son: papiloma, condiloma acuminado, hiperplasia epitelial focal, carcinoma verrugoso, leucoplasia, liquen plano, además, tiene una estrecha relación con pacientes VIH positivos. Entre los tratamientos periodontales aplicados en estas lesiones se citan: la gingivectomía quirúrgica, electroquirúrgica, láser y quimioquirúrgica, siendo la mas utilizada la gingivectomía quirúrgica. Además, en el estudio se reporta un caso clínico de un paciente masculino, De 9 años de edad que asistió al área clínica del Postgrado de Periodoncia en la Facultad de Odontología de la Universidad del Zulia, teniendo como antecedentes la presencia de una lesión en boca relacionada con el Virus Papiloma Humano. De allí la importancia de identificar estas condiciones para determinar un correcto plan de tratamiento y establecer la secuencia del tratamiento elegido para mejorar la salud bucal y periodontal del paciente. Conclusión: Es importante que el Odontólogo General, el Periodoncista y los demás especialistas identifiquen estas patologías y las consecuencias que ocasiona si no se previene a tiempo, poniendo siempre en práctica las normas de bioseguridad al momento de trabajar.

Effect of alendronate on the healing of replanted dog teeth after extended dry time / 实用口腔医学杂志

Objective:To investigate the effect of alendronate(ALN) solution on the healing of replanted dog teeth after extended dry time. Methods:The first, second incisor and first premolar in maxilla and the second, third incisor and first premolar in mandible in two mature mongrel dogs were used for the test. The right tested teeth were included in experimental group, and the left in control. Firstly, all tested teeth were endodontically treated. Seven days later, they were extracted, then naturally dried for 60 min. The teeth in experimental group were soaked for 5 min in a 1 mmol/L solution of ALN before replantation. The teeth in control group were not treated with any other methods before replatation. Bone masses including teeth were taken out 3 monthes after replantation. The samples were examined histologically.Results:The average percentage of cementum healing in experimental group was higher than that in control group(P

An experimental study of tooth root replantation combined with in vitro cultured periodontal ligament cells / 实用口腔医学杂志

Objective:To test the periodontal regeneration potential after tooth root replantation combined with in vitro cultured periodontal ligament cells (PDLCs).Methods:The third and fourth mandibular premolars of two mongrel dogs were extracted and the dog PDLCs were in vitro cultured.The autologous roots combined with PDLCs were replanted into the created cavities in the right mandibular edentulous area one month after premolar extraction.Roots replanted without PDLCs on the left mandible served as the controls. The dogs were sacrificed 2 months after the replantation. Paraffin sections were made and stained with hematoxylin & eosin, then examined histologically.Results:In the samples of root combined with PDLCs implantation, some fiber bundles vertical to the surface of bone were embedded in some inner walls of the alveolar socket opposite to the apical root.Conclusion:Cultured PDLCs in vitro retain the capacity of PDL-like tissue formation even in the artificial alveolar socket.

Effect of local application of alendronate combined with tetracycline on the healing of replanted dog teeth after extended dry time / 实用口腔医学杂志

0.05).The inflammatory resorption of root in experimental group was lower than that in the control(P