Distribution and proportion of M1/M2 macrophages in periodontal tissues in rats with and without periodontitis / 口腔疾病防治

Objective@#To investigate the distribution and proportion of M1/M2 macrophages in the periodontal tissues of rats with and without periodontitis.@*Method@#Twelve Sprague-Dawley rats were randomly divided into a chronic periodontitis group (CP, n = 6) and a periodontal health group (PH, n = 6). The periodontitis model was induced at the first mandibular molar using a stainless steel ligature and was confirmed by histological analysis. M1 macrophages were labeled with inducible nitric oxide synthase (iNOS), and M2 macrophages were labeled with CD163. The distributions of M1 and M2 macrophages in the two groups were determined via immunohistochemistry and immunofluorescence, and the M1/M2 ratios were compared between the two groups.@*Results @#The M1 type macrophage count in the PH group was 12.17 ± 1.40, and the M1 macrophage count in the CP group was 40.00 ± 3.20; there was a statistically significant difference between the two groups (t = 7.96, P＜0.0001). The M2 macrophage count in the PH group was 4.50 ± 1.09, and the M2 type macrophage count in the CP group was 5.33 ± 0.67. There was no statistically significant difference between the two groups (t = 0.65, P = 0.53). The M1/M2 ratio in the CP group was 3.72 ± 1.08, and the M1/M2 ratio in the PH group was 8.31 ± 1.37; there was a statistically significant difference between the two groups (t = 2.63, P= 0.025).@*Conclusion@#During periodontitis, M1 macrophages increased significantly and were widely distributed; they may be involved in the progression of periodontitis and may be closely related to the destruction of the cementum.

Expression and distribution of TNF-α and PGE2 of periodontal tissues in rat periodontitis model

OBJECTIVE@#To simulate the expression of TNF-α and PGE2 of periodontal tissues in rat periodontitis model.@*METHODS@#40 Wistar rats were randomly divided into the periodontitis group and the control group (n=20). After the successful establishment of periodontitis rat model, raising for six weeks before the animals were sacrificed. The periodontal tissues were obtained and made into slices. Observed the histopathological changes of the periodontal tissues and measured TNF-α, PGE2 levels change by immunohistochemistry, Western blot analysis and ELISA.@*RESULTS@#TNF-α, PGE2 expression of the periodontitis group was significantly higher than that in the control group, the difference was significant (P<0.05).@*CONCLUSIONS@#The TNF-α, PGE2 expression of the rat periodontal tissue in the periodontitis group was significantly higher than the control group.

Effects of ginsenoside Rg-1 on the expressions of interleukin-6,bone gla protein in periodontal tissues in periodontitis rats / 实用口腔医学杂志

Objective:To determine the effects of ginsenoside Rg1 on the expression levels of bone gla protein(BGP) and cytokine interleukin-6(IL-6) in the periodontium of experimental periodontitis rats.Methods:One hundred 160-200 g Wistar rats were randomly divied into ten groups.Group A used as control were killed at the beginning of the experiment.Group B-J were experimental periodontitis models.Group B-E received the therapy of ginsenoside Rg1 were killed at 1st,2nd,3rd and 4th weeks.Group F-J received no treatment as periodontitis control were killed at 0,1st,2nd,3rd and 4th weeks.The expression levels of BGP and IL-6 in the periodontium were measured by immunohistochemistry.Results:The expression levels of BGP in the group F were significantly lower than that in the group A,the expression levels of IL-6 in the group F were much higher than that in the group A(P