RESUMEN
Background: Today Herbal medicine are commonly used because of their easy availability and affordability. It is more closely corresponds to the patient’s belief, concerns about the adverse effects of chemical (synthetic) medicines, persuade a desire for more personalized health care, and allows significant public access to health information. The major use of herbal medicines is for health promotion and treatment for chronic, as opposed to life-threatening, conditions. Usage of traditional remedies increases when conventional medicine is less effective in the treatment of disease, such as in advanced cancer and in the face of new infectious diseases. The hibiscus Rosa sinensis plant extract have multiple organic component like flavonoids, tannins, alkaloids, triterpenoids which are known to have antibacterial activity against E. coli, P aeruginosa, Salmonella species. Phytotherapy is considered to be less toxic and minimal or no side effects in comparison to modern allopathic medicines. Therefore, in today’s scenario there has been reappearance of interest developed in herbal medicine. Therefore, the study was planned to look for antibacterial activity of extract of petals and leaves of hibiscus Rosa sinensis against.Methods: Escherichia coli isolated from different clinical samples along with resistant antibiotics. All resistant isolates were tested along with hibiscus Rosa sinensis extract both leaves and petals ex-tract with resistant antibiotics.Results: We found that out of total 51, 35 (68.63%) were resistant E. coli isolates to different antibiotics and 85.72% were from urine samples. Enhancement effect in zone size with many resistant antibiotics was seen individually with both extracts. Antibiotics like ampicillin, cefotaxime, cotrimaxazole, cefuroxime, piperacillin, gentamycin showed more enhancement with rose petal extract than leaves. While antibiotic like levofloxacin (5%) showed enhancement only with leaves extract and not with the petals extract.Conclusions: It was found that Rose petal extract showed more antibacterial activity along with the antibiotics as compare to Rose leaves extract.
RESUMEN
In order to assess inhibitory potentials of white rose petal extracts (WRPE) on the activities of enzymes related to dermal aging according to the extraction conditions, three extraction methods were adopted. WRPE was prepared by extracting dried white rose (Rosa hybrida) petals with 50% ethanol (WRPE-EtOH), Pectinex(R) SMASH XXL enzyme (WRPE-enzyme) or high temperature-high pressure (WRPE-HTHP). In the inhibition of matrix metalloproteinase-1, although the enzyme activity was fully inhibited by all 3 extracts at 100 microg/mL in 60 min, partial inhibition (50-70%) was achieved only by WRPE-EtOH and WRPE-enzyme at 50 microg/mL. High concentrations (> or =250 microg/mL) of all 3 extracts markedly inhibited the elastase activity. However, at low concentrations (15.6-125 microg/mL), only WRPE-EtOH inhibited the enzyme activity. Notably, WRPE-EtOH was superior to WRPE-enzyme and WRPE-HTHP in the inhibition of tyrosinase. WRPE-EtOH significantly inhibited the enzyme activity from 31.2 microM, reaching 80% inhibition at 125 microM. In addition to its strong antioxidative activity, the ethanol extract of white rose petals was confirmed to be effective in inhibiting skin aging-related enzymes. Therefore, it is suggested that WRPE-EtOH could be a good candidate for the improvement of skin aging such as wrinkle formation and pigmentation.