RESUMEN
Objective To investigate the efficacy and safety of three injections fat soluble drugs to dissolve fat.Methods 3T3-L1 preadipocytes were treated with 5％ phosphatidyl choline (PC),4.5％ deoxycholate salts (DC) and lipostabil,respectively; thiazolyl tetrazolium (MTT) method was used to measure the different fat cell proliferation activity,the enzyme assay to measure liquid triglyceride (TG) content in culture media and to evaluate the degree of dissolution of the fat cells.16 Hartley white guinea pigs were randomly divided into four groups:shoulder (group a),scapular region (group b),hips (group c),and back (control group) were injected with fat soluble drug 0.5 ml in different parts of the guinea pig fat layer,and at different time points tissues were cut for pathological analysis.Results The proliferative activity of 3T3-L1 preadipocytes were significantly decreased after treatment with three types of fat soluble drugs compared with control group (P＜0.05),and their effects on dissolution of fat cells were also significant:the contents were 5％ PC (4.14±0.92)mmol/L,4.5％ DC (3.91 ±0.67) mmol/L,and lipostabil (4.23± 0.76) mmol/L,respectively,which were significantly higher than that of the control (1.91±0.12) mmol/L (P＜0.05); guinea pigs in vivo showed that the three types of fat soluble drugs on dissolving adipose tissue at the injection site had varying degrees of swelling,lymphocytic infiltration,and fat cell degeneration,fusion and decrease in number.Conclusions Three fat soluble injections could dissolve the fat cells in some degree,in whichi lipostabil is stronger than other fat soluble drugs,but their effect on adipose tissue is nonspecific,and therefore clinical application of those fat soluble drugs should be in high caution.
RESUMEN
Objective To investigate the effects of silybin-phosphatidylcholine compound (SPC) on H2O2-induced the production of nitric oxide (NO) and reactive oxygen species (ROS) in mouse macrophage.Methods Macrophage were collected in abdominal cavity of 6-8 week Kunming mouse,and cultured macrophage(2?108 L-1) were divided into control and SPC group randomly.Macrophage in control group were added into the same volume(0.1 mL) of 9 g/L sodium chloride.Macrophage in H2O2 group were added into a single bolus of H2O2 (1 mmol/L H2O2) for 30 min,while macrophage in SPC group were preincubated with different concentration of SPC for 2 h followed by a 30 min incubation with 1 mmol/L H2O2.Immunocytochemistry were used to measure the contents of inducible nitric-oxide synthase(iNOS),NO production was determined by Griess reactive, ROS was determined by DCFH-DA Fluorescence probe.Results The production of NO in H2O2 group markedly higher than that in control group(P