RESUMEN
Objective: To establish a method for the content determination of ginsenoside Rg1, Re, and Rb1 in Piwang Granules. Methods: HPLC-ELSD was used. The analysis was carried on Shim-pack VP-ODS (150 mm × 4.6 mm, 5 μm) column with mobile phase consisted of acetoneitrile-water, and the gradient elution (0-20 min, acetoneitrile 20%, 20-35 min, acetoneitrile 20%→30%, 35-55 min, acetoneitrile 30%, 55-56 min, acetoneitrile 30%→45%) was used. The flow rate was 0.8 mL/min. The column temperature was 25℃. The drift tube temperature was 100℃. The flow rate of gas was 3 L/min. Results: The responses of ginsenoside Rg1, Re, and Rb1 were liner in the ranges of 125.44-470.40 (r = 0.999 6), 88.96-333.60 (r = 0.999 2), and 157.12-589.20 μg/mL (r =0.999 5), respectively. The average recoveries were 100.27% (RSD = 1.713%, n = 9), 101.29% (RSD = 1.220%, n = 9), and 101.00% (RSD = 1.668%, n = 9). Conclusion: The method is simple, rapid, and with good repeatability. It can be used for the quality control of Piwang Granules.