Determination of myricetrin and quercitroside in Polygonum aviculare L / 国际中医中药杂志

Objective To establish the RP-HPLC method for the determination of myricetrin and quercitroside in Polygonum aviculare L.Methods The Agilent LC-C18 (250 mm×4.6 mm,5 μm) column was used,the mobile phase consisted of acetonitrile:0.1％ H3PO4(18 ∶ 82),the flow rate was 1.0 ml/min,the column temperature was 30℃ the detecting wavelength was at 256 nm.Results The calibration curve was linear within a range of 1.22～24.30 μg/ml and 0.77～15.40 μg/ml,the average recovery of this method was 98.9％,99.6％ and the RSD was 1.11％,1.09％,for the myricetrin and quercitroside respectively.Conclusion The method is simple,repeatable and accurate.It can be applied in quantitative determination of myricetrin and quercitroside in Polygonum aviculare L..