RESUMEN
OBJECTIVE: To observe the protective effects and mechanisms of Yiqi Huoxue Jiedu Recipe traditional Chinese medicine on cerebral ischemia and reperfusion injury. METHODS: SD rats were r andomly divided into sham-operation group, model group, saponin group, flavonoids group, polysaccharide group, combination group of saponin, flavonoids and polysaccharide, and nimodipine group. Using focal middle cerebral artery embolization(MCAO) model, the symptoms of neurological deficit score were observed;the infarct volume, brain index, brain water content, SOD activity, the contents of MDA, IL-1β, IL-6 and TNF-α of ischemic brain tissue, the expressions of Bcl-2 and Bax proteins, and the quantity of nissl Body were determined. RESULTS: The combination with saponin, flavonoids and polysaccharide, the main active part of Yiqi Huoxue Jiedu Recipe, could significantly reduce the symptoms of neurological deficits, promote the recovery of neurological deficits, narrow infarct volume of brain tissue, reduce the percentage of infarct volume, brain index and brain water, raise SOD activity, reduce the contents of MDA, IL-1β, IL-6 and TNF-α of ischemic brain tissue. It also increased Bcl-2protein, reduced Bax expression and reperfusion significantly. The reduction or disappearance of nissl body was inhibited. CONCLUSION: The combination with saponin, flavonoids and polysaccharide has significant protective effects on ischemic brain injury.