RESUMEN
AIM To investigate the therapeutic effect of ethyl acetate extract from Pongamia pinnata roots (PREA) on ethanol-induced gastric lesions. METHODS The experimental gastric mucosal injuries were prepared by ig ethanol to rats, and the protective effect of PREA was evaluated by calculating lesion index, observing pathological changes, and measuring the contents of nitric oxide (NO) and malondialdehyde (MDA), and the activity of superoxide dismutase (SOD) from gastric mucosal tissue. In addition, gastric secretary and gastric wall adherent mucus were studied with the pylorus-ligation rat model. RESULTSCompared with the model control group, PREA (50, 150 and 450 mg·kg-1, ig) dose-dependently prevented the gastric mucosal damages induced by ethanol, its inhibition rates were 28.7%, 57.7% and 78.7 %, respectively. The pathomorphology lesions of mucosal tissue were obviously ameliorated. PREA obviously antagonized the ethanol-induced elevation of MDA content, and reduction of NO level and SOD activity of gastric mucosa. PREA significantly reduced gastric juice volume, free acidity, total acidity and total acid output, but didn′t affect the pepsin activity. Moreover, PREA obviously increased adherent mucus quantity of stomach wall, as well as free mucus quantity dissolved in gastric juice of pylorus-ligation rat. CONCLUSIONPREA has protective effect on ethanol-induced gastric mucosal injuries, which suggests that PREA may be used for protection or treatment of human ethanolinduced gastric lesions.
RESUMEN
AIM: To study the effect of total flavonoids of Pongamia pinnata roots(PRF) on ulcerative colitis(UC) induced by dextran sulphate sodium(DSS) in mice and the possible mechanism. METHODS: Balb/c mice were fed with 4% DSS solution for 7 d to induce ulcerative colitis and treated with sulfasalazine(SASP) as positive control.The clinical symptom and the lesion of colonic mucosa were observed and the levels of T-AOC、MDA、NO and the activity of NOS in colonic tissue were tested. RESULTS: Compared with the model control,the clinical symptom and the lesion of colonic mucosa in treatment group were remarkably improved after administration of PRF in large or medium dose.In the two groups,the decrease in the levels of MDA,NO and the activity of NOS,and the increase in the levels of F-AOC could be found.(P0.05). CONCLUSION: The results indicate that significant effect of PRF on DSS-induced UC may attributae to reduction in free radical,depression of lipid peroxidation and enhancement of antioxidant capability.