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1.
Braz. j. microbiol ; 49(4): 785-789, Oct.-Dec. 2018. tab, graf
Artículo en Inglés | LILACS | ID: biblio-974292

RESUMEN

ABSTRACT Bovine viral diarrhea virus can cause acute disease in livestock, leading to economic losses. We show that Prostaglandin A1 inhibits bovine viral diarrhea virus replication in Madin-Darby bovine kidney cells (94% inhibition using 5 µg/mL). Light and electron microscopy of infected cells shows that Prostaglandin A1 also prevents virus-induced vacuolization, but at higher concentrations (10 µg/mL).


Asunto(s)
Animales , Bovinos , Antivirales/farmacología , Prostaglandinas A/farmacología , Diarrea Mucosa Bovina Viral/virología , Virus de la Diarrea Viral Bovina/efectos de los fármacos , Antivirales/análisis , Prostaglandinas A/análisis , Replicación Viral/efectos de los fármacos , Diarrea Mucosa Bovina Viral/tratamiento farmacológico , Línea Celular , Virus de la Diarrea Viral Bovina/fisiología , Virus de la Diarrea Viral Bovina/genética , Diarrea
2.
Rev. Soc. Bras. Med. Trop ; 51(5): 584-590, Sept.-Oct. 2018. tab, graf
Artículo en Inglés | LILACS | ID: biblio-957459

RESUMEN

Abstract INTRODUCTION: The Mayaro virus (MAYV), which is an arbovirus closely related to the Chikungunya virus, causes a dengue-like acute illness that is endemic to Central and South America. We investigated the anti-MAYV activity of prostaglandin A1 (PGA1), a hormone which exhibits antiviral activity against both ribonucleic acid (RNA) and deoxyribonucleic acid (DNA) viruses. Further, we examined the effects of inducting the stress protein HSP70 following PGA1 treatment. METHODS: Hep-2 cells infected with MAYV were treated with PGA1 (0.1-6μg/ml) 12h before infection and for different periods post-infection. Inhibition of viral replication inhibition was analyzed via viral titer determination, whereas the effect of PGA1 on viral morphogenesis was examined via transmission electron microscopy (TEM). Autoradiography (with 35S methionine labeling) and western blotting were used to assess the effect of PGA1 treatment on viral and cellular protein synthesis, and on HSP70 induction, respectively. RESULTS: PGA1 strongly reduced viral replication in Hep-2 cells, particularly when added during the early stages of viral replication. Although PGA1 treatment inhibited viral replication by 95% at 24 hours post-infection (hpi), viral structural protein synthesis was inhibited only by 15%. TEM analysis suggested that PGA1 inhibited replication before viral morphogenesis. Western blot and densitometry analyses showed that PGA1 treatment increased HSP70 protein levels, although this was not detectable via autoradiography. CONCLUSIONS: PGA1 inhibits MAYV replication in Hep-2 cells at early stages of viral replication, prior to production of viral structural proteins, possibly via HSP70 induction.


Asunto(s)
Humanos , Animales , Bovinos , Prostaglandinas A/farmacología , Replicación Viral/efectos de los fármacos , Alphavirus/efectos de los fármacos , Proteínas HSP70 de Choque Térmico/farmacología , Células Epiteliales/virología , Antivirales/farmacología , Línea Celular , Western Blotting , Alphavirus/ultraestructura , Microscopía Electrónica de Transmisión , Electroforesis en Gel de Poliacrilamida , Células Epiteliales/ultraestructura
3.
China Pharmacy ; (12)2007.
Artículo en Chino | WPRIM | ID: wpr-534435

RESUMEN

OBJECTIVE: To establish an HPLC method for the content determination of prostaglandin A1 and prostaglandin B1 in Alprostadil for injection.METHODS: The determination was performed on Alltech Alltima C18 column with mobile phase consisted of phosphate puffer(pH=6.3)-acetonitrile-methanol(70 ∶ 25 ∶ 5) at a flow rate of 1.5 mL? min-1.The detection wavelength was set at 196 nm.The column temperature was set at room temperature and the injection volume was 20 ?L.RESULTS: The prostaglandin A1 and prostaglandin B1 were well separated from main component and other impurities.The linear range of prostaglandin A1 and prostaglandin B1 were 0.175~19.00 ?g?mL-1(r=0.999 7) and 0.23~19.90 ?g?mL-1(r=0.999 2).The contents of prostaglandin A1 in 3 batches of samples were 4.7%,4.9% and 4.3%,and the contents of prostaglandin B1 in 3 batches of samples were 0.6%,0.8% and 0.5% respectively.CONCLUSIONS: This method is proved to be simple,specific and suitable for the content determination of related substances in Alprostadil for injection.

4.
The Korean Journal of Hepatology ; : 72-79, 2005.
Artículo en Inglés | WPRIM | ID: wpr-94679

RESUMEN

BACKGROUND/AIMS: Prostaglandin (PG) A2 has been reported to inhibit the growth of hepatocellular carcinoma cells via activation of apoptosis, although the molecular mechanisms involved have not been clarified, yet. To investigate the mechanism of the PGA2-induced apoptosis, we analyzed the activation of caspases during the apoptosis of hepatoma cell lines. METHODS: Induction of apoptosis by PGA2 in hepatoma cell lines, Hep 3B and Hep G2, was assessed by DAPI staining of nuclei and agarose gel electrophoresis of genomic DNA. The involvement of caspases was analyzed by immunoblot analysis of poly ADP-ribose polymerase (PARP) and by checking the effect of caspase inhibitors on PGA2-induced apoptosis. RESULTS: PGA2 inhibited the growth of Hep 3B and Hep G2 cells, accompanying nuclear condensation and fragmentation, and genomic DNA laddering, which are the hallmarks of apoptosis. The PARP was not cleaved during the apoptosis of Hep 3B and Hep G2 cells and caspase inhibitors such as z-VAD-Fmk and z-DEVD-Fmk exerted no effect on the PGA2-induced apoptosis. CONCLUSIONS: These results suggest that PGA2 induces apoptosis in Hep 3B and Hep G2 cells via caspase-independent pathway.


Asunto(s)
Humanos , Apoptosis/efectos de los fármacos , Carcinoma Hepatocelular/patología , Caspasas/antagonistas & inhibidores , Línea Celular Tumoral , Proliferación Celular/efectos de los fármacos , Activación Enzimática , Neoplasias Hepáticas/patología , Prostaglandinas A/farmacología , Células Tumorales Cultivadas
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