INHIBITION OF TETRANDRINE AND DEMETHYL TETRANDRINE ON DNA, RNA AND PROTEIN SYNTHESES OF LEUKEMIA 7712 AND SARCOMA 180 CELLS / 中国药理学通报

The 50% inhibition doses ( ID50 ) of tetrandrine and demethyl tetrandrine on DNA synthesis in L7712 and Sl80 cells are 2.6, 3 .5mg/ L and 27.5, 24.5mg/L respectively. The DNA synthesis inhibition is likely due to DNA template damage caused by both natural products. The inhibition of DNA, RNA and protein syntheses by tetrandrine or demethyl tetrandrine increased with incubating time. The inhibiting activities of two natural products on DNA and RNA synthesis are s-tronger than on protein. There are no differences either in inhibiting activity or in inhibiting mechanism between two natural products.