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Objective To determine the content of total flavonoids and luteolin from Pteris multifida Poir. Methods The content of total flavonoids was determined by gradient elution of macroporous resin D101 and ultraviolet spectrophotometry. The content of luteolin was determined by HPLC. The analysis was performed on a RP-C18 column(4.6 mm×250 mm, 5 μm) with aceconitrile-0.2% phosphoric acid (35:65) as the mobile phase at a flow rate of 1.0 ml/min, and 30 ℃ temperature. Results The detection of wave length was set at 349 nm. The content of luteolin was 0.015%, 0.019%, 0.016%, and the content of total flavonoids was 0.015%, 0.019%, 0.016%, respectively. Conclusions The method is suitable for the determination of flavonoids componets from Pteris multifida Poir.
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The materials were extracted by 95% ethanol, and the extracting solution was isolated by kinds of chromatographic columns including polyamide, MCI, preparative MPLC, and preparative HPLC. Eight diterpenes and two sesquiterpenes were isolated from the plant. On analysis of ESI-MS and NMR spectroscopic data, the structures were established as ent-3β-hydroxy-kaur-16-en-19-al (1), 4-epi-kaurenic acid (2), mitrekaurenone (3), 7β,16α,17-trihydroxy-ent-kauran-19-oic acid (4), crotonkinin E (5), crotonkinin F (6), pterisolic acid A (7), pterisolic acid C (8), (2R)-pterosin P (9), and dehydropterosin B (10). Compounds 1-6 were obtained from Pteris for the first time, and compounds 7-10 were obtained from P. ensiformis for the first time. Compounds 5-8 showed moderate activity against HCT-116, HepG2 and BGC-823 cell lines, separately.
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Aims: To isolate pure compounds from the methanolic fraction obtained from successive fractionation of defatted ethanolic extract and evaluate in vitro antioxidant and anticancer activity of the crude ethanolic extract, methanolic fraction and pure compounds isolated from methanolic fraction from leaves of Pteris multifida Poir. Study Design: Isolation and identification of the compounds, evaluation of antioxidant and anticancer activity on cervical cancer cell line (HeLa), lung carcinoma cell line (NCIH460) and breast carcinoma cell line (MCF-7). Place and Duration of Study: Vietnam Academy of Science and Technology of Ho Chi Minh City and School of Biotechnology, International University, Vietnam National University, Ho Chi Minh City, between December 2012 and September 2013. Methodology: The crude ethanolic leaf extract and methanolic fraction obtained from successive fractionation of defatted ethanolic extract from Pteris multifida leaves were prepared. The isolated compounds from methanolic fraction were identified using different spectroscopic techniques. Antioxidant activity of the samples was evaluated by using the stable free radical 2, 2- diphenyl picrylhydrazyl (DPPH). Sulforhodamine B (SRB) assay was exploited for determination of anticancer activity against three selected human cancer cell lines: HeLa, NCI-H460 and MCF-7. Results: Two main compounds were isolated from methanolic fraction obtained from successive fractionation of defatted ethanolic extract: rutin (1) and apigenin-7-O-β-Dglucopyranoside (2). The crude ethanolic leaf extract showed weak antioxidant activity (IC50 = 89.84 μg/mL) whereas the methanolic fraction expressed quite strong antioxidant activity (IC50 = 21.9 μg/mL). Rutin (1) showed a good ingredient of antioxidant activity with IC50 value of 37.70 ± 0.03 μg/mL. Crude ethanolic leaf extract had cytotoxic activity against HeLa and NCI-H460 cell lines while the methanolic fraction had cytotoxic activity against HeLa, NCI-H460 and MCF-7 cell lines. Apigenin-7-O-β-D-glucopyranoside (2) had strong anticancer activity against MCF-7 cell line with IC50 = 22.62 ± 0.59 μg/mL. Conclusion: The crude ethanolic leaf extract and its methanolic fraction of P. multifida showed the potential activity in antioxidant and anticancer activity. Rutin had a potent antioxidant activity while apigenin-7-O-β-D-glucopyranoside had a strong anticancer activity against the human breast adenocarcinoma cell line MCF-7.