RESUMEN
Objective To activate partial thromboplastin time (APTT) indicating the coagulation state in intrinsic coagulation system and the efficacy of anticoagulant drugs, and develop a bioassay method to quantify anticoagulating bioactivity in Chuanxiong Rhizome and related Chinese patent medicines for quality assessment. Methods Chuanxiong Rhizome powder was extracted in ethanol and water, respectively. The mixed extract was used as sample to quantify the level of extended APTT in vitro. The blood was taken from the heart of rabbit. The agglomerating time was determined after adding APTT reagent in plasma. The prolongation rate of APTT was chosen as biomarker for anticoagulating bioactivity. Sodium ferulate was chosen as reference. The amount of anticoagulating bioactivity was quantified in Chuanxiong Rhizome extract by the Amount reaction of parallel line analysis (2.2) method. Moreover, the amounts of anticoagulating bioactivity were quantified in the nine Chuanxiong Rhizome samples including Chuanxiong Rhizome raw materials, decoction pieces, and related Chinese patent medicines. Results Both sodium ferulate and Chuanxiong Rhizome extract showed significant anticoagulating bioactivity (P < 0.01). The reliability test for quantifying the level of anticoagulating bioactivity in solidum ferulate and Chuanxiong Rhizome extract was passed through, and the measured value was valid. The correlation coefficient was 0.995 5 (n = 5) between the concentration of solidum ferulate in the range of 1 — 5 mg/mL and their rates of anticoagulating bioactivity. The RSD for the amounts of anticoagulating bioactivity was 9.34% (n = 6) by six replicated tests with the confidence limit rate of 15.98% (n = 6). The amounts of anticoagulating bioactivity were significant difference among various types of Chuanxiong Rhizome samples, i.e. 5.431 — 7.620 U/g for five Chuanxiong Rhizome raw materials, 5.910 and 3.017 U/g for Chuanxiong decotion pieces and processed slice with yellow wine, 14.516 and 29.035 U/g for Tongmai Granules and Xuefu Zhuyu Pills. Conclusion The developed method can accurately quantify the level of anticoagulating bioactivity in Chuanxiong Rhizome raw materials, decoction pieces and related Chinese patent medicines, and assess their quality.