RESUMEN
Objective: To establish a quantitative analysis of multi-components by single mark (QMSA) for determining the contents of four anthraquinones (aurantio-obtusin, emodin, rhubarb, and physcion) from Cassiae Semen, and verify the feasibility of this method in quality control. Methods: The medicinal material of Cassiae Semen was used as the research object, emodin was used as reference, two kinds of correction methods were used to establish the relative correction factor (fk/s) of aurantio-obtusin, emodin, and physcion. The amount of each component was calculated, at the same time using the external standard method for determination of the three components, comparing the difference between calculated values of QMSA and measured value to validate the feasibility and accuracy of QMSA. Results: The relative correction factors of aurantio-obtusin, emodin, and physcion were established, without significant difference between the calculated values by QMSA and measured values. Conclusion: The experimental QMSA method can accurately determine the contents of the four anthraquinones in Cassiae Semen, and can be used in the multi-index evaluation in Cassiae Semen anthraquinones constituents.