RESUMEN
Objective To study the effects of ethanol extract of rhizoma phragmitis on liver glycogen content and glycogen synthetase (GS) in streptozotocin (STZ) induced diabetic mice. Methods The diabetic model mice were divided in-to model control group, high-dose group and low-dose group, 10 mice for each group. Another 10 normal mice were used as control group. The liver glycogen content was detected by histochemical staining of glycogen (PAS) method. The expression of GS mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR) and Western blot assays. Results After PAS staining the hepatic glycogen content decreased significantly in model control group, and which was significantly increased in low-dose group and high-dose group compared with that of model control group (P＜0.01). The hepatic glyco-gen content was the highest in high-dose group compared with that of other three groups. The levels of GS mRNA and GS protein were significantly lower in model control group than those of other three groups, which were significantly lower in low-dose group than those of high-dose group (P＜0.05). Conclusion There is a dose-dependent effect of ethanol extract of rhizoma phragmitis on liver glycogen in STZ induced diabetic mice, which may be related with the increased expression of liver glycogen synthetase.
RESUMEN
AIM: To provide the applied ground of the formulation of Rhizoma Phragmitis by setting up a fingerprint of Rhizoma Phragmitis. METHODS:We gained the extracts of Rhizoma phrapmitis by acetone extraction and assayed its extracts by means of HPLC, with a mobile phase of water (1% acetic acid)-water(5% acetonitrile) on inertsil ODS-3 column. an ultraviolet detector was at 250 nm. RESULTS:Fingerprint was established on the basis of the relative retention value and the relative peak area. CONCLUSION: The method is of high sensitivity and good reproducibility.