RESUMEN
Cell cultures of adults bovine retinal pigment epithelium(RPE) were propagated from central and peripheral regions of the same eyes to study the topographical differences in cell growth and to compare the differences in growth rate between two areas. The results obtained were as follows: A regional variation in the morpholgy was observed between the RPE from central and that from peripheral regions. Retinal pigment epithelium from central region attached to culture dish more slowly(average 4 days) than those from peripheral region(average 3.5 days) The growth rate of retinal pigment epithelium declined with serial passage in culture. The growth rate of retinal pigment epithelium from peripheral region at the first generation was highest. And there was a statistical difference in growth rate with passing in generation(P<0.05). This study reveals that growth rate and cell activity of RPE from central region are lower than from peripheral region.
Asunto(s)
Adulto , Humanos , Técnicas de Cultivo de Célula , Degeneración Macular , Epitelio Pigmentado de la Retina , Retinaldehído , Pase SeriadoRESUMEN
The authors investigated the possibility of transplantion of cultured retinal pigment epithelial cell to normal pigmented rabbit retina. Focal retinal detachment was made in the pigment rabbit retina, and the cultured RPE cells were injected into subretinal space. The neural retina spontaneously rettached withim 6 days. At 4 weeks after tranplantation, eyes were enucleated and examined with light-microscopy and electron-microscopy. The transplanted RPE cells were proliferated with multilayer in electronmicroscopic finding, and the tight-junction was found between proliferative RPE cells. The outer segment and nucleus of photoreceptor cell were well preserved in microscopic finding. As a result, the cultured RPE cells can be sucessfully transplanted to normal pigmented rabbit retina, and photoreceptor cell was not changed after transplantation.
Asunto(s)
Células Epiteliales , Células Fotorreceptoras , Retina , Desprendimiento de Retina , Epitelio Pigmentado de la Retina , RetinaldehídoRESUMEN
The retinal pigment epithelium(RPE) is one of the most important cell layers in the visual system and is vital to the integrity of the rod and cone outer segments. It has many complex functions, such as absorption of light, storage and conversion of vitamin A esters and formation of acid- mucopolysaccharide complex. As serum in the vitreous cavity stimulates the migration and proliferation of RPE cells, serum is necessary to grow RPE cells in culture. Bovine RPE cells were cultured with various concentrations of fetal bovine serum(FBS 0%, 5%, 10%, 20%, 40%) to evaluate their effects on RPE cell growth. FBS stimulated the proliferation of bovine RPE ells in a dose-dependent manner, with maximal proliferation at 20% FBS. This proliferative effect, however, was suppressed at the highest concentration(40% FBS).