Suero de conejo con actividad de complemento para tipificación del complejo mayor de histocompatibilidad humano / Rabbit serum with complement activity to typify human major histocompatibility complex

En el ensayo de microlinfocitotoxicidad, el suero de conejo como fuente de complemento desempeña un rol esencial en la clasificación del complejo mayor de histocompatibilidad humano, tanto para la tipificación antigénica como en el cross-match linfocitario de la pareja donante-receptor antes de realizar los trasplantes. En este trabajo, se obtuvieron 3 lotes experimentales de dicho hemoderivado con óptimos indicadores de rendimiento, actividad biológica y estabilidad. El exanguíneo de los animales se realizó por la técnica de yugulación. El suero fue separado por centrifugación en condiciones ambientales y de temperatura controladas. Posteriormente, el producto se sometió a filtración esterilizante y se tomaron muestras para los ensayos de calidad. El estudio mostró que el producto conserva la actividad biológica al menos durante 18 meses y que los valores de citotoxicidad se encuentran dentro de los límites de aceptación para su empleo en los ensayos de histocompatibilidad pretrasplante

Microlinfocitotoxicity assay is one of the serological methods used to classify human major histocompatibility complex. In this technique the rabbit´s serum as complement source, plays an essential role in antigens determination and in lymphocytes cross match assay as necessary stage before the selection of the best donor-receiver pair to realize the organ transplant. Three experimental lots of normal rabbit serum were developed with excellent indicators of efficiency, biological activity and stability. Rabbit's blood was obtained through jugulating technique and serum was separated by centrifugation in controlled environment and temperature´s conditions. The sterilization process was performed by filtration and the samples were taken for quality´s control assays. Stability studies showed that the product kept the biological activity at least during 18 months after it was processed and cytotoxicity's values were adequate for its employment in histocompatibility pre-transplant assays

Radioimmunoassay of polypeptide hormones using immunochemically coated plastic tubes.

A method has been developed for immobilisation of antisera on fresh plastic tubes through an immunochemical bridge. This type of immobilisation has been shown to be more consistent than direct adsorption on plastic. Such immunochemically coated antisera on plastic tube has been used in the development of a noncentrifugation radioimmunoassay. This assay system has been found to be technically as sound as the conventional method.

An Effect of Anti-Testicular Rabbit Serum on Rat Testis / 대한비뇨기과학회지

Evidence of the antigenicity of testis and semen was first presented at the end of the last century. Landsteiner (1899), Metchnikoff (1900), and Metalnikoff (1900) demonstrated the induction of a spermotoxic antibody in animals sensitized with testicular homogenate or semen; this antibody was capable of immobilizing sperm cells. The earliest manifestation of homologous type of antisperm sensitization (Kennedy, 1924) was the immobilization of spermatozoa, and in some cases atrophy of germinal epithelium, following repeated injection of testicular homogenate or epidydimal sperm. Ryoo and Kim (1982) reported that spermatogenesis was adversely affected with degeneration and sloughing of germinal cells of the seminiferous tubules in the mice which were immunized with testis homogenate plus complete Freund's adjuvant. The purpose of this study was to observe the effect of antitesticular rabbit serum produced against rat testis on spermatogenesis in rat. The results were as follows: 1.Theseminiferous tubules showed mild to moderate impairment of spermatogenesis such as degeneration and exfoliation of germinal epithelium in all experimental groups. Intraluminal spermatozoa of seminiferous tubules were decreased in number. Interspaces of seminiferous tubules were wider than normal and were infiltrated with mononuclear cells with some hemorrhage. 2. Intraluminal spermatozoa of the epididymides were markedly decreased in number but immature sperm cells were observed much more often than in normal control group.