Radiosensitization of sodium glycididazole in esophageal cancer evaluated by 18F-FMISO PET/CT / 中华核医学与分子影像杂志

Objective To investigate the feasibility of sodium glycididazole(CMNa)on the radio-sensitization of esophageal cancer(EC)by 18F-fluoromisonidazole(FMISO)PET/CT.Methods A total of 60 patients with EC from December 2016 to December 2017 were enrolled prospectively and were divided into control group(n = 30;21 males,9 females;age:(56.6±10.0)years)and experimental group(n=30;20 males,10 females;age:(59.3±9.0)years)using completely randomized grouping design.Patients in the control group received radiotherapy alone,and those in the experimental group were treated with conventional radiotherapy and CMNa.All patients underwent 18F-FMISO PET/CT imaging 1 week before and after radiotherapy.The imaging results were visually and semi-quantitatively analyzed.The maximum standardized uptake value(SUVmax),tumor/muscle ratio(T/M),and hypoxia volume(HV)were calculated.Paired t test,two-sample t test and/x2 test were used to analyze the data.Results T/M and HV in the control group before and after radiotherapy were 3.92±0.57 vs 1.66±0.35,(1.84±0.31)vs(1.04±0.15)mm3,respectively;T/M and HV in the experimental group before and after radiotherapy were 4.01±0.68 vs 1.27±0.11,(2.01±0.22)vs(0.90±0.09)mm3,respectively.The primary tumor T/M,HV after radiotherapy in 2 groups were significantly lower than those before radiotherapy(t values:12.15-24.43,all P<0.05)and the amplitudes of T/M and HV in the experimental group were significantly higher than those in the control group(?T/M:2.77±0.60vs 2.25±0.49,?HV:(1.12±0.18)vs(0.81±0.26)mm3;t values:3.00 and 1.80,both P<0.05).Meanwhile,the local control rate of EC after 3 months in the experimental group was higher than that in the control group(80.0%(24/30)vs 53.3%(16/30);x2=4.80,P<0.05).Conclusion CMNa has the radiosensitizing effect on EC and the 18F-FMISO PET/CT can evaluate the radiosensitization effect.

Application of nanomaterials in enhancing internal radiotherapy of radionuclide for cancer / 中华核医学与分子影像杂志

The therapeutic efficiency of radionuclides for cancer is closely related to the accumula-tion level, residence time and distribution in tumor, and is also influenced by the radiation resistance and hypoxia microenvironment of tumor. Nanomaterials, with a series of characteristics including special dimen-sion effect and functionally modifiable surface, can not only work as the isotopic carrier to transport nuclide into tumor, but also combine with chemical, thermal or photodynamic therapies to generate synergistic thera-peutic effect or destroy the hypoxic microenvironment of tumor to reduce radiation resistance, thus achieving the radiosensitization effect. This article reviews the application of nanomaterials in enhancing the therapeu-tic efficiency of radionuclide for cancer.

Evaluation of radiosensitivity enhancement by irisquinone with 18F-FETNIM microPET/CT imaging in breast cancer bearing nude mice / 中华核医学与分子影像杂志

Objective To investigate the effect of irisquinone (IR) on the radiosensitization of MDA-MB-231 breast cancer in nude mice using 18F-fluoroerythronitroimidazole (18F-FETNIM) microPET/ CT.Methods Thirty-two nude mice bearing MDA-MB-231 breast cancer were divided into 4 groups by random number table method (n =8 for each group):group A was treated with radiotherapy alone,group B was treated with radiotherapy and IR,group C was treated with IR alone,and group D was fed with distilled water.18F-FETNIM microPET/CT images were obtained to monitor the maximum standardized uptake value (SUVmax) of tumor before radiotherapy and 24 h after radiotherapy.The mice were sacrificed and tumor tissues were removed for HE staining.The expression of hypoxia inducible factor-1α (HIF-1α) was detected by immunohistochemical (IHC) method.Data were analyzed by paired t test,one-way analysis of variance,the least significant different t test and Pearson correlation analysis.Results There was no significant difference in SUVmax among the 4 groups before radiotherapy (1.429±0.090,1.430±0.076,1.445±0.071,1.432± 0.074;F=0.072,P＞0.05).At 24 h after radiotherapy,the SUVmax of group A and B decreased significantly (1.075±0.098,0.890±0.076;t values:12.888,33.217,both P＜0.05),and there was significant difference between group B and group A (t =4.197,P＜0.05).However,the SUVmax of group C and D increased significantly (1.617±0.090,1.644±0.063;t values:-11.009,-16.061,both P＜0.05).Pathological results showed that tumor cells in group A and B were reduced.IHC results showed that the expression of HIF-1α was lower in group B than others ((26.75±7.19)％;F=46.745,t values:2.898-9.743,all P＜ 0.05).The expression of HIF-1α was positively correlated with SUVmax(r =0.89,P＜0.05).Conclusion 18F-FETNIM microPET/CT could evaluate the radiosensitization effect of IR on MDA-MB-231 breast cancer in nude mice.

Novel drug targets in radiosensitivity of neoplasms / 国际肿瘤学杂志

Recent studies find a number of promising drug targets which can be applied for increasing tumor radiosensitivity in addition to normal tissue radioprotection,including oxygen free radical scavenger,drug targets in view of DNA damage repair and cell cycle,new targets inhibiting cell death,radioprotection mediated by growth factors,regulation of the cell signaling pathways,angiotensin-converting enzyme inhibitor.radiorestorative chemicals and so on.

Radiosensitization of artemisinin and its derivatives in tumors / 国际肿瘤学杂志

Artemisinin and its derivative is a kind of efficient,quick and low toxicity of anti-malarial drug.In recent years,we find that artemisinin drugs can not only against malaria,but also have pharmacological effects of immunosuppression,antiviral and anticancer.A number of studies have confirmed that artemisinin and its derivatives have the radiosensitization effects in nasopharyngeal carcinoma,cervical cancer,lung cancer and glioma,and their mechanisms are related to decreasing Weel protein expression,increasing Cyclin B1 protein expression,blocking G2-M phase and cell apoptosis.

Determination of content and related substances of radiosensitizer benzotriazine derivatives YABQ by HPLC / 军事医学

Objective To establish an HPLC method for determination of the content of radiosensitizer YABQ and its related substances.Methods Diamonsil C18 column (250 mm ×4.6 mm,5 μm) was used.The mobile phase consisted of methanol-0.1%formic acid (22∶78) and isocratic elution at a flow rate of 1.0 ml/min.The detection wavelength was 266 nm,the temperature of the column was 30℃, and the injection volume was 10 μl.Results The linear range of YABQ was 7-84 μg/ml, r=0.9992.The detection limit was 8 ng (S/N≥3), and the quantitation limit was 24 ng (S/N≥10). According to destructive sample processing, the separation coefficient between the peaks was above 1.5, indicating that this chromatographic method could meet the YABQ monitoring requirements.Conclusion Method validation and destructive testing show that both the precision and specificity are fine with this method, which could be used for quality control of YABQ and its related substances.

Caffeic Acid Phenethyl Ester Increases Radiosensitivity of Estrogen Receptor-Positive and -Negative Breast Cancer Cells by Prolonging Radiation-Induced DNA Damage / 한국유방암학회지

PURPOSE: Breast cancer is an important cause of death among women. The development of radioresistance in breast cancer leads to recurrence after radiotherapy. Caffeic acid phenethyl ester (CAPE), a polyphenolic compound of honeybee propolis, is known to have anticancer properties. In this study, we examined whether CAPE enhanced the radiation sensitivity of MDA-MB-231 (estrogen receptor-negative) and T47D (estrogen receptor-positive) cell lines. METHODS: The cytotoxic effect of CAPE on MDA-MB-231 and T47D breast cancer cells was evaluated by performing an 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) assay. To assess clonogenic ability, MDA-MB-231 and T47D cells were treated with CAPE (1 µM) for 72 hours before irradiation, and then, a colony assay was performed. A comet assay was used to determine the number of DNA strand breaks at four different times. RESULTS: CAPE decreased the viability of both cell lines in a dose- and time-dependent manner. In the clonogenic assay, pretreatment of cells with CAPE before irradiation significantly reduced the surviving fraction of MDA-MB-231 cells at doses of 6 and 8 Gy. A reduction in the surviving fraction of T47D cells was observed relative to MDA-MB-231 at lower doses of radiation. Additionally, CAPE maintained radiation-induced DNA damage in T47D cells for a longer period than in MDA-MB-231 cells. CONCLUSION: Our results indicate that CAPE impairs DNA damage repair immediately after irradiation. The induction of radiosensitivity by CAPE in radioresistant breast cancer cells may be caused by prolonged DNA damage.

Radiotherapy sensitization mechanisms of tumor necrosis factor-α / 国际肿瘤学杂志

Tumor necrosis factor-α (TNF-α)is a kind of cytokines with a wide range of biological activity,which is closely related to the occurrence and development of various diseases.Nowadays,a number of researchers use TNF-α as a radiation-sensitizing agent to evaluate the effect of radiation sensitivity of tumor cells.The scheme may have correlation with cell apoptosis,cell cycle,hypoxic cells,repair of DNA damage,etc.

18F-FDG PET/CT in evaluating the radiosensitizing effect of oleanolic acid / 中华核医学与分子影像杂志

Objective To evaluate the feasibility of 18F-FDG PET/CT in assessing the radiosensitizing effect of oleanolic acid (OA) in C6 rat glioma model,and to explore the possible mechanism of radiosensitizing effect of OA.Methods Thirty-two C6 glioma-bearing SD rats were divided into 4 groups by random number table:group A without OA and radiotherapy,group B with OA alone,group C with radiotherapy alone,group D with OA and radiotherapy.18F-FDG PET/CT was performed before radiotherapy,at 24 h and 7 d after radiotherapy.The tumor SUVmax was measured.All rats were sacrificed and tumor tissues were excised for HE and immunohistochemistry staining.Paired t test,one-way analysis of variance,LSD-t test and Spearman correlation analysis were performed to analyze the data using SPSS 13.0.Results There was no statistically significant difference in SUVmax among the 4 groups (SUVmax of groups A,B,C,D:5.252± 0.536,5.261±0.544,5.273±0.520,5.232±0.507) before treatment (F=0.008,P＞0.05).At 24 h postradiotherapy,SUVmax of groups C and D (4.766±0.511 and 4.403 ±0.486) decreased significantly (t =14..788,13.366,both P＜0.05);while groups A and B (5.680±0.635 and 5.763±0.689) increased significantly (t =-11.578,-8.651,both P＜0.05;F=10.550,P＜0.05).However,there was no statistically significant difference between groups C and D (t =1.453,P＞0.05).At 7 d post-radiotherapy,SUVmax of groups C and D decreased significantly (t =9.750,10.530,both P＜0.05);while SUVmax of groups A and B (t=-35.353,-6.884,both P＜0.05) increased significantly (F=97.691,P＞0.05).SUVmax of Group D decreased significantly compared with that of group C (t =5.329,P＞0.05).Less tumor cells and more areas of necrosis were observed in group D compared with those in other groups by HE staining.The expression of HIF-1α was lower in group D than that in other groups by immunohistochemistry staining.HIF-1α expression was positively correlated with SUVmax(r=0.853,P＜0.05).Conclusion 18F-FDG PET/CT has potential to evaluate the radiosensitizing effect of OA in vivo,and the radiosensitization of OA might be related to the down regulation of HIF-1α.

Clinical study on the radiosensitizer of esophageal carcinoma / 国际肿瘤学杂志

Radiosensitizer as a promising novel anti-tumor medicine has synergetic effects with radiotherapy,and can enhance the kill rate of tumor cells and radiotherapy effect.In recent years,due to the new theory and technology,the researches of radiosensitizer extend to different fields,from the traditional DNA target sensitizer to new radiosensitizer including nano-particles.Consequently,radiosensitizer combined with radiotherapy will become a new and effective treatment strategy for esophageal carcinoma to enhance the therapeutic effect of esophageal carcinoma.

Tumor Growth Suppression and Enhanced Radioresponse by an Exogenous Epidermal Growth Factor in Mouse Xenograft Models with A431 Cells / Journal of the Korean Cancer Association, 대한암학회지

PURPOSE: The purpose of this study was to evaluate whether an exogenous epidermal growth factor (EGF) could induce anti-tumor and radiosensitizing effects in vivo. MATERIALS AND METHODS: BALB/c-nu mice that were inoculated with A431 (human squamous cell carcinoma) cells in the right hind legs were divided into five groups: I (no treatment), II (EGF for 6 days), III (EGF for 20 days), IV (radiotherapy [RT]), and V (RT plus concomitant EGF). EGF was administered intraperitoneally (5 mg/kg) once a day and the RT dose was 30 Gy in six fractions. Hematoxylin and eosin (H&E) stained sections of tumor, liver, lung, and kidney tissues were investigated. Additionally, tumors were subjected to immunohistochemistry staining with caspase-3. RESULTS: EGF for 6 days decreased tumor volume, but it approached the level of the control group at the end of follow-up (p=0.550). The duration of tumor shrinkage was prolonged in group V while the slope of tumor re-growth phase was steeper in group IV (p=0.034). EGF for 20 days decreased tumor volume until the end of the observation period (p < 0.001). Immunohistochemistry revealed that mice in group V showed stronger intensity than those in group IV. There were no abnormal histological findings upon H&E staining of the normal organs. CONCLUSION: EGF-induced anti-tumor effect was ascertained in the xenograft mouse models with A431 cells. Concomitant use of EGF has the potential role as a radiosensitizer in the design of fractionated irradiation.

Research Progress in the mechanism of histone deacetylase inhibitors as radiosensitizers / 国际肿瘤学杂志

One leading research target of modern tumor radiotherapy is to increase radiosensitization of tumor and improve curative effect of radiotherapy.Histone deacetylase inhibitors are epigenetic drugs that can play a part in radiosensitization through means of induction of apoptosis,inhibition of repair of DNA doublestrand breaks,cell cycle arrest,improvement of tumor cell hypoxia and increase of reactive oxygen species.There is an urgent need to develop biomarkers based on these pathways,which can promote clinically individualized treatment.

The role of 18F-FDG PET/CT in evaluation of radiosensitivity enhancement by irisquinone / 中华核医学与分子影像杂志

Objective To evaluate the value of 18F-FDG PET/CT in assessing radiosensitivity enhancement by irisquinone (IR) on rabbit xenografted VX2 lung tumor models.Methods Twenty-four tumor-beating rabbits were randomly divided into 3 groups (8 rabbits/group):group A with radiotherapy alone,group B with combined radiotherapy and IR,and group C without radiotherapy (the control group).18F-FDG PET/CT imaging was performed before radiotherapy and 24 h and one week after radiotherapy.The tumor SUVmax on delayed imaging was calculated in all rabbits.Two rabbits in each group were sacrificed after PET/CT imaging.HE staining was used to assess the differences in cancer cells among groups.Paired t test,one-way analysis of variance and Kaplan-Meier analysis were performed to analyze the data using SPSS 13.0.Results Before radiotherapy,the tumor SUVmax of all the 24 rabbits on standard and delayed imaging were 2.200 ± 0.761 and 3.162 ± 0.833 (t =-5.582,P ＜ 0.01).At 24 h post-radiotherapy,the delayed SUVmax of groups A,B and C were 2.614 ± 0.654,2.349 ± 0.869 and 5.663 ± 1.144,respectively.The differences between pre-radiotherapy and 24 h post-radiotherapy were statistically significant in all three groups (t =2.527,3.620,11.011,all P ＜0.05).One week after radiotherapy,the delayed SUVmax of groups A,B and C were 3.625 ± 1.064,3.058 ±0.850 and 7.424 ± 1.751,respectively.The differences among groups A,B and C were statistically significant (tA∶ B =2.652,tA∶C =3.799,tB∶C =4.366,all P ＜0.05).The cancer cells of group B were fewer than those of groups A and C by pathological findings,which was consistent with 18F-FDG PET/CT results.The survival times of groups A,B and C were (62.375 ±4.534),(69.000 ±4.660) and (54.125 ±5.276) d,respectively.Kaplan-Meier survival curves revealed better survival of group B as compared to groups A and C,respectively (Log-rank,x2 =7.355,16.943,both P ＜ 0.01).Conclusion 18 F-FDG PET/CT is able to evaluate the effect of irisquinone on tumor radiosensitivity enhancement.

Sequence-Dependent Radiosensitization of Histone Deacetylase Inhibitors Trichostatin A and SK-7041 / Journal of the Korean Cancer Association, 대한암학회지

PURPOSE: This preclinical study is to determine whether the capacity of histone deacetylase (HDAC) inhibitors to enhance radiation response depends on temporal sequences of HDAC inhibition and irradiation. MATERIALS AND METHODS: The effects of HDAC inhibitors trichostatin A (TSA) and SK-7041 on radiosensitivity in human lung cancer cells were examined using a clonogenic assay, exposing cells to HDAC inhibitors in various sequences of HDAC inhibition and radiation. We performed Western blot of acetylated histone H3 and flow cytometry to analyze cell cycle phase distribution. RESULTS: TSA and SK-7041 augmented radiation cell lethality in an exposure time-dependent manner when delivered before irradiation. The impact of TSA and SK-7041 on radiosensitivity rapidly diminished when HDAC inhibition was delayed after irradiation. Radiation induced the acetylation of histone H3 in cells exposed to TSA, while irradiation alone had no effect on the expression of acetylated histone H3 in TSA-naive cells. Preirradiation exposure to TSA abrogated radiation-induced G2/M-phase arrest. When delivered after irradiation, TSA had no effect on the peak of radiation-induced G2/M-phase arrest. CONCLUSION: TSA and SK-7041 enhances radiosensitivity only when delivered before irradiation. Unless proven otherwise, it seems prudent to apply scheduling including preirradiation HDAC inhibition so that maximal radiosensitization is obtained.

Radiosensitizing effect of natural active product on tumor / 国际肿瘤学杂志

It has been indicated that there are a large number of natural active compounds generating the radiosensitizing effect on tumor.According to diverse molecular mechanism,these compounds may enhance the radiotherapeutic efficacy via enhancing the radiosensitivity of tumor.Studying the radiosensitization mechanism and classifying the natural compounds with the potentiality of development into clinical radiosensitizers can provide certain ideas for further research and development of natural radiosensitizers.

Effect of irradiation-induced miR-34a expression on irradiation-sensitivity of cells / 第二军医大学学报

Objective To investigate the relationship between miR-34a expression and the irradiation-sensitivity of cells and tissues. Methods RT-PCR was used to detect the miR-34a expression in different cells and tissues before and after irradiation; flow cytometry was employed to examine the apoptosis of cells and MTT assay was used to observe the cell viability. The U87MG tumor cells were transfected with miR-34a mimics to promote miR-34a expression and the cell viability was observed 24 h later. The HEK293 renal epithelial cells were transfected with miR-34a inhibitors to decrease miR-34a expression and the cells apoptosis was observed 24 h later. Results The expression of miR-34a was higher in highly irradiation-sensitive cells than in lowly irradiation-sensitive cells before irradiation; the increase of miR-34a expression was greatly higher in highly irradiation-sensitive cells than in lowly irradiation-sensitive cells 24 h after irradiation. The up-regulation of miR-34a decreased the viability of U87MG tumor cells, and down-regulation of miR-34a decreased the apoptosis of HEK293 cells. Conclusion The irradiation-sensitivity of cells is posilively associated with miR-34a; cells with high irradiation-sensitivity have a greater up-regulation of miR-34a expression after irradiation. Increased miR-34a expression can noticeably increase the irradiation-sensitivity of U87MG tumor cells and decreased miR-34a expression has a irradiation prevention effect on normal HEK293 cells.

Role of STAT3 antisense nucleic acid in promoting radiosensitivity of A549 cells / 第二军医大学学报

Objective To investigate the influence of self-designed STAT3 antisense oligodeoxynucleotides (STAT3 ASODN) on irradiation sensitivity of lung adenocarcinoma. Methods Lung adenocarcinoma cells were exposed to different doses of irradiation (4, 8, 12, 16, and 20 Gy 60Co γ) after transfected with STAT3 ASODN. The survival rates of cells in each group were evaluated by CCK-8 assay. Early apoptosis of cells was observed by FACS. Local irradiation models were established with tumof-bearing mice. STAT2 ASODN was intratumorally injected for two weeks at a dose of 15 mg/kg, and 2 h alter injection the animais were locally irradiated with 60Co γ(totally 20 Gy, 2 Gy each time, 5 times each week for 2 weeks, with a irradiation rate of 1 Gy/min). The following parameters were observed, including the tumor size, delay of tumor growth, relative growth rate, growth inhibition rate, q value, and tumor mass. Results In vitro experiment showed that compared with irradiation alone, combination of irradiation with STAT3 ASODN signiflcantly decreased the survival rate and increased the early apoptosis rate of A549 celis. In vivo experiment found that combination of STAT3 ASODN with local irradiation greatly inhibited the growth rate of lung adenocarcinoma xenografts (q= 1.27). Conclusion STAT3 ASODN not only possess chemotherapeutic effect, but also can improve the sensitivity of tumor cells, making it a promising agent for future research and clinical application.

In vivo study of radiosensitization by Topotecan on nasopharyngeal carcinoma / 中国耳鼻咽喉头颈外科

OBJECTIVE To study the radiose-nsitization by Topotecan on human nasopharyngeal carcinoma in nude mice. METHODS ①To study the maximum tolerance dose of TPT and detect the effective rate of TPT and RT on nude mice. ② Plan of radiosensitization practice:53 nude mice xenografts were distributed to 5 groups:RT 20 Gy group,RT 40 Gy group,TPT 12.5 mg/kg group,TPT 12.5 mg/kg+RT 20 Gy group and the controlgroup. After treatment,the volume of tumors were measured every 3 days in order to value the effective rate [complete remission(CR) + partial remission(PR) ]and regrowth delay time(TGD) and to fit the growth curve. RESULTS This study showed that the effective rates had significant difference among RT20 Gy+TPT 12.5 mg/kg group,RT20 Gy group and TPT12.5 mg/kg group,while that of RT20 Gy +TPT 12.5 mg/kg group and RT40 Gy group had no statistical difference. SER reached to 1.34. CONCLUSION Topotecan has been shown a radiosensitizing effect on human nasopharyngeal carcinoma in vivo.

The Effect of Combination Therapy with Radiation and Selective Cyclooxygenase-2 Inhibitor on Xenografted Human Oral Cavity Squamous Cell Carcinoma in Nude Mice / 대한이비인후과학회지

BACKGROUND AND OBJECTIVES: It is known that tumor cells over-expressing COX-2 have resistance to many anticancer treatments. Among those treatments, along with surgery and chemotherapy, radiation therapy plays a significant role in the treatment of head and neck cancer. However, radiosensitivity of each cancer varies according to cancer types. Especially, the cancer over-expressing COX-2 is reported to have higher radioresistance to radiation therapy. The purpose of this study is to evaluate the effect of selective COX-2 inhibitor when combined with the radiation therapy, and to assess the possibility of clinical application of the selective COX-2 inhibitor for radiation therapy in the head and neck cancer. MATERIALS AND METHOD: The human oral cavity squamous carcinoma cells were cultured and xenografted in 40 athymic nude mice (1 x 10(7), left thigh, subcutaneous injection) and the mice were divided into 4 groups: the control group (10 mice), the radiation therapy group (10 mice, Group A), the Meloxicam injection group (10 mice, Group B), and the combination therapy group with radiation and Meloxicam (10 mice, Group C). The tumor volume was measured on every five days during the treatment and the tumor specimen was taken for immunohistochemical staining when the treatment was finished. The mean tumor volume, the apoptosis index and the proliferation index were measured. RESULTS: In the combination therapy group (Group C), the tumor growth rate was decreased compared to the radiation therapy group (Group A). Also, according to the result of the apoptosis index and the proliferation index measured using immunohistochemical staining, the combination therapy group presented a higher apoptosis index but a lower proliferation index than other groups. CONCLUSION: Meloxicam, selective COX-2 inhibitor, improves the efficacy of the radiation therapy for the human oral cavity squamous carcinoma and this effect was due to apoptosis modulation by selective COX-2 inhibitor.

Observations on short-term uherapeutic effects of glycididazole combined with three-dimensional conformal radiotherapy for elderly patients with lung cancer / 解放军医学杂志

Objective To explore the role of glycididazole(CMNa) in enhancing radiosensitivity to three-dimensional conformal radiotherapy(3D-CRT) and its safety in elderly patients with lung cancer.Methods Twenty-seven elderly patients with lung cancer,which had been confirmed by pathological examination,were treated with radiosensitizer glycididazole(CMNa) and 3D-CRT.Diluted with 100ml saline solution,800mg/m2 glycididazole(CMNa) was intravenously injected within 30 minutes,and 3D-CRT was performed within 3 hours.3D-CRT was composed of 6MeV liner accelerator at a dose of 5.0-6.0 Gy/fraction for 3 fractions/week with a total dosage of 40-42Gy/3-4weeks.Treatment plan was evaluated by dose volume histogram(DVH) to ensure peripheral normal tissue and sensitive tissue to receive the dose within a permitted extent.Results Seventeen cases(63%) showed complete responses(CR),and 10 cases(37%) showed partial responses(PR),theretore the total response rate was 100%(27/27).No patients suffered from obvious adverse effects.Conclusions With significant ability to enhance radiosensitivity,glycididazole combined with 3D-CRT afford an effective treatment for elderly patients with lung cancer.