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ABSTRACT Purpose: The purpose of this study was to investigate the vascular effects of photobiomodulation using a light-emitting diode on the chorioallantoic embryonic membrane of chicken eggs grouped into different times of exposure and to detect the morphological changes induced by the light on the vascular network architecture using quantitative metrics. Methods: We used a phototherapy device with light-emitting diode (670 nm wavelength) as the source of photobiomodulation. We applied the red light at a distance of 2.5 cm to the surface of the chorioallantoic embryonic membrane of chicken eggs in 2, 4, or 8 sessions for 90 s and analyzed the vascular network architecture using AngioTool software (National Cancer Institute, USA). We treated the negative control group with 50 μl phosphate-buffered-saline (pH 7.4) and the positive control group (Beva) with 50 μl bevacizumab solution (Avastin, Produtos Roche Químicos e Farmacêuticos, S.A., Brazil). Results: We found a decrease in total vessel length in the Beva group (24.96% ± 12.85%) and in all the groups that received 670 nm red light therapy (2× group, 34.66% ± 8.66%; 4× group, 42.42% ± 5.26%; 8× group, 38.48% ± 6.96%), compared with the negative control group. The fluence of 5.4 J/cm2 in 4 sessions (4×) showed more regular vessels. The number of junctions in the groups that received a higher incidence of 670 nm red light (4× and 8×) significantly decreased (p<0.0001). Conclusion: Photo-biomodulation helps reduce vascularization in chorioallantoic embryonic membrane of chicken eggs and changes in the network architecture. Our results open the possibility of future clinical studies on using this therapy in patients with retinal diseases with neovascular components, especially age-related macular degeneration.
RESUMO Objetivo: investigar os efeitos vasculares da foto-biomodulação com diodo emissor de luz utilizando membrana embrionária corioalantóide de ovos de galinhas em grupos com diferentes tempos de exposição e detectar as alterações morfológicas por meio de métricas quantitativas promovidas pela luz na arquitetura da rede vascular. Métodos: Um aparelho de fototerapia com diodo emissor de luz no comprimento de onda de 670 nm foi usado como fonte de fotobiomodulação. A luz vermelha foi aplicada a uma distância de 2,5 cm da superfície da membrana embrionária corioalantóide em 2, 4 ou 8 sessões de 90 s a arquitetura da rede vascular foi analisada por meio do software AngioTool (National Cancer Institute, USA). Usamos um grupo controle negativo tratado com 50 µL de solução salina tamponada com fosfato (PBS) pH 7,4 e um grupo controle positivo (Beva) tratado com 50 µL de solução de bevacizumabe (Avastin, Produtos Roche Químicos e Farmacêuticos S.A., Brasil). Resultados: Uma diminuição no comprimento total do vaso foi detectada para o grupo Beva (24,96 ± 12,85%), e para todos os grupos que receberam terapia de luz vermelha de 670 nm, 34,66 ± 8,66% (2x), 42,42 ± 5,26% (4x) e 38,48 ± 6,96% (8x) em comparação ao grupo controle. A incidência de 5,4 J/cm2 em 4 sessões (4x) mostrou vasos mais regulares. A redução foi mais intensa nos grupos que receberam maior incidência de luz vermelha de 670 nm (4x e 8x). Conclusão: A fotobiomodulação contribui para a redução da vascularização nos vasos da membrana embrionária corioalantóide de ovos de galinhas e mudanças na arquitetura da rede. Os achados deste experimento abrem a possibilidade de considerar um estudo clínico usando esta terapia em pacientes com doenças retinais com componentes neovasculares, especialmente degeneração macular relacionada à idade.
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China has recently proposed a countrywide myopia control strategy for children and adolescents due to the rapidly increasing incidence and early onset of myopia.Recent studies have proposed interventions and methods for myopia prevention and control, such as low-dose atropine and orthokeratology lens, while the current strategies are subject to cause inconvenience and ineligible risks of side effects.Although accumulating evidence has shown that increased time outdoors is effective in reducing the risk of myopia development, its effect is closely related to light exposure.Interventions to enhance time spent outdoors or further increase on light exposure to prevent myopia are pragmatically challenging.A multicenter randomized controlled trial (RCT) led by Zhongshan Ophthalmic Center, adopted repeated low-level red-light (RLRL) to deliver light on the retina directly for a much shorter duration of exposure but repeatedly, and further assessed the efficacy and safety of RLRL therapy in myopia control among children.Findings from the RCT showed promising efficacy of RLRL therapy in slowing myopia progression among school-age children with mild to moderate myopia, providing high-level evidence for RLRL therapy as a safe and efficacious strategy for reducing myopia progression.This paper summarized the findings from the RCT, discussed how to adopt such a treatment for myopia control in clinical practice, and further suggested eye care service providers to follow the research progress of RLRL therapy for myopia prevention and control.
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Objective:To observe the clinical effect of traditional Chinese medicine fumigation combined with red light therapy on diabetic peripheral neuropathy ( DPN) . Methods:Totally 90 patients with DPN were numbered and divided into three groups accord-ing to the admission time: the control group, the fumigation group, the combined treatment group with 30 ones in each. The three groups were performed blood glucose control to keep blood sugar stable. The control group was given the conventional nerve nutrition drugs, the fumigation group was given traditional Chinese medicine fumigation additionally, and the combined treatment group was giv-en herbal fumigation combined with red light treatment additionally. After the 2-week treatment, the clinical symptom scores and the conduction velocity improvement of motor nerve and sensory nerve were compared among the three groups in order to evaluate the clini-cal efficacy. Results:The clinical symptom scores clinical efficacuy and the conduction velocity improvement of motor nerve and senso-ry nerve in the fumigation group and the combined treatment group were all better than those in the control group with significant differ-ences (P<0. 05), and the clinical symptom scores in the combined treatment group were better than those in the fumigation group (P<0. 05), while as for the clinical total efficay and the nerve conduction velocity, the combined treatment group showed no significant difference with the fumigation group (P>0. 05). Conclusion:Fumigation combined with red light in the treatment of diabetic periph-eral neuropathy shows notable efficacy with improved symptoms and nerve conduction velocity, which is worthy of promotion.
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Objective:To observe the clinical effect of traditional Chinese medicine fumigation combined with red light therapy on diabetic peripheral neuropathy ( DPN) . Methods:Totally 90 patients with DPN were numbered and divided into three groups accord-ing to the admission time: the control group, the fumigation group, the combined treatment group with 30 ones in each. The three groups were performed blood glucose control to keep blood sugar stable. The control group was given the conventional nerve nutrition drugs, the fumigation group was given traditional Chinese medicine fumigation additionally, and the combined treatment group was giv-en herbal fumigation combined with red light treatment additionally. After the 2-week treatment, the clinical symptom scores and the conduction velocity improvement of motor nerve and sensory nerve were compared among the three groups in order to evaluate the clini-cal efficacy. Results:The clinical symptom scores clinical efficacuy and the conduction velocity improvement of motor nerve and senso-ry nerve in the fumigation group and the combined treatment group were all better than those in the control group with significant differ-ences (P<0. 05), and the clinical symptom scores in the combined treatment group were better than those in the fumigation group (P<0. 05), while as for the clinical total efficay and the nerve conduction velocity, the combined treatment group showed no significant difference with the fumigation group (P>0. 05). Conclusion:Fumigation combined with red light in the treatment of diabetic periph-eral neuropathy shows notable efficacy with improved symptoms and nerve conduction velocity, which is worthy of promotion.