Molecular characterization and genetic diversity of different genotypes of Oryza sativa and Oryza glaberrima

Background: Availability of related rice species is critical for rice breeding and improvement. Two distinct species of domesticated rice exist in the genus Oryza: Oryza sativa (Asian rice) and Oryza glaberrima (African rice). New rice for Africa (NERICA) is derived from interspecific crosses between these two species. Molecular profiling of these germplasms is important for both genetics and breeding studies. We used 30 polymorphic SSR markers to assess the genetic diversity and molecular fingerprints of 53 rice genotypes of O. sativa, O. glaberrima, and NERICA. Results: In total, 180 alleles were detected. Average polymorphism information content and Shannon's information index were 0.638 and 1.390, respectively. Population structure and neighbor-joining phylogenetic tree revealed that 53 genotypes grouped into three distinct subpopulations conforming to the original three groups, except three varieties (IR66417, WAB450-4, MZCD74), and that NERICA showed a smaller genetic distance from O. sativa genotypes (0.774) than from O. glaberrima genotypes (0.889). A molecular fingerprint map of the 53 accessions was constructed with a novel encoding method based on the SSR polymorphic alleles. Ten specific SSR markers displayed different allelic profiles between the O. glaberrima and O. sativa genotypes. Conclusions: Genetic diversity studies revealed that 50 rice types were clustered into different subpopulations whereas three genotypes were admixtures. Molecular fingerprinting and 10 specific markers were obtained to identify the 53 rice genotypes. These results can facilitate the potential utilization of sibling species in rice breeding and molecular classification of O. sativa and O. glaberrima germplasms.

Nuevos genotipos de arroz resistentes a la Piriculariosis obtenidos por cultivo de anteras / New blast resistant rice genotypes obtained by and anthers culture

En la Estación Experimental del Arroz Los Palacios, perteneciente al Instituto Nacional de Ciencias Agrícolas de Cuba (INCA), se efectuaron cruzamientos entre cuatro cultivares resistentes a la Piriculariosis y seis de buen comportamiento agronómico y las anteras de las plantas F2 fueron cultivadas in vitro para evaluar la formación de callos en tres medios líquidos: N6-1, N6-m y NL, así como la regeneración de plantas verdes y albinas, en el medio MS. Las dos primeras generaciones de las nuevas líneas obtenidas fueron evaluadas para caracteres agronómicos y la segunda generación, además, para resistencia frente a la Piriculariosis. Las líneas que combinaron resistencia a la Piriculariosis y buenos caracteres agronómicos fueron evaluadas en condiciones de infección natural, con alta presión del patógeno. La utilización de la técnica del cultivo de anteras mostró alta dependencia del genotipo y el medio de cultivo. Con el medio NL se lograron los valores más altos para la formación de callos. Se obtuvieron nuevos genotipos resistentes a la Piriculariosis y de alto rendimiento agrícola.

Crosses were made between four blast resistant and six rice varieties of good agronomic performance, at the Los Palacios Rice Research Station of the National Agricultural Sciences Institute of Cuba (INCA) and the anthers from F2 plants were in vitro culture using three liquid media: N6-1, N6m, and NL, for callus formation and after plants regenerations using MS medium. The first two generations of breeding lines were evaluated for agronomic characters and the second generations, also, for Blast resistant. The lines that combined resistance to Blast and good agronomic performance were evaluated under high pressure of natural Blast infection conditions. The success rate of anther culture was highly dependent on the genotype and culture media used. NL medium led to the highest callus formation values. In the process, new blast resistant and high yielding genotypes were obtained.

Desempenho de cultivares e linhagens de arroz de terras altas sob plantio direto e convencional / Upland rice cultivars and lineages performance under no-till and conventional tillage

Objetivou-se com este trabalho comparar o desempenho de cultivares e linhagens de arroz de terras altas sob plantio direto e convencional. Para tanto, foi conduzido em Lavras-MG um experimento constituído de dois ensaios: um no sistema plantio direto e outro no sistema convencional, nos anos agrícolas 2003/2004 e 2004/2005. Foram testadas 13 cultivares e linhagens de arroz de terras altas. Utilizou-se o delineamento experimental de blocos ao acaso, com três repetições. As variáveis analisadas foram: produtividade de grãos, altura de planta, floração e incidência de doenças. As cultivares e linhagens têm comportamento semelhante para produtividade de grãos nos dois sistemas de cultivo. No sistema plantio direto, há uma tendência de redução na altura de plantas e alongamento do ciclo da cultura, comparado ao sistema convencional. Concluiu-se que, além do sistema convencional, o sistema plantio direto também constitui alternativa viável para a cultura do arroz de terras altas.

This study aimed to compare the performance of upland rice cultivars and rice lineages under no tillage (no-till) and conventional planting system, and to verify the existence of differentiated responses to both tillage systems. Thus, an experiment constituted of two essays, no-till and conventional tillage system was conducted in Lavras, MG, in 2003/2004 and 2004/2005 growing seasons. Thirteen upland rice cultivars and lineages were tested. A randomized block design and three replicates were used. Grain yield, plant height, flowering and disease incidence were analyzed. Rice cultivars and lineages presented similar yield in grains in both tillage systems. In no-till system there was a reduction tendency in plant height, disease incidance and cycle prolongation, as compared to conventional system. Therefore, no-till system is a viable alternative for upland rice crop.