Study on the Mechanism of Tibetan Medicine Wuwei Shexiang Pills in the Treatment of “Grum bu ”Disease Based on“Ro Nus ZhurJes-Network Target-Molecular Docking ” / 中国药房

OBJECTIVE：To investigate t he p otential mechanism of Tibetan medicine Wuwei shexiang pills in the treatment of “Grum bu ”disease（i.e. rheumatoid arthritis ）. METHODS ：Referring to Sibu Yidian ，Drug Standard of the Ministry of Public Health（Tibetan Medicine ），Jingzhubencao，the constituent ，dose and flavor of Wuwei shexiang pills were collected ；vector structure model was built. From the aspects of six tastes ，three tastes after digestion and seventeen effects ，the properties of the formulation were analyzed ；the“formulation-drug property-disease ”network for the treatment of “Grum bu ”disease was constructed by using Gephi 0.9.2 complex network software. The effective components of Wuwei shexiang pills were screened by using the pharmacology analysis platform of TCM system and the database of organic small molecule biological activity. The target proteins corresponding to the active components were predicted by using BATMAN-TCM network pharmacology research platform ； the target proteins of “Grum bu ”disease were searched by ETCM database. On the basis of screening the common targets of the two，David 6.8 bioinformatics resource database was adopted to conduct gene ontology （GO） analysis and KEGG pathway enrichment analysis ；the network of Wuwei shexiang pills- “Grum bu ”- target-pathway was constructed by using Cytoscape 3.7.0 software，and the network topology analysis was carried out to screen the core targets. Using Glide score as evaluation index ， Maestro Version 11.1.011 software was used for molecular docking of above core targets with effective components of Wuwei shexiang pills. RESULTS & CONCLUSIONS ：Wuwei shexiang pills contains myrobalan ，Aconitum pendulum ，Aucklandia lappa ， Acorus calamus and artificial moschus ，and the highest dosage was myrobalan. The six tastes of this formulation were mainly bitter and sweet ；the three tastes after digestion were mainly bitter ；the seventeen effects were mainly blunt ，cool and heavy ，which were mainly used to treat twenty characteristics such as acute ，hot and light. In “formulation-property-disease”network，higher values of edge weight between seventeen effects and twenty characteristics were cooling effect-heat ， blunt effect-sharpness ， heavy effect-light，dilute effect-odor （edge weight values ≥430），etc. A total of 2 306 potential targets of effective components of Wuwei shexiang pills ，211 targets related to “Grum bu ”disease，32 common targets and 29 corresponding effective components were obtained. The results of GO analysis showed that 52 relative results were predicted ，the common targets mainly located in the extracellular area ，nucleus and other parts ，mainly including biological processes and molecular functions such as immune response，inflammatory response ，cytokine activity. The results of KEGG enrichment were significant in 31 pathways（P＜0.05）， involving TNF signaling pathway ，cancer pathway and other signaling pathways. There were 94 active components ，targets and pathway nodes in the network of Wuwei shexiang pills- “Grum bu ”-target-pathway，and 460 edges；TNF，FAS，IL6，IL10， FASLG，PTGS2 and IL 1B were the core targets of the network ， connected with effective components such as quinic acid ， thymol，dehydroepiandrosterone，norcaxone by Van Der Waals force ，hydrogen bond and hydrophobic interaction ，Pi-cation bond and other bonds ，so as to play a role in the treatment of “Grum bu ”disease.

Study on regularity of drug use in Tibetan medicine treating hypertension based on multi-center clinical medical records and "prescription-property-efficacy-disease" / 中国中药杂志

This study aimed to explore the rule of Tibetan medicine in clinical treatment of hypertension( k Hrag-rLung-stod-vtshangs) and analyze the characteristics of its prescriptions. One hundred and thirty-seven cases of hypertension treated Tibetan medicine were collected. Data mining,Gephi,Cytoscape and other methods and software were used to analyze the characteristics of Tibetan medicine. The results showed that there were 44 cases of r Lung-type hypertension in clinical medical records,while 57 cases of k Hrag-type hypertension. There were 103 treatment prescriptions. The frequency of these prescriptions covered Twenty-five Yuganzi Pills( 96 times),Seventy Pearl Pills( 80 times),Eight Chenxiang Pills( 75 times),and Sanwei Ganlu Powder( 62 times),and they were highly correlated,with confidence greater than 95%. There were 332 prescriptions involved in the prescriptions which is core prescription medicines. This study first proposed the dosage analysis method of Tibetan medicine prescription medicines,and obtained the more dosage of Chebulae Fructus,Phyllanthi Fructus,Aucklandiae Radix,Aquilariae Lignum Resinatum and so on. The correlation analysis of the prescription medicines found that Carthami Flos,Myristicae Semen,Bambusae Concretio Silicea,Caryophylli Flos,Amomi Fructus Rotundus led by Tsaoko Fructus had a high correlation and a confidence greater than 75%. These herbs were guaranteed when Tibetan medicine was used in combination. The key drugs for protecting viscera and regulating the three gastric fires. The prescription is mainly cold,blunt,sparse and rough. Its efficacy focuses on the pathogenesis of blood fever,blood stickiness and venous blockage caused by heat,sharpness,odor and stickiness. It mainly treats Tibetan medicine diseases such as k Hrag-r Lung-stod-vtshangs and k Hrag-vpel( polyemia). It is suggested that Tibetan medicine has a three-in-one invisible treatment principle of " clearing blood-heat,opening vessel and regulating stomach-fire" in the treatment of hypertension,which attributed to both cardiovascular function and gastrointestinal metabolic function. This may be a significant and invisible knowledge of Tibetan medicine in the treatment of hypertension.

Prescription Regularity of The Four Medical Tantras-Scientific Implications of Ro Nus ZhurJes / 中国实验方剂学杂志

Ro Nus ZhurJes theory is the core content of Tibetan medicine,it can be divided into "taste performance"(Ro Yi Nus Pa) and "essential performance"(Ngo Bovi Nus Pa),and "taste performance" is divided into six tastes,eight properties,three tastes after digestion,seventeen effects and so on."Essential performance" is divided into eight kinds,including odor efficacy,sources efficacy,color efficacy,et al.Form bearing relationships that "taste performance" and "essential performance",the butt welding joins the three-factors and twenty characteristics of disease,it is the theoretical basis of Tibetan medicine from collection and processing,from formula to finished product. It is also the main method for the evaluation of drug performance by Tibetan medicine.Many classic Tibetan medicine prescriptions are compatible under the guidance of Ro Nus ZhurJes.However,at present,the research of Tibetan medicine mainly concentrates on the chemical composition and pharmacological mechanism.It is seldom studied from the Ro Nus ZhurJes theory of Tibetan medicine.It is difficult to find the core rules and effect of Tibetan medicine.Therefore,based on the ancient literature of Tibetan medicine,this paper combs the theory of Tibetan medicine property,explains the six tastes,three tastes after digestion,and seventeen effects of Tibetan medicine function in three-factors,twenty characteristics and other diseases attributes,in order to provide theoretical reference for the study of drug properties of Tibetan medicine.