Descripción de la Morfología del Sistema de Canales Radiculares en Premolares Maxilares Mediante Tomografía Computarizada Cone Beam en una Población Chilena

El tratamiento endodóntico requiere detallados conocimientos sobre la morfología radicular. En premolares maxilares, se ha reportado variabilidad en el número de raíces y morfología del sistema de canales radiculares (SCR). La causa más frecuente de fracaso endodóntico son los conductos no tratados. Por lo tanto, una mejor compresión de la morfología del SCR y sus variaciones es crucial para el tratamiento endodóntico. El objetivo de esta investigación fue describir la frecuencia del número de raíces y morfología del SCR en premolares maxilares, mediante exámenes de tomografía computarizada Cone Beam (CBCT) en una población chilena. Se realizó un estudio observacional descriptivo de corte transversal donde se observó la morfología del sistema de canales radiculares de primeros y segundos premolares maxilares a través de exámenes CBCT, agrupándolos de acuerdo con la Clasificación de Vertucci. Se observaron 228 exámenes CBCT, donde se incluyeron 268 primeros premolares superiores y 233 segundos premolares maxilares. Se determinó que en primeros premolares maxilares un 56,3 % presentó una raíz, un 43,3 %, siendo más frecuente una morfología del SCR tipo IV. En los segundos premolares maxilares, se determinó la presencia de una raíz en el 95,7 % de los casos, siendo más frecuente una morfología del SCR tipo I. Estos resultados pueden ser de interés para endodoncistas, ya que conocer la morfología del SCR de premolares maxilares permite mejorar la planificación de la terapia endodóntica realizada en estos dientes.

Endodontic treatment requires detailed knowledge of root morphology. In maxillary premolars, variability in root number and root canal system (RCS) morphology has been reported. The most frequent cause of endodontic failure is non treated canals. Therefore, a better understanding of RCS morphology and its variations is crucial for endodontic treatment. The aim of this research was to describe the frequency of root number and SCR morphology in maxillary premolars, by means of Cone Beam Computed Tomography (CBCT) examinations in a Chilean population. A cross-sectional descriptive observational study was carried out in which the morphology of the root canal system of maxillary first and second premolars was observed through CBCT examinations, grouping them according to the Vertucci Classification. A total of 228 CBCT examinations were observed, which included 268 upper first premolars and 233 maxillary second premolars. It was determined that in maxillary first premolars 56.3 % presented one root, 43.3 %, being more frequent a SCR type IV morphology. In maxillary second premolars, the presence of one root was determined in 95.7 % of the cases, being more frequent a SCR type I morphology. These results may be of interest to endodontists, since knowing the morphology of the RCS of maxillary premolars allows improving the planning of endodontic therapy performed on these teeth.

Descripción de la morfología del sistema de canales radiculares del primer molar maxilar mediante tomografía computarizada cone-beam en una población chilena / Description of the morphology of the root canal system of the maxillary first molar using cone-beam computed tomography in a Chilean population

Describir la morfología del sistema de canales radiculares del primer molar maxilar en una población chilena mediante exámenes de tomografía computarizada Cone-Beam (CBCT). Se realizó un estudio observacional descriptivo en el cual se utilizaron los exámenes CBCT de pacientes que fueron atendidos en un Centro de Radiología Maxilofacial privado durante el período comprendido entre Enero y Diciembre del año 2018 en la ciudad de Temuco, Chile, los cuales fueron observados de manera independiente por dos investigadores previamente calibrados. Se observaron 199 exámenes CBCT. En la raíz mesiobucal,predominaron las morfologías tipo II, I y IV de Vertucci respectivamente, mientras que en la raíz distobucal y palatina predominó la morfología tipo I. El canal MB2 estuvo presente en el 62,3 % de los casos, con una prevalencia significativamente mayor en pacientes jóvenes. En la mayoría de los primeros molares maxilares de los habitantes de la ciudad de Temuco se observaron tres raíces separadas y la presencia de cuatro canales. Se determinó una alta frecuencia del canal MB2 en la raíz MB.

SUMMARY: The objective of this study was to describe the morphology of the root canal system of the maxillary first molar in a Chilean population through Cone-Beam computed tomography (CBCT) examinations. A descriptive observational study was carried out in which the CBCT examinations of patients who were treated in a private maxillofacial radiology center during the period between January and December 2018 in Temuco, Chile, were used which were observed independently by two previously calibrated researchers. In this analysis 199 CBCT exams were observed. In the mesiobuccal root, Type II, I and IV morphologies predominated respectively, while in the distobuccal and palatal root, Type I morphology predominated. The MB2 canal was present in 62.3 % of cases, with a higher prevalence in young patients. Three separate roots and the presence of four canals were observed in most of the maxillary first molars of the patients in Temuco. A high frequency of the MB2 canal was determined in the root MB.

Anatomical and morphological findings of the root canal of maxillary premolars and their prevalence: CBCT study in a Mexican population / Hallazgos anatómicos y morfológicos del conducto radicular de premolares maxilares y su prevalencia: estudio CBCT en una población mexicana

SUMMARY: An essential prerequisite to perform any dental procedure is a clear understanding and knowledge of dental anatomy and its possible variations. The root canal system is characterized for a complex morphology, which varies among populations, individuals in the same population and even in the same person. The aim of this study was to evaluate by CBCT the morphology, number, curvature, and length of roots of first and second maxillary premolars in a Mexican population. In this stud 1700 maxillary premolars were evaluated by CBCT scans of patients; the axial, sagittal and coronal sections were analyzed following the longitudinal axis of each tooth. As a result 51.60 % of the maxillary first premolars had a single root, 31.03 % had two roots, 16.29 % had root fusion, and 1.07 % had three roots. 22.3 % of the maxillary first premolars showed mesial curvature, 41.9 % had a distal curvature, and 35.7 % did not show any curvature; and the most prevalent configuration in maxillary first premolars was Type V. 88.9 % of the maxillary second premolars had a single root, 3.9 % had two roots, 6.9 % had root fusion, and 0.11 % had three roots; 37.59 % of the maxillary second premolars showed a mesial curvature and 62.40 % showed a distal curvature; and the most prevalent configuration in maxillary second premolars was Type I. The anatomy of the root canal system is extremely complex and has many anatomical configurations, these clinical situations must be considered previous to performing any endodontic treatment.

RESUMEN: Un requisito esencial previo a la realización de cualquier procedimiento dental es una clara comprensión y conocimiento de la anatomía dental y sus posibles variaciones. El sistema de canales radiculares se caracteriza por una morfología compleja, que varía entre poblaciones, individuos en una misma población e incluso en una misma persona. El objetivo de este estudio fue evaluar mediante CBCT la morfología, número, curvatura y longitud de raíces de primeros y segundos premolares maxilares en una población mexicana. En este estudio se evaluaron 1700 premolares maxilares mediante escaneos CBCT de pacientes; se analizaron los cortes axial, sagital y coronal siguiendo el eje longitudinal de cada diente. El 51,60 % de los primeros premolares maxilares tenían una sola raíz, el 31,03 % tenían dos raíces, el 16,29 % tenían fusión de raíces y el 1,07 % tenían tres raíces. El 22,3 % de los primeros premolares maxilares presentaban curvatura mesial, el 41,9 % presentaban curvatura distal y el 35,7 % no presentaban curvatura alguna; y la configuración más prevalente en primeros premolares maxilares fue el Tipo V. El 88,9 % de los segundos premolares maxilares tenían una sola raíz, el 3,9 % dos raíces, el 6,9 % fusión de raíces y el 0,11 % tres raíces; El 37,59 % de los segundos premolares maxilares presentaban curvatura mesial y el 62,40 % curvatura distal y la configuración más prevalente en segundos premolares maxilares fue el Tipo I. La anatomía del sistema de canales radiculares es extremadamente compleja y tiene muchas configuraciones anatómicas, estas situaciones clínicas deben ser consideradas antes de realizar cualquier tratamiento de endodoncia.

Antimicrobial activity of probiotics against endodontic pathogens:- A preliminary study

Introduction: The purpose of this study was to evaluate the antibacterial effectiveness of probiotics lactobacilli group and Bifidobacterium against Enterococcus faecalis and Candida albicans in both planktonic stage and biofilm stage. Materials and Methods: Phase 1 of the study was conducted by agar well diffusion method. About 0.5 ml of test pathogen culture was inoculated on 20 ml of molten agar and allowed to solidify. 4�circular wells of diameter 8� mm were punched in each poured plates and 150 ?l of diluted test samples were added to the wells. Phase 2 was deferred antagonism test, wherein purified culture of pathogen strain was streaked at right angle to the original producer growth and incubated at 37癈 for 24 h. Zone of inhibition was measured for both the phases. Phase 3 biofilm stage evaluation was conducted by mixing 9 ml of 30% poloxamer 407 and De Man, Rogosa and Sharpe (MRS) broth in a test tube with 500 ?l of either pathogen, together with 500 ?l of test probiotic strains and incubated (37癈, 48 h), followed by serially diluting the mixture by 1 ml into 9 ml sterile saline till 108 dilutions for evaluation of colony-forming unit/ml counts. Controls were endodontic pathogens in 30% poloxamer with MRS broth and no probiotics. Results: Results were evaluated and statistically analysed using one-way analysis of variance and unpaired t-test. In the planktonic stage, probiotics showed inhibitory activity against endodontic pathogens with valid statistical significance (P < 0.05), while there was no activity by deferred antagonism method. In biofilm stage, all three probiotics showed growth reduction for E. faecalis, while lactobacilli group showed reduction in C. albicans colonies. Conclusion: This preliminary study suggested that probiotics are effective for preventing the growth of endodontic pathogens in vitro. Poloxamer could be utilised as an ideal delivery vehicle for probiotics.

Anatomical morphology of the root and root canal system of the second permanent molar in the Uygur people / 口腔疾病防治

Objective @#To study the anatomical characteristics of the root and root canal system of the mandibular second permanent molar in the Uygur people and provide a reference for clinical practice.@*Methods @#A total of 125 mandibular second permanent molars were extracted from Uygur patients in hospitals in the Xinjiang Uygur Autonomous Region. Three-dimensional reconstruction was performed after micro-CT scanning. The number of root canals, the root canal type (Weine classification and Fan′s C-shaped canal classification) and the occurrence of lateral accessory canals were observed.@*Results @#A sex difference was not detected in the root number or root form (χ2 = 1.277, P = 0.259). The incidence of 2-rooted molars was 70.4% (n = 88); type 2-1 canals were most common in the mesial root, with an incidence of 29.5%, followed by type 1-1 and 2-2 canals (each with an incidence of 26.1%), and the distal root mostly had a type 1-1 canal (96.6%). The incidence of single-rooted molars was 28.8%, and the frequency of C-shaped (n = 28) and non-C-shaped (n = 8) single-rooted molars was 22.4% and 6.4%, respectively. A three-rooted molar was detected in one case. The incidence of accessory canals was 65.2%.@*Conclusion @# Uygur mandibular second molars are mainly composed of two roots, and the incidence of a single root and root canal fusion is low (including C-shaped canals). Two-rooted molars frequently have two mesial canals and one distal canal.

Mandibular first molar with C-shaped root canal system: a two-case report / 华西口腔医学杂志

The root and canal anatomy of the mandibular first molar is highly complicated. A mandibular first molar with a C-shaped root canal system is rare. This article describes two different cases of this system. Case 1 has a complete C-shaped fused root. The root canal is characterized by a mesial lingual root canal and a C-shaped fused root canal. Case 2 has a C-shaped fused root in the buccal region and a distal lingual root. The root canal is characterized by a type Ⅱ-Ⅰroot canal in the buccal region and a distal lingual root canal.

Assessment of Mesial Root Morphology and Frequency of MB2 Canals in Maxillary Molars using Cone Beam Computed Tomography / Determinación de la Morfología de la Raíz Mesial y la Frecuencia del Canal MB2 en Molares Maxilares usando Tomografía Computadorizada de Haz Cónico

The aim of this study was to assess the morphology of the mesial root canal system of maxillary molars and the frequency of MB2 canals using cone-beam computed tomography (CBCT). A total of 1374 teeth, first maxillary (1MS, n= 802) and second maxillary molars (2SM, n= 572) of 508 Chilean patients between 8 to 77 years were evaluated through CBCT. The mesiobuccal root was evaluated in all three thirds. Root canal morphology was classified according to Vertucci's method. Data were analyzed by Pearson's Chi-square and Cuzick trend tests. MB2 canal frequency in 1MS was 73.44% and in 2MS 42.48%. The most frequent morphology in 1MS and 2MS were Vertucci type II and I, respectively. No statistically significant association was found between frequencies and side or according to gender (P>0.05). A positive association was found as the age increased in both 1MS and 2MS (P<0.001 and P= 0.023, respectively). Given the anatomical complexity of the mesiobuccal root and the frequent presence of the MB2 canal, the clinician must assume the existence of two canals in this root. CBCT scanning is a good way to initially identify this canal in the different root thirds.

Determinar la morfología del sistema de canales de la raíz mesial de molares maxilares y la frecuencia del canal MB2 usando tomografía computadorizada de haz cónico (TCHC). Un total de 1374 dientes, primeros molares maxilares (1MS, n= 802) y segundos molares maxilares (2MS, n= 572) de 508 pacientes Chilenos entre 8 y 77 años fueron evaluados a través de la TCHC. Se evaluó la raíz mesiobucal en todos sus tercio. La clasificación de Vertucci fue utilizada para determinar la morfología del sistema de canales. Los datos fueron analizados con los test Chi-Cuadrado de Pearson y la prueba de tendencia de Cuzick. La frecuencia del canal MB2 para el 1MS fue 73,44% y el 2MS 42,48%. La morfología mas frecuente en el 1MS y 2MS fueron el tipo II y I de Vertucci, respectivamente. No se encontró asociación significativa entre la frecuencia y el lado o sexo (p>0,005). Una asociación positive fue encontrada a medida que aumentaba la edad en ambos 1MS y 2MS (p<0,001 y p= 0,023, respectivamente). Dada la complejidad de la anatomía de la raíz mesiobucal y la frecuente presencia del canal MB2, el clínico debe asumir la existencia de dos canales en esta raíz. La TCHC es una buena manera de identificar tempranamente el canal en los diferentes tercio radiculares.

Morphology of Mesiobuccal Root Canals of Maxillary First Molars: a comparison of CBCT scanning and Cross-sectioning

Abstract: The aim of this study was to evaluate the mesiobuccal root of maxillary first molars, according to the root canal configuration, prevalence and location of isthmuses at 3 and 6 mm from the apex, comparing cone-beam computed tomography (CBCT) analysis and cross sectioning of roots by thirds. Images of the mesiobuccal root of 100 maxillary first molars were acquired by CBCT and then roots were cross-sectioned into two parts, starting at 3 mm from the apex. Data were recorded and analyzed according to Weine's classification for root canal configuration, and Hsu and Kim's classification for isthmuses. In the analysis of CBCT images, 8 root canals were classified as type I, 57 as type II, 35 as type III. In the cross-sectioning technique, 19 root canals were classified as type I, 60 as type II, 20 as type III and 1 as type IV. The classification of isthmuses was predominantly type I in both CBCT and cross-sectioning evaluations for sections at 3 mm from the apex, while for sections at 6 mm from the apex, the classification of isthmuses was predominantly types V and II in CBCT and cross-sectioning evaluations, respectively. The cross-sectioning technique showed better results in detection of the internal morphology of root canals than CBCT scanning.

Resumo: O objetivo do presente estudo foi avaliar a raiz mésio-vestibular de primeiros molares superiores, de acordo com a configuração do canal radicular e com a prevalência e localização de istmos a 3 e a 6 mm do ápice, comparando a análise realizada em tomografia computadorizada de feixe cônico (TCFC) com a técnica de seccionamento transversal por terços. Foram obtidas imagens tomográficas das raízes mésio-vestibulares de 100 primeiros molares superiores, e em seguida, as raízes foram seccionadas em dois segmentos, iniciando nos 3 mm a partir do ápice. Os dados foram analisados de acordo com a classificação de Weine para configuração de canais radiculares, e de acordo com a classificação de Hsu e Kim para avaliação dos istmos. Na análise das imagens das TCFCs, 8 canais radiculares foram classificados como tipo I, 57 como tipo II, e 35 como tipo III. Na técnica de seccionamento transversal, 19 canais radiculares foram classificados como tipo I, 60 como tipo II, 20 como tipo III, e 1 como tipo IV. Na avaliação dos istmos, houve predominância do tipo I tanto na TCFC quanto na técnica de seccionamento transversal a 3 mm do ápice. Entretanto, a 6 mm do ápice, a classificação dos istmos foi predominantemente tipo V e II, na avaliação em TCFC e na técnica de seccionamento transversal, respectivamente. A técnica de seccionamento transversal demonstrou melhores resultados na detecção da morfologia interna dos canais radiculares avaliados do que a TCFC.

Comparison of two different methods of average working length determination in second molars and premolars / Comparación de dos métodos para determinar la longitud de trabajo promedio en segundos molares y premolares

Within the procedures involved in a successful endodontic treatment is the working length (WL) determination. The root canal end must be detected accurately, and a precise control of the WL must be maintained. There are several methods to determine WL. Researchers have published the average teeth length (ATL) to the human permanent dentition. These measurements are used as an anatomical reference. If the WL is evaluated using a radiographic image, it is accepted as clinical success if the limit of the canal sealing is 1 mm coronal to the root apex. One method to determine WL is based in substract 1 mm to ATL. Another method widely used, assert to achieve a more precise WL determination, through the use of an electronic apex locator (AL). Nevertheless, published measures of ATL are still used as anatomical reference, and are used to determine WL in the absence of an AL. The aim of this study was to determine the average WL of second molars and premolars with endodontic treatment indication using a PropexII® AL, and compare these measurements to WL determination using ATL method. A descriptive cross-sectional study with a non-random sample of consecutive cases was executed. Results showed that there were no statistically significant differences between the WL obtained with AL and ATL method in studied teeth, except mesio-buccal canal of second maxillary molars (19.94 mm average using AL, 1.54 mm greater than ATL method 18.40 mm; p= 0.002). The ATL method to determine WL could be used to determine the WL of second molars and premolars in studied population. Further research should be performed to determine if ATL method is safe and reliable to be used in absence of an AL or in patients where this instrument cannot be used.

Entre los procedimientos involucrados en un tratamiento endodóntico exitoso está la determinación de longitud de trabajo (LT). Esta debe mantenerse durante todo el tratamiento, y el extremo del canal radicular (CR) debe detectarse con precisión. Existen varios métodos para determinar LT; los investigadores han publicado la longitud total promedio (LTP) de la dentición permanente humana. Estas mediciones se utilizan como referencia anatómica. Si LT se evalúa usando una imagen radiográfica, se acepta como éxito clínico cuando el límite de la obturación radicular queda 1 mm coronal al ápice radicular. Un método para determinar LT se basa en restar 1 mm a la LTP. Otro método, que permite una determinación de LT más precisa, se realiza utilizando un localizador apical electrónico (LA). Pese a esto último, las medidas publicadas de LTP todavía se utilizan como referencia anatómica, para determinar LT en ausencia de un LA. El objetivo fue determinar LT promedio de los CR de segundos molares y premolares con indicación de endodoncia utilizando LA PropexII®, y comparar estas mediciones con la LT determinada utilizando el método de LTP. Se realizó un estudio descriptivo de corte transversal con una muestra no probabilística de casos consecutivos. No hubo diferencias estadísticamente significativas entre la LT obtenida con LA y el método LTP en los dientes estudiados, salvo en los canales mesio-bucales de segundos molares superiores (19,94mm promedio usando LA, 1,54 mm mayor que el método LTP: 18,40 mm; p= 0,002). El método para definir LT a través de LTP podría utilizarse para determinar LT de segundos molares y premolares en la población estudiada. Se requieren investigaciones posteriores para determinar si el método que utiliza LTP es confiable para ser utilizado en ausencia de un LA o en pacientes en los que este instrumento no pueda ocuparse.

Incidence of C-Shaped Root Canal Systems in Mandibular Second Permanent Molar and the Effect of One-Visit Root Canal Treatment / 天津医药

Objective To investigate the incidence of C-shaped root canal systems in mandibular second perma?nent molar of patients in the First People’s Hospital of Yunnan Province, and compare the success rate between one-and two-visit root canal treatment. Methods Two hundred and sixty mandibular second molars were recruited from the pa?tients in the department of endodontics of our hospital, and teeth with C-shaped root canal were categorized by using radio?graphic classification criteria and the modified Melton’s method. Teeth with C-shaped root canal systems were then random?ly divided into two groups to receive one-or two-visit root canal treatment, and success rates of 6-month post-operation were compared between two groups. Results Eighty out of 260 mandibular second molars exhibited C-shaped canals (30.77%). Radiographic examination showed that 25 teeth belonged to typeⅠ(31.25%), 32 teeth typeⅡ(40.00%) and 23 teeth typeⅢ(28.75%) in two groups. As for modified Melton’s method, the incidence rates were 21.25%, 48.75%, 16.25%and 13.75% for C1,C2,C3a and C3b, respectively, while C4 was not found. The success rates were 78.94% (30/38) and 81.08%(30/37) for A group and B group, respectively, with no statistically significant difference between two groups ( 78.94 vs 81.08, P>0.05). Conclusion Results indicate a frequent incidence of C-shaped root canal system of mandibular per?manent second molars in our hospital. One-visit root canal treatment is an effective way for treating C-shaped canals in man?dibular permanent second molar.

Mandibular second molar with type 1-2 canal of mesial root:Report of one case / 实用口腔医学杂志

The root canal system of mandibular second molar is complex.This article presents one case with type 1-2 mesial root canal of mandibular second molar.The canal was treated by warm gutta percha,and conformed by X-ray examination and dental operating microscopy.

Apical foramen morphology according to the length of merged canal at the apex

OBJECTIVES: The aim of this study was to investigate the relationship between the apical foramen morphology and the length of merged canal at the apex in type II root canal system. MATERIALS AND METHODS: This study included intact extracted maxillary and mandibular human premolars (n = 20) with fully formed roots without any visible signs of external resorption. The root segments were obtained by removing the crown 1 mm beneath the cementum-enamel junction (CEJ) using a rotary diamond disk. The distance between the file tip and merged point of joining two canals was defined as Lj. The roots were carefully sectioned at 1 mm from the apex by a slow-speed water-cooled diamond saw. All cross sections were examined under the microscope at x50 magnification and photographed to estimate the shape of the apical foramen. The longest and the shortest diameter of apical foramen was measured using ImageJ program (1.44p, National Institutes of Health). Correlation coefficient was calculated to identify the link between Lj and the apical foramen shape by Pearson's correlation. RESULTS: The average value of Lj was 3.74 mm. The average of proportion (P), estimated by dividing the longest diameter into the shortest diameter of the apical foramen, was 3.64. This study showed a significant negative correlation between P and Lj (p < 0.05). CONCLUSIONS: As Lj gets longer, the apical foramen becomes more ovally shaped. Likewise, as it gets shorter, the apical foramen becomes more flat shaped.

Análise radiográfica da trajetória do quarto canal no primeiro molar superior / Radriographic analysis of the trajectory of the fourth canal in first maxillary molar

O objetivo deste trabalho foi avaliar radiograficamente a trajetória do canal mesiopalatino em primeiros molares superiores, visando verificar a importância da análise radiográfica no tratamento desse grupo de dentes, tendo em vista a grande incidência de dois canais na raiz mesiovestibular. Foram utilizados neste estudo 65 primeiros molares superiores extraídos, doados por alunos da PUC/PR. Os dentes foram radiografados com películas radiográficas periapicais, com 20 graus de angulação na incidência disto-excêntrica e com posicionamento de limas tipo K nº 10 nos canais. A análise buscou distribuir a amostra de acordo com a classificação do sistema de canais radiculares proposta por Weine [7]. O resultado obtido foi: 47,7% (31) do tipo I; 21,5% (14) do tipo II; 12,3% (8) do tipo III; 18,5% (12) calcificados. Pode-se concluir que a análise cuidadosa da raiz mesiovestibular é importante para que se possa assegurar um bom prognóstico ao tratamento

The purpose of this study was to evaluate, by radiographic exam, the trajectory of the mesiopalatal canal in maxillary first molars, aiming at assessing the importance of the radiographic analysis in the treatment of these teeth; due to great incidence of two canals in the mesiovestibular root. Sixty-five extracted maxillary first molars were donated by students of PUC/PR and used in this study. Periapical radiographs of the teeth were taken at 20 degrees in the disto-eccentric incidence and positioning #10 K-type files into the canals. The analysis aimed at distributing the sample according to the classification of root canal system proposed by Weine [7]. The obtained result was: 47.7% (31) ­ type I; 21.5% (14) ­ type II; 12.3% (08) ­ type III and 18.5% (12) ­ calcified. It can be concluded that a careful analysis of the mesiovestibular root is important to guarantee a good prognostic of the treatment