RESUMEN
Objective:To optimize the extraction technology of gallic acid in the roots of Rubus chingii Hu from eastern China and establish an assay method. Methods:The content of gallic acid was determined by HPLC. Orthogonal design was used to investigate the influence of solid/liquid ratio,extraction time and concentration of hydrochloric acid on the yield of gallic acid. Results:A good linearity of gallic acid was within the range of 4. 082-40. 815 μg·ml-1(r=0. 999 9),and the average recovery was 101. 28%(RSD=1. 08%,n=6). The optimal technology was as follows:extracted with heating circumfluence in 4. 2% hydrochloric acid solution with solid/liquid ratio of 1: 15 for 2. 5h. Conclusion:The optimal extraction technology is simple and feasible,and the assay method is stable and reliable,which can be used in the extraction and determination of gallic acid in the roots of Rubus chingii Hu from eastern China.