Phytochemical and antioxidant activities of Rumex crispus L.in treatment of gastrointestinal helminths in Eastern Cape Province, South Africa

Objective:To evaluate the antioxidant activities and phytochemical content of the leaf and root extracts of Rumex crispus using the solvents extraction;methanol extract,ethanol extract,acetone extract (ACE),and water extract.Methods:Total flavonoids content,total phenolic content,and total proanthocyanidin were evaluated using spectrophotometric equivalents of the standards,quercetin,gallic acid and catechin respectively.The antioxidant activities of the plant extracts were determined using ABTS,DPPH,ferric reducing antioxidant power,total antioxidant capacity and nitric oxide scavenging assays.Results:The flavonoids and phenols contents of the extracts were in the range of (19.39 ± 4.08) to (526.23 ± 17.52) mg QE/g and (16.95 ± 12.03) to (240.68 ± 3.50) mg GAE/g,respectively.ACE of the leaf has the highest value of total flavonoids content (526.23 ± 17.52) mg QE/g while ACE of the root has the highest value of total phenolic content (240.68 ± 3.50) mg GAE/g.The highest content of total proanthocyanidin (645.38 ± 1.33) mg CE/g was in ACE of the root.Significant amounts of saponin and alkaloid were also present in the root and leaf extracts.All solvent fractions showed significant antioxidant activities (P ＜ 0.05) with ACE of the root having the highest scavenging value as shown in DPPH,ABTS,total antioxidant capacity,nitric oxide and ferric reducing antioxidant power (IC50 =0.014 mg/mL,＜0.005 mg/mL,0.048 mg/mL,0.067 mg/mL,and 0.075 mg/mL,respectively).Conclusions:In this study,the mean phytochemical content of the root of Rumex crispus is higher than that of the leaf and this may have contributed to its high antioxidant activities.This may also justify the frequent use of the root more than the leaves in traditional medicine for the cure of helminthic infections.

Phytochemical and antioxidant activities of Rumex crispus L. in treatment of gastrointestinal helminths in Eastern Cape Province, South Africa

Objective To evaluate the antioxidant activities and phytochemical content of the leaf and root extracts of Rumex crispus using the solvents extraction; methanol extract, ethanol extract, acetone extract (ACE), and water extract. Methods Total flavonoids content, total phenolic content, and total proanthocyanidin were evaluated using spectrophotometric equivalents of the standards, quercetin, gallic acid and catechin respectively. The antioxidant activities of the plant extracts were determined using ABTS, DPPH, ferric reducing antioxidant power, total antioxidant capacity and nitric oxide scavenging assays. Results The flavonoids and phenols contents of the extracts were in the range of (19.39 ± 4.08) to (526.23 ± 17.52) mg QE/g and (16.95 ± 12.03) to (240.68 ± 3.50) mg GAE/g, respectively. ACE of the leaf has the highest value of total flavonoids content (526.23 ± 17.52) mg QE/g while ACE of the root has the highest value of total phenolic content (240.68 ± 3.50) mg GAE/g. The highest content of total proanthocyanidin (645.38 ± 1.33) mg CE/g was in ACE of the root. Significant amounts of saponin and alkaloid were also present in the root and leaf extracts. All solvent fractions showed significant antioxidant activities (P < 0.05) with ACE of the root having the highest scavenging value as shown in DPPH, ABTS, total antioxidant capacity, nitric oxide and ferric reducing antioxidant power (IC

Environmental friendly alkaline sulfite anthraquinone-methonal (ASAM) pulping with Rumex crispus plant extract of woody materials.

ASAM with Rumex crispus extract organosolv pulping was developed by using 1,5-dihydroxy-3-methoxy-7-methyl-anthraquinone from Rumex crispus root, instead of anthraquinone. ASAM was also produced as a control pulping. Both pulps were made by handsheets from fast growing P.deltoides clone (Samsun p.clone), Robinia pseudoacacia L. and Pinus pinaster grown in Turkey for wood fibrous raw materials. The mechanical consisting tensile, bursting and tear values and optical values of ASAM handsheets yellowness, brightness and whiteness were compared to ASAM with Rumex crispus L. extracted. It is concluded that ASAM with Rumex crispus extract pulping suits well in the manufacturing of special papers.

Carbohydrase inhibition and anti-cancerous and free radical scavenging properties along with DNA and protein protection ability of methanolic root extracts of Rumex crispus

The study elucidated carbohydrase inhibition, anti-cancerous, free radical scavenging properties and also investigated the DNA and protein protection abilities of methanolic root extract of Rumex crispus (RERC). For this purpose, pulverized roots of Rumex crispus was extracted in methanol (80% and absolute conc.) for 3 hrs for 60degrees C and filtered and evaporated with vacuum rotary evaporator. RERC showed high phenolic content (211 microg/GAE equivalent) and strong 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging (IC50 = 42.86 (absolute methanol) and 36.91 microg/mL (80% methanolic extract)) and reduced power ability. Furthermore, RERC exhibited significant protective ability in H2O2/Fe3+/ascorbic acid-induced protein or DNA damage and percentage inhibition of the HT-29 cell growth rate following 80% methanolic RERC exposure at 400 microg/mL was observed to be highest (10.2% +/- 1.03). Moreover, methanolic RERC inhibited alpha-glucosidase and amylase effectively and significantly (P < 0.05). Conclusively, RERC could be considered as potent carbohydrase inhibitor, anti-cancerous and anti-oxidant.