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Objective:To explore the effects of long non-coding RNA SATB2-AS1 in the proliferation and apoptosis of cervical cancer cells as an endogenous competitive RNA to regulate miR-373-5p/BTG3 axis.Methods:qRT-PCR was used to detect the expression of LncRNA SATB2-AS1, miR-373-5p and BTG3 in paracancerous tissue and cancerous tissue of patients with CC. The interaction between LncRNA SATB2/miR-373-5p/BTG3 was then predicted and verified. The expression of SATB2-AS1 and miR-373-5p in cells was intervened, subsequently the CC cells were divided into different groups. The proliferation activity of cells in each group was detected by MTT, and the apoptosis of cells in each group was detected by flow cytometry.Results:qRT-PCR showed that the expression of SATB2-AS1 and BTG3 in cancer tissue and CC cells was significantly decreased, while the expression of miR-373-5p was significantly increased in cancer tissue and CC cells compared with paracancerous tissue and normal cervical cells.The targeting relationship between SATB2-AS1 and miR-373-5p was confirmed. Compared with NC group, overexpression of SATB2-AS1 inhibited cell proliferation butinduced apoptosis. Overexpression of miR-373-5p promoted cell proliferation, inhibited apoptosis, but this effect was partially saved by SATB2-AS1.Conclusion:Up-regulation of LncRNA SATB2-AS1 expression regulated the miR-373-5p/BTG3 axis and participated in the progression of cervical cancer, subsequently inhibited cancer cell proliferation but induced apoptosis.
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Objective@#To retrospectively analyse the genetic characteristics, diagnosis, treatment and prognosis of special AT-rich binding protein 2 (SATB2)-associated syndrome.@*Methods@#Clinical data of one case of SATB2-associated syndrome diagnosed in Children′s Hospital Affiliated to Zhengzhou University in January 2018, were collected including clinical test, treatment plan, follow-up outcomes. The clinical characteristics of SATB2-associated syndrome were analyzed, and literature review was conducted.@*Results@#The female proband, eight-year-old, were admitted with the clinical manifestations including epilepsy seizures, delayed language development, sparse hair, long face, prominent forehead, long nose, lower eyelid cleft oblique, low ear, smooth philtrum, small mandible, sparse teeth arrangement, and lack of some teeth. The intelligence quotient score was 49. The brain magnetic resonance imaging showed myelinated dysplasia. Long-range video-electroencephalography showed spike-wave activity, slow spike-wave discharges in the bilateral middle and posterior temporal regions. The trio whole exome sequencing (trio WES) test showed that the proband carried a heterozygous nonsense mutation c.1300 C>T (p.Gln434Ter) in the SATB2 gene, and the muation was de novo comfirmed by pedigree analysis. Thirty-seven literatures relevant to SATB2-associated syndrome, from January 1989 to June 2019, were retrieved. Threre were 23 overseas literatures and one domestic report, including a total number of 158 cases. There were 49 missense mutations, 38 nonsense mutations, 32 frameshift mutations, seven splicing-site mutations, six translocation mutations, one insertion mutation, 22 gene-deletions and three gene-duplications. Among the 158 reported cases, 90 were male and 62 female, sex was not described in six cases. One hundred and fifty-eight (100.0%) patients had mental retardation, 44 (30.6%) with growth retardation, 107 (84.3%) with facial deformities, 70 (45.5%) with cleft palate, 135 (98.5%) with dental abnormalities, 66 (43.4%) with language retardation, 29 (20.0%) with epileptic seizures, and 50 (46.3%) with neuroimaging abnormalities.@*Conclusions@#The main clinical manifestations of SATB2-related syndromes are severe developmental retardation, low intelligence, delayed language development, language deficiency, high palatal arch and cleft palate, rare epilepsy seizures, dental anomalies and scant hair. The study identified a novel nonsense mutation c. 1300 C>T (p. Gln434Ter) in the SATB2 gene, which is responsible for the development of SATB2-associated syndrome.
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Objective To investigate the expression of SATB2 and P53 protein in colorectal cancer and to analyze their relationship with the clinical pathologic characteristics. Methods The expression of SATB2 and P53 protein in 30 normal colorectal tissues and 120 colorectal cancer tissues were detected by immunohistochemical method,then analyzed their correlation. Results The expression of SATB2 in colorectal cancer was lower than that in normal colorectal tissues(P<0.05);and its expression correlated with the tumor size,differentiation,inva-sion,depth,lymph node metastasis and TNM stages(P<0.05),while not with age and gender.The expression of P53 in colorectal cancer was significantly higher than that in normal colorectal tissues(P<0.01),and its expres-sion correlated with differentiation,invasion depth and TNM stages(P<0.05),while not with age,gender,tumor size and lymph node metastasis. The expression of SATB2 and P53 in colorectal cancer have negative correlation (P < 0.01). Conclusion Compared with in normal colorectal tissues,the expression of SATB2 declined and the expression of P53 increased in colorectal cancer,while they have negative correlation. The expression levels of SATB2 and P53 protein have important significance to evaluate the prognosis of colorectal cancer.
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Krukenberg tumors mostly occur after 40 years. Metastatic ovarian tumors in young age are very rare and reported to be 2% of all the cases. Thirty percent of all ovarian neoplasms occurring during childhood and adolescence are malignant. A 25-year-old woman, parity- 2, presented with abdominal distension, pain in abdomen and amenorrhea. On examination, 18 weeks lump was palpable, fi rm to hard in consistency, non-tender and mobile. On ultrasonography bilateral ovarian tumors were reported, without any peritoneal free fl uid. Total abdominal hysterectomy with bilateral salpingo-oophorectomy was performed. Microscopic examination revealed signet ring cells with glandular differentiation, diffusely invading the ovarian parenchyma. Tumor cells exhibited strong, diffuse immunopositivity for CEA with focal strong immunopositivity for CK7 and CK20 and immunonegativity for SATB2. Diagnosis of Krukenberg tumor was made. Endoscopic biopsy confi rmed the diagnosis of adenocarcinoma stomach. This case is reported because of its rarity in younger age group.