Metabolism of broilers subjected to different lairage times at the abattoir and its relationship with broiler meat quality / Metabolismo de frangos de corte submetidos a diferentes tempos de espera no abatedouro e sua relação com a qualidade da carne

An investigation was made into the effects of different lairage times and the position of chicken crates during transport to the slaughterhouse on the biochemical and hematological profile and physical parameters of broilers, such as color and pH of their breast meat. The treatments were defined by the animals slaughtered after 0, 2, 4 and 6 hours of lairage time at the slaughterhouse, transported in crates located in the top and bottom layers of the truck. It was found that increasing the lairage time at the slaughterhouse to over two hours reduced the number of lymphocytes and increased the heterophil/lymphocyte (H/L) ratio and the basophil count in the hemogram. In addition, lactate dehydrogenase (LDH) activity and cholesterol levels increased and plasma triglyceride and glucose levels decreased. The position of the crates in the truck altered the creatine kinase (CK) activity, and the highest enzyme activity was found in birds transported in the top layer of crates in the truck. Furthermore, the long lairage time in the slaughterhouse increased the pH and the value of a* (redness value) and decreased the lightness value of breast fillets. The interaction significant between 4 and 6 hours of lairage time and the position of the crate in the top layer of the truck favored the development of dark, firm, dry (DFD) meat.(AU)

Este trabalho objetivou a avaliação dos efeitos dos diferentes tempos de espera e da posição das caixas de transporte no caminhão sobre perfil bioquímico e hematológico, além de parâmetros físicos da carne, como cor e pH do peito, de frangos de corte. Os tratamentos foram definidos pelos animais abatidos com zero, duas, quatro e seis horas de espera no abatedouro, posicionados nas partes superior e inferior do caminhão. Como resultado, verificou-se que o aumento do tempo de espera no abatedouro, acima de duas horas, resultou em redução no número de linfócitos, elevação da razão heterófilos/ linfócitos (H/L) e de basófilos no hemograma. Houve aumento da atividade de lactato desidrogenase (LDH), dos níveis de colesterol e redução de triglicerídeos e glicose no plasma. O posicionamento das caixas na parte superior da carroceria do caminhão elevou a enzima creatina quinase (CK) sanguínea. Além disso, o tempo prolongado na área de espera aumentou o pH final e o valor de a* (teor de vermelho) e diminuiu a luminosidade dos filés. A interação significante dos fatores tempo de espera de quatro e seis horas e a posição superior das caixas de transporte propiciaram o desenvolvimento de carnes duras, firmes e escuras (DFD) em frangos de corte.(AU)

Influence of three types of scaffolds on biological behavior of stem cells from human exfoliated deciduous teeth / 实用口腔医学杂志

Objective:To investigate the adhesion,proliferation and differentiation of the stem cells from human exfoliated deciduous teeth(SHEDs)on 3 different types of hydroxyapatite(HA)composite scaffold materials.Methods:Pulp cells from human exfoliated de-ciduous teeth were harvested from impacted deciduous teeth by enzyme digestion,expanded and cultured.Cells were verified by immuno-histochemical methods and in vitro differentiation test.Then the cells were cultured on HA/beta tricalcium phosphate (HA/TCP),HA/collagen (HA/COL)and HA/poly-ethylene propylene lactide (HA/PLGA)scaffold respectively.Adhesion rate was examined at hour 4,6,8 and 10 of culture,proliferation was observed by MTT assay on day 1,4,7 and 10 of culture,respectively.The osteogenic dif-ferentiation was studied by alkaline phosphatase(ALP)test,Von Kossa staining and calcium content measur.Results:The attachment of SHEDs was significantly lower on the HA/COL than on the other 2 scaffolds(P <0.05).The ALP activity,mineralization and calci-um content were the highest on HA/PLGA,and the last on HA/COL(P <0.05).Conclusion:HA/PLGA scaffold is more effective in the promotion of the proliferation,attachment and differentiation of SHEDs than HA/TCP and HA/COL scaffolds.

Investigation of modified platelet-rich plasma (mPRP) in promoting the proliferation and differentiation of dental pulp stem cells from deciduous teeth

Stem cells from human exfoliated deciduous teeth (SHEDs) have great potential to treat various dental-related diseases in regenerative medicine. They are usually maintained with 10% fetal bovine serum (FBS) in vitro. Modified platelet-rich plasma (mPRP) would be a safe alternative to 10% FBS during SHEDs culture. Therefore, our study aimed to compare the proliferation and differentiation of SHEDs cultured in mPRP and FBS medium to explore an optimal concentration of mPRP for SHEDs maintenance. Platelets were harvested by automatic blood cell analyzer and activated by repeated liquid nitrogen freezing and thawing. The platelet-related cytokines were examined and analyzed by ELISA. SHEDs were extracted and cultured with different concentrations of mPRP or 10% FBS medium. Alkaline phosphatase (ALP) activity was measured. Mineralization factors, RUNX2 and OCN, were measured by real-time PCR. SHEDs were characterized with mesenchymal stem cells (MSCs) markers including vimentin, CD44, and CD105. mPRP at different concentrations (2, 5, 10, and 20%) enhanced the growth of SHEDs. Moreover, mPRP significantly stimulated ALP activity and promoted expression of RUNX2 and OCN compared with 10% FBS. mPRP could efficiently facilitate proliferation and differentiation of SHEDs, and 2% mPRP would be an optimal substitute for 10% FBS during SHEDs expansion and differentiation in clinical scale manufacturing.

Efecto de Beauveria bassiana y Beauveria brongniartii en el control de moscas (Musca domestica) en condiciones de laboratorio y en galpones avícolas / Effect of Beauveria bassiana and Beauveria brongniartii for the house fly (Musca domestica) control on laboratory and poultry sheds conditions

Se realizó un experimento en el estado Trujillo, Venezuela con el objetivo de evaluar en condiciones de laboratorio y en galpones avícolas la patogenicidad de Beauveria brongniartii (cepaLF-05) y Beauveria bassiana (LF-08) sobre Musca domestica. Se determinó que a la dosis de 1,2 x107 conidiosporas/mL para B. brongniartii y B. bassiana los tiempos letales (TL50 y TL95) fueron5 y 9 días y 5 y 6 días, respectivamente. En condiciones de campo las esporas de B. brongniartiiy B. bassiana fueron nebulizadas en el interior de galpones de cría de pollos con una densidad de 7 pollos/m2, a dosis de 9 x 107 conidias/mL en 15L de agua por cada 1200 m2; el porcentaje de reducción poblacional después de nebulizar una vez por semana, durante tres semanas, fue de 13,99 y 100% para B. brongniartii y -35, 91 y 100% para B. bassiana

An experiment was carried out in Trujillostate, Venezuela, in laboratory and poultry shedsconditions in order to study the Beauveria brongniartii(strain: LF-05) and Beauveria bassiana (strain: LF-08) pathogenicity in Musca domestica. With 1.2 x 107conidiospores/mL the lethal times (TL50 and TL95)were 5 and 9, and 5 and 6 days, respectively. In fieldconditions spores of B. brongniartii and B. bassianawere applied inside the poultry sheds (7 chickens/m2)with a dose of 9 x 107 conidia/mL in 15L of water foreach 1200 m2, the percentage of population reductionwere induced after vaporized once a week for threeweeks in 13.99 and 100% for B. brongniartii; and -35,91 and 100% for B. bassiana