Remoción anaerobia del colorante azul directo brl en Reactor Anaerobio de Flujo Ascendente UASB (Upflow Anaerobic Sludge Blanket) con carbón activado / Anaerobic removal of the brl direct blue dye in Upflow Anaerobic Sludge Blanket (UASB) with activated carbon

En esta investigación se utilizó el colorante azul directo brl para remoción anaerobia con un consorcio bacteriano aislado de efluentes industriales del Parque Industrial río Seco (PIRS), Arequipa, Perú; en un reactor anaerobio de flujo ascendente UASB con carbón activado. El reactor tuvo una capacidad de 14.4 L con lodos y carbón activado del 40% de volumen, con una carga orgánica de 6 Kg DQO/m³•día y un tiempo de retención hidráulica de 1 día con un flujo ascendente. El objetivo fue medir la eficiencia de la remoción anaerobia del colorante en un tiempo de 28 días. Los resultados demostraron un incremento del 41% de los Sólidos Suspendidos Volátiles (SSV) de 12894 mg•L-1 a 21546 mg•L-1 bajo las condiciones del experimento, con una remoción del 57% de la Demanda Química de Oxigeno (DQO) de 484 mg•L-1 a 122 mg•L-1 y una remoción del 87% del colorante azul directo brl de 69.61 mg•L-1 a 9 mg•L-1. Los resultados con el carbón activado granular solamente, demostraron una remoción del 61% del colorante azul directo brl de 70.67 mg•L-1 a 27.83 mg•L-1 a los 28 días.

In this research the brl direct blue dye was used for anaerobic removal with a bacterial consortium of industrial effluents from Industrial Park Río Seco (IPRS), Arequipa, Peru; in an anaerobic reactor of UASB Upflow with activated carbon. The reactor had a capacity of 14.4 L with sludge and activated carbon of 40% of volume, with an organic load of 6 Kg COD/m³•dia and a hydraulic retention time of 1 day with an upward flow. The objective was to measure the efficiency of the anaerobic removal of coloring in a time of 28 days. The results showed an increase of 41% of the solids suspended volatile (SSV) 12894 mg•L-1 up to 21546 mg•L-1 under the conditions of the experiment, with a removal of 57% of the chemical demand of oxygen (COD) from 484 mg•L-1 to 122 mg•L-1 and a removal of 87% of the dye Blue direct the 69.61 brl mg•L-1 to 9 mg•L-1. Results with activated charcoal granular only, they showed a removal of 61% of the dye Blue direct 70.67 brl mg•L-1 to 27.83 mg•L-1 at 28 days.

Clinical Study of Endovenous Laser Treatment in Short Saphenous Vein Varicosity

PURPOSE: With regard to the selection of varicose treatment, conventional therapy such as stripping and ligation has became unpopular but the selection of endovenous radiofrequency coagulations (RF) or endovenous laser treatment (EVLT) of the greater saphenous varicose vein have increased as they are more convenient for patient. Mipiphlebectomy (MP) and sclerotherapy (SC) have also been welcome due to the advantages of less scaring and a rapid return to work. Therefore, varicose vein of the greater saphenous vein (GSV) and its tributaries are well controlled by new modalities, such as RF, EVLT, MP and SC, but those of the short saphenous vein (SSV) are difficult to control because of the many tributaries and perforating veins. A new modality with a simultaneous EVLT +/-MP combination for the treatment of SSV varicosities has been used. METHOD: 27 cases of SSV varicosities in 24 patients, between Feb 2002 and May 2004, were included in this study. The series of patients were followed up for recurrence of varisose vein, SSV recanalization and complications. RESULT: The patients were grossly examined postoperatively, and doppler were checked, and the varicose recurrence rates at 1, 3 and 6 months were found to be 0, 7.4 (2/27) 25.9% (7/27), espectively, the Doppler SSV recanalization rates at 1, 3 and 6 months were 0, 0 and 11.1% (3/27) and tributaries recurrence rates were 0, 7.4 (2/27) and 14.8% (4/27) with recurrence controlled by MP or SC, or observed for progression only. Most patients (93.6%) were satisfied with our treatment modality, and complications, such as pain, hematoma and swelling were controlled within 1 month, with 3 cases of tingling sensation of calf that subsided spontaneously. CONCLUSION: The control of SSV varicosities wes a little hard due to the many recurrences after surgery or EVLT alone. In our study, the cases of SSV varicosity alone were collected, and combination therapy, such as EVLT with miniphlebectomy, wes effective, as indicated by the relatively few recurrences, and even in these recurrence cases, additional postoperative MP or SC was enough for patient's satisfaction.

SERUM-FREE CULTURE OF SSV-NIH3T3 CELLS / 解剖学报

The establishment of a serum-free culture medium (SFM) for SSV-NIH3T3 cells is reported in this paper. In this medium DMEM/Ham's F12 is the fundamental element in basal medium, insulin and undefatty bovine serum albumin are key elements among the supplements. In this SFM SSV-NIH3T3 cells grow well, they keep the ability of secreting platelet-derived growth factor-like material into culture medium and causing tumor growth in nude mice.