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1.
Rev. bras. parasitol. vet ; 30(3): e003621, 2021. graf
Artículo en Inglés | LILACS, VETINDEX | ID: biblio-1288706

RESUMEN

Abstract The macroscopic, histological, and molecular aspects of Sarcocystis spp. were examined in the tissues of two cattle and four sheep, 16 and eight fragments analyzed respectively, condemned in the slaughterhouse. All 24 samples were collected and analyzed for detecting macrocysts and macroscopic lesions. Subsequently, subdivided for direct examination, polymerase chain reaction and histopathological examination. All sheep tissues samples had grossly white round to oval tissue cysts, ranging from 0.3 to 1 cm in diameter. In contrast, cattle tissues did not present grossly visible cysts but had randomly distributed white-yellow foci with irregular contours. All samples from cattle and sheep had microscopic cysts. In the histological examination of sheep tissues, circular to elongated, encapsulated, basophilic structures ranging from 30 to 3,000 µm in length and 20 to 1,000 µm in width were observed within the skeletal muscle fibers. In cattle tissues, all cardiac muscle four fragments analyzed contained circular to elongated basophilic structures inside cardiomyocytes and in some Purkinje fibers. PCR were performed using the primers: 2L and 3H. In conclusion, all 24 tissues were infected with Sarcocystis spp., and S. gigantea (in sheep) and S. cruzi (in cattle). were the identified species by sequencing.


Resumo Os aspectos macroscópicos, histológicos e moleculares de Sarcocystis spp. foram examinados nos tecidos de dois bovinos e quatro ovinos, 16 e oito fragmentos analisados, respectivamente, condenados no matadouro. Todas as 24 amostras foram coletadas e analisadas para detecção de macrocistos e lesões macroscópicas. Posteriormente, subdivididas para exame direto, reação em cadeia da polimerase e exame histopatológico. Todas as amostras de tecidos de ovelha apresentavam cistos grosseiramente visíveis, caracterizados como brancos, de redondos a ovais e estruturas variando de 0,3 a 1 cm de diâmetro. Em contraste, os tecidos de bovinos não apresentavam cistos grosseiramente visíveis, mas tinham focos branco-amarelos com contornos irregulares, distribuídos aleatoriamente. Todas as amostras de bovinos e ovinos apresentavam cistos microscópicos. No exame histológico de tecidos ovinos foram observadas estruturas basofílicas circulares a alongadas, encapsuladas, variando de 30 a 3.000 µm de comprimento e 20 a 1.000 µm de largura dentro das fibras do músculo esquelético. Nos tecidos de bovinos, todos os quatro fragmentos de músculo cardíaco analisados continham estruturas basofílicas circulares a alongadas, dentro dos cardiomiócitos e em algumas fibras de Purkinje. PCRs foram realizadas utilizando-se os "primers" 2L e 3H. Em conclusão, todos os 24 tecidos estavam infectados com Sarcocystis spp., sendo S. gigantea (em ovinos) e S. cruzi (em bovinos) as espécies identificadas por sequenciamento.


Asunto(s)
Animales , Enfermedades de las Ovejas/diagnóstico , Enfermedades de los Bovinos/diagnóstico , Sarcocystis/genética , Sarcocistosis/diagnóstico , Sarcocistosis/veterinaria , Bovinos , Ovinos , Mataderos
2.
The Korean Journal of Parasitology ; : 121-127, 2018.
Artículo en Inglés | WPRIM | ID: wpr-742241

RESUMEN

To survey the prevalence of Sarcocystis infections, 210 heart samples were collected from Korean native cattle (Bos taurus coreanae) at an abattoir in Daejeon Metropolitan City, Republic of Korea. Sarcocysts were detected form 31 specimens (14.8%) and identified as Sarcocystis cruzi via transmission electron microscopy. The wall of S. cruzi has flattened protrusions that did not contain fibrils or microfilaments. The protrusions arose irregularly from the base, contained a fine granular substance, lacked internal microfilaments, and measured approximately 0.21–1.25 μm in length and 0.05–0.07 μm in width. Sequence analysis revealed 99.5% homology to S. cruzi. This is the first report on the prevalence of S. cruzi in native cattle from the Republic of Korea.


Asunto(s)
Animales , Bovinos , Mataderos , Citoesqueleto de Actina , Corazón , Corea (Geográfico) , Microscopía Electrónica de Transmisión , Prevalencia , República de Corea , Sarcocystis , Análisis de Secuencia
3.
The Korean Journal of Parasitology ; : 339-344, 2016.
Artículo en Inglés | WPRIM | ID: wpr-166323

RESUMEN

The genus Sarcocystis is not usually considered as an important enteric pathogen in immune compromised patients. It might be expected that species for which humans are the final host (Sarcocystis hominis and Sarcocystis suihominis as well as possibly others) would be encountered increasingly often in immunodeficient persons. This study aimed to address how to detect and differentiate Sarcocystis oocysts and/or sporocysts from enteric protozoans in the diarrheal samples of immunodeficient patients in Shiraz, Iran. Diarrheal samples of 741 immunodeficient patients with recurrent persistent or chronic diarrhea were examined by microscopy and molecular biological analysis. Oocysts-positive samples were 68 Cryptosporidium spp., 9 Cystoisospora belli (syn. Isospora belli), 2 Cyclospora cayetanensis, and 15 microsporidia (Enterocytozoon bieneusi). Sarcocystis-like sporocysts found from a woman were identified as Sarcocystis cruzi through 18S rDNA amplification and phylogenetic analysis. To the best of our knowledge, this is the first report of S. cruzi from a human.


Asunto(s)
Femenino , Humanos , Cryptosporidium , Cyclospora , Diarrea , ADN Ribosómico , Irán , Isospora , Microscopía , Microsporidios , Oocistos , Reacción en Cadena de la Polimerasa , Prevalencia , Sarcocystis
4.
Pesqui. vet. bras ; 28(11): 561-564, nov. 2008. ilus, tab
Artículo en Portugués | LILACS | ID: lil-506666

RESUMEN

Esporocistos de Sarcocystis foram identificados nas amostras fecais de um cachorro-do-mato. Eles foram dados por via oral para um bezerro em aleitamento, sendo observados cistos com morfologia compatível com os de Sarcocystis cruzi na musculatura cardíaca e esquelética, três meses após a infecção. Musculatura cardíaca deste bezerro foi dada para um segundo cão doméstico livre de coccídios, que eliminou esporocistos compatíveis com os de Sarcocystis em suas fezes, tendo com períodos pré-patente e patente 11 e 12 dias após a infecção respectivamente. Para comparar a morfologia dos esporocistos e cistos, um segundo cão, também livre de coccídios, foi alimentado com musculatura cardíaca de um bovino infectando naturalmente e positivo para cistos de S. cruzi. Esporocistos compatíveis com os eliminados pelo primeiro cão foram encontrados nas fezes. Apesar dos esporocistos eliminados pelo cachorro-do-mato serem significativamente diferentes dos eliminados pelos cães infectados experimentalmente, pode se considerar com base na morfologia dos esporocistos, cistos e na transmissão biológica que a espécie encontrada nas fezes do cachorro-do-mato é Sarcocystis cruzi.


Sporocysts of Sarcocystis were identified in feces samples of a crab-eating fox, and were orally given to a suckling calf; after 3 months of infection, sarcocysts morphologically similar to Sarcocystis cruzi were observed in cardiac and skeletal striated muscles. The cardiac muscles of this calf were orally given to a puppy free of coccidia, that shed sporocysts in its feces.with a prepatent and patent period of 11 and 12 days after infection, respectively. To compare the morphology of the sporocysts and cysts, a second puppy was fed on bovine cardiac muscles infected naturally, and sporocysts identical to those shed by the first dog were recovered from its feces. In spite of the significant difference between sporocysts found in the mucosa of the crab-eating fox and those shed by the first and second puppies, the species observed in this study was considered to be Sarcocystis cruzi, based on size of the sporocyts, morphology of the cyst wall, and the pray-predator cycle.


Asunto(s)
Animales , Perros , Bovinos , Enfermedades de los Perros/parasitología , Enfermedades de los Bovinos/parasitología , Sarcocistosis/parasitología , Sarcocistosis/veterinaria , Sarcocystis/aislamiento & purificación , Heces/parasitología , Sarcocistosis/parasitología
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