Molecular identification of the first case of aural myiasis caused by Sarcophaga peregrina in an infant from Hainan, China

Objective: To report a case of aural myiasis in an infant and molecular identification of larva species. Methods: A larva was extricated from left external auditory canal of an infant in the Neonatal Department of the People's Hospital of Baisha County, Hainan Province on 25 June 2019. The larva was placed in 70% alcohol and sent to the Department of Pathogenic Biology, Hainan Medical University. The morphological characters of larva was observed under stereoscopic microscope. The genomic DNA of the larva was extracted and amplified by PCR targeting mtDNA cytochrome c oxidase subunit 1 gene of Diptera, then the PCR product was sequenced and analyzed. Results: A 5-day-old male infant with neonatal hyperbilirubinemia became suddenly irritable. Physical examination found bleeding of the left external auditory canal. A larva about 1.0 cm×0.2 cm was found in the left external auditory canal when the left ear was cleaned with saline. The infant was transferred to Hainan Women and Children's Medical Center for further examination. Otoscopic examination found that the right external auditory canal was normal, with a small amount of yellow secretion and intact tympanic membrane. However, the left ear canal was slightly swollen, with a small amount of yellow secretion and no obvious perforation of the tympanic membrane. The child was discharged after four days of treatment with appropriate medication. Morphological characteristics observed by stereoscopic microscope implied that larva was the maggot of Sarcophaga sp. PCR amplification and sequencing analyses confirmed that the larva was Sarcophaga (S.) peregrina. The sequence exhibited 100% homology with the S. peregrina c oxidase subunit 1 gene (GenBank No. AF259509.1). Conclusions: This is the first report of an infant with aural myiasis in Hainan Province. The molecular characteristics suggest that the aural myiasis was caused by S. peregrina. One of the factors causing infant arual myasis might be residual amniotic fluid in the external auditory canal of the newborn.

A Case of Cutaneous Wound Myiasis Associated with Basal Cell Carcinoma by Sarcophaga africa / 대한피부과학회지

No abstract available.

Comparative morphology and identification key for females of nine Sarcophagidae species (Diptera) with forensic importance in Southern Brazil

ABSTRACTThe identification of female flesh flies was always considered a difficult task since morphological descriptions and keys for females are rare. Even in a forensic entomology framework, where females play a major role, female flesh flies are usually not identified. In order to fill this gap in Southern Brazil fauna we provide detailed descriptions and key for the female of nine species included in four genera: Microcerella halli (Engel), Oxysarcodexia paulistanensis (Mattos), Oxysarcodexia riograndensis (Lopes), Peckia (Euboettcheria) australis (Townsend), Peckia(Euboettcheria) florencioi (Prado and Fonseca), Peckia (Pattonella) intermutans (Walker), Peckia(Pattonella) resona (Lopes), Peckia (Sarcodexia) lambens (Wiedemann), and Sarcophaga(Bercaea) africa (Wiedemann). These species are distinguished mainly by genital characters as tergite 6 divided or undivided, presence of tergite 8, spermatheca morphology and vaginal plate shape.

Ultra Structure of the Mid-Gut of the Third Instar Larvae of Sarcophaga Aegyptiaca (Diptera: Sarcophagidae).

The mid-gut of third instar larvae of Sarcophag aegyptiaca was divided into anterior, middle and posterior mid-gut regions. A well-developed peritrophic membrane appeared in the apical part of the anterior and middle portions of mid-gut while it is absent from the posterior portion. The microvilli differs in 3 portions, appeared as apical membrane in the anterior portion, sparse in middle portion to long slender compact microvilli in posterior portion. Numerous organelles were observed throughout the cytoplasm of the 3 portions; lipid spheres, rough endoplasmic reticulum, seceretory vesicles and mitochondria. A large apically nucleus appeared in the 3 portion of mid-gut. A basal labyrinth was observed in both anterior and posterior regions of mid-gut while it was absent in middle portion of mid-gut. Thin basement membrane was observed in the 3 portions of mid-gut of third instar larvae of Sarcophag aegyptiaca.

Traumatic Myiasis Caused by an Association of Sarcophaga tibialis (Diptera: Sarcophagidae) and Lucilia sericata (Diptera: Calliphoridae) in a Domestic Cat in Italy

We describe here a rare case of traumatic myiasis occurred in August 2014, caused by an association of 2 Diptera species, Sarcophaga tibialis Macquart (Diptera: Sarcophagidae) and Lucilia sericata (Meigen) (Diptera: Calliphoridae), in a domestic cat in northern Italy. Species identification was based on adult male morphology. The present case is the first report of S. tibialis as an agent of myiasis in Italy, and also the first ever report of myiasis caused by an association of S. tibialis and L. sericata. The cat developed an extensive traumatic myiasis in a large wound on the rump, which was treated pharmacologically and surgically. The biology, ecology, and distribution of S. tibialis and L. sericata are also discussed. A literature review is provided on cases of myiasis caused by S. tibialis, and cases of myiasis by L. sericata involving cats worldwide and humans and animals in Italy.

Sarcophaga (Liosarcophaga) dux (Diptera: Sarcophagidae): A flesh fly species of medical importance

BACKGROUND: Although tropical climate of Thailand is suitably endowed with biodiversity of insects, flies of medical importance is not well investigated. Using information from literature search, fly survey approach and specialist's experience, we review database of Sarcophaga (Liosarcophaga) dux Thomson (Diptera: Sarcophagidae), one of the priorities flesh fly species of medical importance in Thailand. RESULTS: This review deals with morphology, bionomics and medical involvement. Important morphological characteristics of egg, larva, puparia and adult were highlighted with illustration and/or micrographs. Search pertaining to molecular analysis used for fly identification and developmental rate of larvae were included. Medical involvement of larvae was not only myiasis-producing agent in humans and animals, but associated with human death investigations. CONCLUSIONS: This information will enable us to accurate identify this species and to emphasis the increase medically important scene in Thailand.

Intestinal myiasis.

Purpose: Intestinal myiasis is a condition when the fly larvae inhabit the gastrointestinal tract and are passed out in faeces. This type of infestation results when eggs or larvae of the fly, deposited on food are inadvertently taken by man. They survive the unfavourable conditions within the gastrointestinal tract and produce disturbances, which may vary from mild to severe. The condition is not uncommon and is often misdiagnosed as pinworm infestation. Correct diagnosis by the clinical microbiologist is important to avoid unnecessary treatment. Materials and Methods: We had 7 cases of intestinal myiasis. In 2 cases the larvae were reared to adult fly in modified meat and sand medium (developed by Udgaonkar). This medium is simple and can be easily prepared in the laboratory. Results: Of the 7 larvae, 5 were Sarcophaga haemorrhoidalis, 1 Megaselia species and 1 was identified as Muscina stabulans. Conclusions: S. haemorrhoidalis was the commonest maggot involved. A high index of suspicion is required for clinical diagnosis when the patient complains of passing wriggling worms in faeces for a long period without any response to antihelminthics. The reason for long duration of illness and recurrence of infestation is baffling. The nearest to cure was colonic wash. We feel prevention is of utmost importance, which is to avoid eating food articles with easy access to flies.

Gastrointestinal Myiasis by Larvae of Sarcophaga sp. and Oestrus sp. in Egypt: Report of Cases, and Endoscopical and Morphological Studies

Four cases of gastric or intestinal myiasis are reported. The cases contain 2 males (1 child 10 years old, and 1 adult 40 years old) and 2 females (1 girl 18 years old, and 1 adult 50 years old) from Minia Governorate, Southern Egypt. Three of them, including cases no. 1, 3, and 4, were gastric myiasis, and complained of offensive hematemesis of bright red blood. Minute moving worms, larvae of the fly, were found in the vomitus. On the other hand, case no. 2 had intestinal myiasis, and complained of abdominal distention, nausea, vomiting, and diarrhea. The stool of case 2 was mixed with blood, and minute moving worms were observed in the stool. Endoscopy was performed to explore any pathological changes in the stomach of the patients. The larvae were collected and studied macroscopically, microscopically, and us-ing a scanning electron microscope (SEM) to identify their species. Three different types of larvae were identified. The larvae isolated from case 1 were diagnosed as the second stage larvae of Sarcophaga species, and the larvae isolated from case 2 were the third stage larvae of Sarcophaga species. On the other hand, the larvae isolated from cases 3 and 4 were diagnosed as the third stage larvae of Oestrus species.

Accidental intestinal myiasis caused by genus Sarcophaga.

Myiasis of different organs has been reported off and on from various regions in the world. We report a human case of intestinal myiasis caused by larvae of Sarcophaga. A 25 - year - old male presented with symptom of passage of live worms in stool. Following diagnosis and treatment the patient improved completely with cessation of maggots in stool.

Nosocomial Oral Myiasis by Sarcophaga sp. in Turkey

We present a case of oral myiasis in a 15-year-old boy with tuberculosis meningitis. The diagnosis was based on the visual presence of wriggling larvae about 1 cm in size and on the microscopic features of the maggots, especially those relating to stigmatic structures. The larvae were identified as third stage larvae of Sarcophaga sp.

Miasis nosocomial por sarcophaga spp (Diptera: sarcophagidae) en un paciente geriátrico de Costa Rica / Nosocomial myiasis by sarcophaga spp (Diptera: Sarcophagidae) in a geriatrical patient of Costa Rica

Se informa de un caso de miasis nosocomial en un paciente de 86 años internado en el antiguo Hospital "San Rafael", Alajuela, Costa Rica. El paciente presentaba múltiples complicaciones. Fue sometido a respiración asistida y a la colocación de una sonda nasogástrica. Transcurridos cinco días de su internamiento se evidenció la presencia de larvas de mosca en su cavidad oral, que morfológicamente corresponden a Sarcophaga spp. (Diptera: Sarcophagidae). El presente constituye el primer caso de miasis nosocomial informado en la literatura médica de Costa Rica.

We informed about a nosocomial myiasis case in an 86 years old patient that was admitted in the old Hospital "San Rafael", Alajuela, Costa Rica, with many health problems. This patient received assisted respiration and implantation of a nasogastric probe. After five days of admission, the presence of muscoid larvae in oral cavity was documented. The morphology of collected maggots is compatible with Sarcophaga sp. (Diptera: Sarcophagidae). This is the first case of nosocomial myiasis informed in the medical literature of Costa Rica.

Photoperiodic sensitivity and diapause induction during ovarian, embryonic and larval development of the flesh fly, Sarcophaga argyrostoma.

Sensitivity to the daily photoperiod, particularly with respect to pupal diapause induction, was studied during ovarian, embryonic, and larval development of the flesh fly Sarcophaga argyrostoma. Large flies were shown to have a greater number of primary follicles in their ovaries and to be capable of limited ovarian maturation in the absence of exogenous protein (autogeny). Such ovarian development occurred independently of photoperiod. However, long days experienced during embryogenesis caused more rapid development, and earlier larviposition, than short days. Short days during embryonic and subsequent larval development also induced pupal diapause, whereas long days led to continuous or non-diapause development of the pupae. Pupal diapause could not be induced by photoperiods during the vitellogenic phase of ovarian development. In Sarcophaga argyrostoma, a maternal effect preventing pupal diapause among the progeny of files with a diapause history was not observed.