Infection of high-risk human papillomavirus in patients with abnormal cervical cytology in Liaocheng city, Shandong province / 肿瘤研究与临床

Objectives To investigate the epidemiology of high-risk human papillomavirus (HPV) infection and the common genotypes in Liaocheng city, Shandong province, China, and to evaluate the application value of high risk HPV detection in cervical cytology with different pathological conditions.Methods A total of 19 707 permanent female residents in Liaocheng were recruited who were married or had sexual life, aged from 18 to 70 years old.They were screened for cervical cancer by thinprep liquid-based cytology test (TCT) from January 2013 to January 2014.The screen positive rate was 4.24 ％ (837/19 707), and 785 volunteers aged from 21 to 65 years old were recalled.The xMAP bead-based hybridization and flowcytometry analysis were used for genotyping.The data were analyzed by comparison and description.Results According to TCT, among 785 cases, there were 478 cases of atypical squamous epithelium of unknown significance (ASCUS) and atypical glandular epithelium of unknown significance (AGCUS), 175 cases of low-grade squamous intraepithelial lesion (LISL), 127 cases of high-grade squamous intraepithelial lesion (HSIL), 5 cases of squamous cell carcinoma (SCC) and adenocarcinoma (ACC).The positive rate of high-risk HPV was 62.8 ％ (493/785).The risk age of infection was 26-30 years old (87.7 ％, 71/81) and 51-55 years old (79.7 ％, 51/64), while a low risk one was found in patients older than 55 years old (28.6 ％, 14/54).The top five high-risk subtypes of HPV were HPV16 (21.5 ％, 169/785), HPV52 (12.2 ％, 96/785), HPV58 (9.8 ％, 77/785), HPV33 (9.7 ％, 76/785), HPV18 (7.5 ％, 59/785).Single infection accounted for 45.0 ％ (353/785), while multi-infection for 17.8 ％ (140/785).98 cases were infected by two subtypes, 37 cases by three subtypes, 2 cases by four subtypes, 2 cases by five subtypes and 1 case by six subtypes.Conclusions Compared with pure cervical TCT screening, high-risk HPV infection detection is an effective method for cervical cancer screening, which can improve the specificity of cervical cancer screening and reduce the omission diagnostic rate.In Liaocheng, HPV subtypes 16, 52, 58, 33, 18 and multi-infection are more prevalent.Women belonging to 26-30 or 51-55 years old are identified as high-risk population.Screening is important for this group to discover early cervical lesions.

Evaluación médica previa a la práctica deportiva para deportistas aficionados y de nivel competitivo / Pre - participation screening in sports for leisure and competitive athletes

La actividad física y el deporte reducen la mortalidad general y cardiovascular, pero durante la práctica de ejercicio aumenta el riesgo de muerte súbita que puede ser la primera manifestación de una enfermedad cardiovascular no diagnosticada en deportistas previamente asintomáticos. Las causas de muerte súbita difieren según la edad, en los menores de 35 años se debe a patologías congénitas o hereditarias y en los mayores de esta edad a enfermedad coronaria. En el presente artículo se analizan las causas más frecuentes de muerte súbita, así como los protocolos de evaluación médica previa a la práctica de actividad física o deporte propuestos en la literatura para su prevención en deportistas de diferentes edades y nivel de actividad, desde el competitivo de alto rendimiento hasta el recreativo por salud, y utilización del tiempo libre.

The physical activity and sports decrease cardiovascular and all cause mortality, but during exercise increase risk of sudden death is often the first manifestation of underlying cardiovascular disease in previously asymptomatic athletes. The cause of sudden death is different depending on age, in young people (< 35 years) are genetic or congenital cardiovascular abnormalities and in adult people (> 35 years) is coronary arteries disease. This article is a review of sudden death more habitual causes and the protocol of pre-participation in physical activity and sports medical evaluation that exists in the medical literature in sudden death prevention for athletes of all ages and that participate in leisure and competitive sports.

EXPERIMENTAL STUDY ON MTT ASSAY USED I OR AlJNTICANCER DRUG SCREENNING / 重庆医科大学学报

In this paper the procedure for the study of anticancer drug screenning on colon cancer cell line SW-480 using MTT (tetrazolium dye) assay has been observed in vitro. Our study showed that(l) The optimal target cell concentration was 1-5?10~4 cell/well. (2) The optimal MTT concentration is 5 mgml~(-1) when a range of 0-10 mgml~(-1) was tested. In view of the relatively small increase in sensitivity obtained after 6h, we chose 4h incubation as optiomal incubation time. (3) DMSO and acid isopropyl alcohol were both effective to dissolve the formazan produced but greater solubility and absorbance reading were seen using DMSO. (4) The addition of new born calf serum to the reaction mixture results in a gradual lowering of optical density than that produced by serum-free medium before the addition of DMSO.The assay is simple and rapid with a CV within groups about 10%. when using DMSO as a solvent in this assay, it is necessary to read the plates within 30 mim after adding DMSO. Meanwhile, the chemosensitivity of SW-480 cell to 72h exposrre to four kinds of standard anticancer drugs was different.