Cytotoxic and antimicrobial activities of ethyl acetate extract of mangrove plant Scyphiphora hydrophyllacea C. F. Gaertn— Associated fungi

Association between mangrove plants with fungi has been explored for many biological activities. The purpose of thisstudy was to determine the antimicrobial and cytotoxic activities of endophytic fungi isolated from mangrove plantsScyphiphora hydrophyllacea C. F. Gaertn. The isolation of endophytic fungus from the mangrove has been carriedout by the direct planting method on Sabouraud Dextrose Agar medium. Eleven fungal isolates were recovered andpurified. The isolates were cultivated on the rice medium for 1 month and extracted using ethyl acetate. Eleven ethylacetate extracts were screened for cytotoxic activity by Brine Shrimp Lethality Test method and 3-(4,5-dimethylthiazol2-yl)-2,5-diphenyltetrazolium bromide assay and for antimicrobial activity toward some human pathogenic microbes.The results showed that five (45.5%) isolates of endophytic fungi had antimicrobial activity against the growth ofStaphylococcus aureus, Escherichia coli, and Candida albicans with a diameter from 9.1 to 21 mm. Furthermore, fourextracts (36.4%) were cytotoxic (cell viability < 50%) against T47D cells. Isolate ADSH2 which exhibited the highestcytotoxic activity was identified molecularly as Penicillium chrysogenum. The results of this study indicate that theethyl acetate of endophytic fungi associated with Scyphiphora hydrophyllacea has the potential to be developed asantimicrobial and anticancer compounds.

Chemical constituents from stems and leaves of mangrove plant, Scyphiphora hydrophyllacea / 中草药

Objective: To study the chemical constituents of the stems and leaves of mangrove plant, Scyphiphora hydrophyllacea. Methods: The chemical constituents of S. hydrophyllacea were separated and purified by silica gel, ODS, Sephadex LH-20 gel column chromatographies and preparative HPLC. Their structures were identified by physicochemical properties, spectroscopic analysis, as well as comparisons with the data reported in literatures. Results: A total of 18 compounds were isolated from the 90% ethanol extract of the stems and leaves of S. hydrophyllacea, which were identified as pomonic acid (1), euscaphic acid (2), dammaradienyl acetate (3), erythrodiol (4), lupenyl acetate (5), 30-oxo-lupeol (6), maslinic acid (7), 23-hydroxyursolic acid (8), β-amyrenone (9), acacetin (10), chrysoeriol (11), jaceosidin (12), kumatakenin (13), isosakuranetin (14), taxifolin (15), lyoniresinol (16), medioresinol (17), and balanophonin (18). Compounds 1-9 were triterpenoids, compounds 10-15 were flavonoids and compounds 16-18 were lignans. Conclusion: All compounds are isolated from the genus Scyphiphora for the first time.