Efficacy of modified prostate tip separation technique combined with laparoscopic radical prostatectomy based on propensity score matching / 现代泌尿外科杂志

【Objective】 To explore the efficacy of modified prostate tip separation technique combined with laparoscopic radical prostatectomy based on propensity score matching (PSM) in the treatment of prostate cancer. 【Methods】 A total of 74 prostate cancer patients treated during Jan.2019 and Dec.2022 with modified prostate tip separation technique combined with laparoscopic radical prostatectomy were included in the combined group, and another 63 prostate cancer patients treated during the same period with laparoscopic radical prostatectomy were selected as the control group. Altogether 58 pairs of patients were matched with PSM. The perioperative indicators, incidence of complications, urinary control function and sexual function before and one month after surgery between the two groups after matching were compared. 【Results】 There were no statistically significant differences in general data between the two groups (P>0.05). One month after operation, the scores of the International Urinary Incontinence Questionnaire (ICIQ-SF) and International Erectile Function Questionnaire (IIEF-5) in both groups decreased, while the Expanded Prostate Cancer Index Composite (EPIC-UIN) and International Prostate Symptom Score (IPSS) in both groups increased (P<0.05). The scores of ICIQ-SF [(9.02±1.98) vs. (11.38±2.04)] and IPSS [(19.67±4.19) vs. (21.68±4.23)] were lower in the combined group than in the control group (P<0.05), while the scores of EPIC-UIN [(70.49±6.82) vs. (63.34±6.48)] and IIEF-5 [(18.17±1.73) vs. (16.72±1.58)] were higher in the combined group than in the control group (P<0.05). Compared with the control group, the combined group had shorter catheter retention time [(7.38±1.97) d vs. (5.11±1.82) d] and hospital stay [(13.18±2.23) d vs. (11.74±2.09) d], lower incidence of complications (22.41% vs. 6.90%), and higher positive rate of incision margin (8.62% vs. 20.69%) (P<0.05). 【Conclusion】 PSM can balance the differences between groups. The modified prostate tip separation technique combined with laparoscopic radical prostatectomy can improve the urinary control function, have little impact on sexual function, and cause fewer postoperative complications. However, the risk of positive incision margin is high, and further modification is needed to achieve the best therapeutic effects.

Re-recognition of endoscopic Sublay repair / 中华消化外科杂志

Repair of abdominal hernia is in dynamic evoluation.Laparoscopic intra-peritoneal onlay mesh (IPOM) and open Sublay repair are still the best methods for the repair of abdominal wall hernia. The application of component separation technique has further widened the indications of IPOM and Sublay repair for the treatment of large abdominal wall hernia. Endoscopic Sublay technique theoretically combines the advantages of open Sublay repair and laparoscopic IPOM repair, but it has the disadvantages of iatrogenic destruction of the tendon septum and normal mechanical deconstruction, which requires strict specification of indications and further research.

The value of MRI IDEAL technique in the etiological analysis of vertebral compression fracture / 实用放射学杂志

Objective To discuss the differential value of magnetic resonance asymmetric echo threeGpoint waterGfat separation technique (IDEAL)in benign and malignant vertebral compression fractures.Methods 48 cases of vertebral compression fractures patients (85 vertebrals)were performed conventional MRI and IDEAL scanning at 1.5T superconducting MR scanner (GE Brivo 355 MR system).25 benign and 23 malignant vertebral compression fracture cases were enrolled in this study.25 benign cases(42 vertebrals)included 8 pure traumatic fractures,16 osteoporosis and 1 tuberculosis respectively,while 23 malignant cases (43 vertebrals)included 22 metastases and 1 myeloma respectively.The signal intensity ratios(SIR)of the benign and malignant vertebral in waterGfat imaging and that of inGphase and outGphase (fatty signal intensity/water signal intensity and outGphase signal intensity/inGphase signal intensity respectively),and namely SIR were measured and calculated.SIR in the 4 groups were statistically analyzed.Results WaterGfat SIR of benign vertebral compression fracture was 0.67±0.19,whereas in malignant vertebral compression fractures was 0.14±0.07,and the difference was statistically significant (t＝1 2.6 2 ,P＜0.05 ).SIR of outGphase signal intensity/inGphase signal intensity of benign vertebral compression fracture was 0.52±0.25,whereas in malignant vertebral compression fracture was 0.83±0.07,and the difference was statistically significant (t＝－7.2 9 ,P＜0.05).Conclusion The SIR of malignant group is significantly lower than that of benign group in waterGfat imaging. whereas the SIR of malignant group is higher than that of benign group in inGphase and outGphase imaging.There is no overlap of SIR between benign and malignant in waterGfat imaging and inGphase and outGphase imaging.IDEAL technique has a high application value in the identification of benign and malignant vertebral compression fractures,which is worth popularizing.

Fluorous Solid-Phase Extraction Assisted New Methods for Analysis of Biomolecules with Mass Spectrometry / 分析化学

Fluorous solid-phase extraction ( FSPE ) is a solid-phase extraction technique based on fluorous affinity between perfluorous compounds. It requires derivatization on analytes with fluorous tags and further specific separation accomplished by perfluorinated solid phase. This technique has extended to various research fields with broad application including organic synthesize, catalysis, chemical and biological analysis. Recently, owing to its good compatibility with mass spectrometry, new analytic techniques relying on FSPE coupled to biological mass spectrometry have received wide attention. This review briefly introduced the principle of FSPE and emphasized on its application for the analysis of biomolecules with mass spectrometry, as well as its outlook of future development.

Re-evaluation on purification process of Qingnao Fushen Oral Liquid / 中草药

Objective: To reevaluate the purification process of Qingnao Fushen Oral Liquid (QFOL). Methods: The orthogonal experiment of adsorption process of the activated carbon was optimized with the diversion rates of berberine and total flavonoids and the reduction of solid content as indexes. The aperture, pressure, temperature, and collection were optimized with the diversion rates of berberine and total flavonoids and the reduction of solid content as indexes. At the same time, the method of membrane cleaning was screened through the combination of different cleaners. Results: The adsorption process of activated carbon was as following: the usage of activated carbon was 0.1%, at normal atmospheric temperature, and the adsorption time was 20 min. The optimal process was aperture 200 nm, pressure 0.10 MPa, temperature 40℃, and collection 1.25 times of the original volume. The cleaning method of membrane is to use NaOH to clean for 30 min and HNO3 to clean for 30 min. Conclusion: The purified QFOL can be obtained by this technique, which is helpful for industrialization.

Strategies for functional repair of complex abdominal wall defects / 中华消化外科杂志

It is still a challenge for surgeons to deal with the complex abdominal wall defect.The purpose of surgical treatment is not only to restore the integrity of the abdominal wall, but also to maintain the function of the abdominal wall.Accurate classification and partition of the abdominal wall defects before operation are the base of surgical procedure for complex abdominal wall defects.Reinforcement of the abdominal wall defects with mesh, component separation technique and tissue flap technique are important methods to achieve functional repair of the abdominal wall defects.

Double Tunnel Separation Technique in Laparoscopic Radical Resection for Rectal Carcinoma / 中国微创外科杂志

Objective To discuss the value of double tunnel separation technique in the application of laparoscopic radical resection for rectal carcinoma. Methods A retrospective analysis of clinical data of 67 cases of laparoscopic radical resection for rectal carcinoma from September 2010 to July 2014 was made.There were 37 cases of Miles operation and 30 cases of Dixon operation, with laparoscopic double tunnel separation technique which refered to under the sigmoid colon mesentery space and under the mesorectum space. Results All cases of laparoscopic radical resection were completed.The duration of operation was 100-240 min, with an average of 158 min.The intraoperative blood loss was 50-200 ml, with an average of 110 ml.The hospitalization time was 8-15 d, with an average of 10 d.Complications included postoperative anastomotic leakage in 1 case, small intestinal obstruction induced by intestine pelvic adhesion in 1 case, and lower extremity deep venous thrombosis in 1 case, all of which were cured with conservative treatment. Conclusions Double tunnel separation technique in laparoscopic radical resection is helpful to application of total mesorectal excision, thus effectively protects blood vessels, nerves, and ureters.Meanwhile, the lymph nodes and extranodal tumor deposits can be cleaned to the greatest degree.

Clinical study of open versus endoscopic component separation with biological mesh reinforcement in treatment of patients with abdominal wall defects / 国际外科学杂志

Objective To compare the operation effect of endoscopic versus open component separation on abdominal wall reconstruction.Methods From January 2012 to December 2013,19 patients (ECST 8 and CST 11) with abdominal wall defects were repaired with biomaterials mesh.This study analyzed retrospectively the clinical information between two groups.Results No hernia recurrences were occurred during a mean follow-up of (14.96± 8.89) months.There was no significant difference in EBL,operation time,length of stay.The rate of recurrence wound complications in CST group is higher than the ECST group,but is not significant difference.Conclusions Endoscopic or open component separation with biomaterials mesh could effectively repair the abdominal wall defect and ECST definitely showed the advantage in reducing the incisional complication.But the large sample,long-term clinical follow-up should be confirmed.

Nanometrology and its perspectives in environmental research

OBJECTIVES: Rapid increase in engineered nanoparticles (ENPs) in many goods has raised significant concern about their environmental safety. Proper methodologies are therefore needed to conduct toxicity and exposure assessment of nanoparticles in the environment. This study reviews several analytical techniques for nanoparticles and summarizes their principles, advantages and disadvantages, reviews the state of the art, and offers the perspectives of nanometrology in relation to ENP studies. METHODS: Nanometrology is divided into five techniques with regard to the instrumental principle: microscopy, light scattering, spectroscopy, separation, and single particle inductively coupled plasma-mass spectrometry. RESULTS: Each analytical method has its own drawbacks, such as detection limit, ability to quantify or qualify ENPs, and matrix effects. More than two different analytical methods should be used to better characterize ENPs. CONCLUSIONS: In characterizing ENPs, the researchers should understand the nanometrology and its demerits, as well as its merits, to properly interpret their experimental results. Challenges lie in the nanometrology and pretreatment of ENPs from various matrices; in the extraction without dissolution or aggregation, and concentration of ENPs to satisfy the instrumental detection limit.

Clinical application of component separation technique in hernia surgery / 国际外科学杂志

Component separation technique in recent years both at home and abroad is mainly used in the repair of abdominal wall defects and abdominal wall reconstruction,especially for the great defect of the abdominal wall repair.Combined with laparoscopic minimadly invasive techniques,synthetic patch reflects the technology advantage in the abdominal wall reconstruction.This paper focuses on the surgical principle of component separation technique,indications,advantages,methods of operation,complications and related progress

Components separation technique for large abdominal wall defect / 대한외과학회지

Repairing large incisional hernia with abdominal wall reconstruction is a technically challenging problem for surgeons. We report our experience of large midline incisional hernia which was repaired successfully with components separation technique. A patient with incisional hernia, 35 x 20 cm in size, underwent operation following standard components separation technique. The aponeurosis of the external abdominal oblique muscle was longitudinally transected from the rectus sheath, and the external abdominal oblique muscle was separated from the internal abdominal oblique muscle. With further separation of the posterior rectus sheath from the rectus abdominis muscle, closure of the abdominal wall was attained without tension. The post-operative course was uneventful with minor wound seroma. The patient discharged safely, and no further complication in terms of recurrence and wound problem has occurred. Components separation technique could be a possible and effective treatment option for repair of large abdominal wall defect.