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Introduction:Sacoglottis gabonensis (Baille) Urban (Humiriaceae) is a medicinal plant used in the treatment of Buruli ulcer in Ivory Coast. To ensure its effect over along period of use, the subchronic toxicity of the total aqueous extract of S. gabonensisstem bark (ETASg) in rats was evaluated.Methods:80 rats were homogeneously distributed in 4 lots of 20 rats each, including 10 males and 10 females. ETASg was administered daily orally for 90 days for 2 mL/100 g body weight (b.w.) rats at doses of 3.5; 35 and 350 mg/kg b.w. The control group received distilled water. A venous blood sample is taken every 30 days for 90 days to determine serum biochemical parameters Results:The study showed that ETASg did not influence serum biochemical markers at the therapeutic dose of 3.5 mg/kg b. w. significant increases in ASAT serum activity, ALT, PAL, and LDH in rats tested at 35 and 350 mg/kg b. w. was found on the 60th and 90th days. Administration of ETASg did not affect most of the metabolites and electrolytes studied at doses of 3.5; 35 and 350 mg/kg b. w. After 30 days of discontinuing oral administration of ETASg, the increase observed at the 350 mg/kg b. w. is moderate and reversible.Conclusion:This study revealed that ETASg is nontoxic for biochemical parameters, at doses of 3.5; 35 and 350 mg/kg b. w. during 90 days of administration in rats
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Objective To learn the changes of blood lead levels and serum biochemical parameters of the school agechildren from different areas. Methods All research objects, the school age children, were from three different areasrespectively, including a mountainous area (L area), an island area (H area) where there is not history of Pb pollution,and an industry area (N area) in relation to Pb pollution. The morning urine and peripheral venous blood samples werecollected from the school age children. Pb in blood (PbB), δ-aminoaevulinic acid in urine (ALA), Ca2+, BUN, Cr inserum, and Thyroid Stimulating Hormone (TSH), thyroxin (T4), free thyroxin (FT4) levels were detected. ResultsPbB levels [M was 36.0 ppb] of the school age children from N area were significantly higher than that of L area [22.0 ppb] andH area [23.8 ppb]. On the contrary, serum Ca2+ levels of the school age children from N area were significantly lower than thatof L area and H area. Serum T4 of N area was significantly lower than that of L area and H area. Serum FT4 of H area wassignificantly higher than that of L area and N area. And TSH of N area and H area were both obviously lower than that of L area.But all of these thyroxin indexes were in the range of normal values. Conclusion It should be widely concerned that thesignificant elevation of PbB levels may have a negative impact on school age children in the future.
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Objective To determine the hematological and serum biochemical parameters of 50 healthy African green monkeys,and to analyze the difference of these values between different groups of sex and age(the juvenile group:1- 3 years old,the adult group: 4 - 6 years old). Methods Blood samples were obtained from the monkeys in the awake state,and the hematological and serum biochemical parameters of the samples were measured using an automatic blood cell analyzer and blood biochemical analyzer, respectively. Results Among all of the hematologic values, the number of red blood cells(RBC), the amount of hemoglobin(HGB), hematocrit(HCT), and percentages of the neutrophils(NEUT%),lymphocytes(LYMPH%), monocytes(MONO%)and basophils(BASO%)were significantly different between the juvenile group and the adult group(P < 0.05). In the juvenile group, parameters such as the number of the white blood cells(WBC),RBC,HGB,HCT,NEUT%,LYMPH%,MONO% were significantly different between the female and the male monkeys(P < 0.05). As for the adult group, the HCT value was of significant difference,while the other indexes were not significantly different between the female and male monkeys(P < 0.05). Among the serum biochemical values, the quantities of albumin(ALB), aspartate aminotransferase(AST), alkaline phosphatase(ALP)and creatinine(CREA)were significantly different between the juvenile group and adult group(P <0.05). The amount of CREA of the juvenile females was significantly different from that of the juvenile males(P <0.05),and the values of total protein(TP)and cholesterol(CHOL)of the adult females were significantly different from those of the adult males(P < 0.05), while the other indexes were not significantly different. Conclusions As a reference,the routine biological data of African green monkeys we have established in this study lay a foundation for the evaluation of their biological properties and further application in related studies.
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This study investigated changes in certain blood parameters in calves and pigs after foot-and-mouth disease (FMD) vaccination. In this study, five calves and five pigs were selected from groups of 10 calves and pigs, respectively, and were vaccinated with an FMD vaccine. The remaining animals formed two non-treatment control groups. Blood samples were collected from all animals on the 1st, 3rd, 5th, and 7th days post-vaccination. In the FMD-vaccinated calves and pigs on day 7 post-vaccination, white blood cell counts, blood urea nitrogen levels, and alanine aminotransferase and aspartate aminotransferase activities were higher than those in the respective controls. The present data suggested that the certain hemato-biochemical parameters on cattle and pigs were meaningfully changed between before and after FMD vaccination.
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Animales , Bovinos , Alanina Transaminasa , Aspartato Aminotransferasas , Nitrógeno de la Urea Sanguínea , Fiebre Aftosa , Pruebas Hematológicas , Recuento de Leucocitos , Porcinos , VacunaciónRESUMEN
This paper aims to describe the hematological and biochemical values of wild hybrid marmoset (Callithrix penicillata and C. geoffroyi) found in a forest zone of Southeastern Brazil. The marmosets were anaesthetized using ketamine and xylazine hydrochloride. Blood samples (0.5-1mL) were collected through the venipuncture of the femoral vein. Hematological and biochemical analyses were performed using automated counters and biochemical kits. The comparison for sex (adult males vs. adult females) and age class (juvenile vs. adult) physiological data and weight were analyzed through Student's t-test for independent samples. Significant differences between sex were observed in erythrocytes (P<0.01) and hemoglobin (P<0.05). The present study provides essential baseline information on the normal blood values of wild hybrid marmosets, the data of which are not readily accessible from the existing body of scientific literature on nonhuman primates.
Este artigo objetiva descrever os valores hematológicos e bioquímicos de saguis híbridos selvagens (Callithrix penicillata e C. geoffroyi) encontrados em uma zona florestal do Sudeste do Brasil. Os saguis foram anestesiados usando cetamina e hidrocloridrato de xilazina. As amostras de sangue (0,5-1mL) foram coletadas por punção da veia femoral. As análises hematológicas e bioquímicas foram realizadas por meio de contadores automáticos e kits bioquímicos. A comparação dos dados fisiológicos e pesos para o gênero (machos vs. fêmeas) e a classe de idade (juvenil vs. adulto) foram analisados através do teste t de Student para amostras independentes. Observaram-se diferenças significativas de gênero para eritrócitos (P<0,01) e hemoglobina (P<0,05). O presente estudo fornece informação básica essencial sobre os valores hematológicos normais de saguis híbridos selvagens, dados que não são prontamente disponíveis na literatura científica atual sobre primatas não humanos.
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Objective To establish the normal reference value range of hematological and serum biochemical parameters in Beagle dogs for our own research center and to supply references for establishing the national standard.Methods CV Beagle dogs aged 4～6 months were selected as the investigative objects.Of the Beagle dogs,263 were male with body weight of(8.09?0.78)kg,263 were female with body weight of(6.84?0.87)kg.Blood was collected from cephalic vein at the endgame of the experiment,then both hematological and serum biochemical parameters were measured.The acquired data were analyzed by the "Independent-Samples T Test" process of SPSS V 13.0.Regarded the males and the females as two independent samples,the difference between sexes was also compared.Results The mean normal reference values and the standard deviation of hematological and serum biochemical parameters in both male and female Beagle dogs were obtained by analysis.Conclusions Comparison of sexes showed that there exists obviously difference between male and female Beagle dogs referring to 3 serum biochemical parameters of T-Bil,GLU and ALB.